查询词典 RT.

All the synovia specimens were harvested for the RT-PCR method to detect the mRNA expression of MMP-1、MMP-3、IL-1β and TIMP-1, to see the effect of estrogen on the OA cartilage and the possible pathogenesis of estrogen.

对兔膝关节OA滑膜组织中MMP-1、MMP-3、IL-1β和TIMP-1 mRNA水平进行RT-PCR半定量测定，了解雌、孕激素对OA软骨中上述指标的表达情况，以了解雌激素对OA的确切作用，探讨其可能的作用机制。

RT-PCR show an increase expression of mRNA of MMP-1, MMP-3 and IL-1β in OA synovia tissue in ovariectomized rabbits. Estrogen supplement could decrease the expression, an enhancement effect could observed with progestin.

RT-PCR法半定量检测结果发现，去势后兔膝OA滑膜组织中MMP-1、MMP-3和IL-1β mRNA表达增加，给予雌激素对三者的表达均有抑制作用，加用孕激素可使这一抑制作用增强。

The LAMP method was developed in this experiment and it was more sensitive than RT-PCR method for detecting TAV.

建立了检测番茄不孕病毒的LAMP技术；LAMP比RT-PCR具有灵敏度更高、快速、简便等优点。

The virus had serological relationship with IgG of TAV. A pair of primers were designed and synthesized based on the nucleotide sequence of coat protein gene of TAV from England. The expected size of 886 bp was amplified by RT- PCR.

根据英国报道的番茄不孕病毒CP基因序列，设计合成一对引物，对该病毒RNA进行RT PCR扩增，得到与预期大小相符的特异扩增带。

Using TAV CP cDNA clones as templates, the reaction conditions such as Mg(superscript 2+ dNTPs, betaine and primers concentrations optimized and the sensitivities of LAMP and RT-PCR were compared in detecting TAV.

设计能识别TAV CP序列6个特定区域的4种LAMP特异引物，对MgCl2、dNTPs、甜菜碱等LAMP反应条件进行优化，利用新建的LAMP法检测TAV，比较LAMP和RT-PCR2种不同方法的检测灵敏度。

Methods: total rna was isolated from hepatocellular carcinoma cell line hepg2. subsequently, a 1kb fragment of hccr 2 encoding region was amplified by rt pcr and cloned into promega teasy vector. after confirmed by dna sequencing, the full length encoding region of hccr 2 was amplified by pcr and cloned into pires2 egfp. the recombinant eukaryotic expression vector was confimed by dna sequencing.

从人肝癌细胞株hepg2提取rna，利用rt pcr方法，先克隆出一包括hccr 2编码区的长约1 kb的片段，并将其与t载体连接并转化，测序证实无误后，以hccr 2/t载体为模板，再通过pcr克隆出hccr 2的编码区序列，将其连接到pires2 egfp真核表达载体，并通过测序获得证实。

In vitro transcripts from RNA5 wild type clone were co-inoculated into Tetragonia expansa with the RNAs of Hu3 isolate containing RNA1, 2 and 3. Following RT-PCR and Northern blotting results shows RNA5 could be biologically replicated in the leaves. The virus concentration of the isolate containing RNA5 in T. expanxa was higher than that of the isolate Hu3 evaluated by ELISA detection.

野生型RNA5的体外转录物与含Hu3(RNA1+2+3)分离物的总RNA混合接种番杏叶片的RT-PCR和Northern blot检测结果都表明，野生型RNA5能够在接种寄主体内复制，并且ELISA检测证明含有RNA5的番杏病叶中病毒浓度高于不含RNA5病叶的病毒浓度。

RT-PCR and SABC immunohistochemical technique were applied to detect the expression of Tiam 1 mRNA and protein in MO, ML and MH cells.

应用RT-PCR和SABC免疫组化技术分别检测Tiam 1 mRNA与蛋白在MO、ML、MH细胞中的表达。

This introductory chapter will explain what ticketing is and what RT is.

这个介绍章节将解释票务是什么和RT是什么。

Methods: The specimens of peripheral blood were collected from 15 individuals and each specimen was divided into three parts, one processed in an hour and the other two stored at -80℃ and -20℃ respectively Two different reagents (TRI Reagent and TRI Rcagent-BD) were used to extract RNA directly from whole blood followed by reverse-transcription polymerase chain reaction for amplifying 3-Actins gene. The RT-PCR products were detected by the ultraviolet transilluminator after 1.5% agarose gels electrophoresis and being stained by ethidium bromide.

采集人体外周血标本15例，分成三份，分别作为新鲜血组、-80℃冻存血组和-20℃冻存血组标本，用TRI Reagent和TRI Reagent-BD两种不同的试剂提取RNA，以RT-PCR方法扩增β-Actin基因，经1.5%琼脂糖凝胶电泳，溴化乙锭染色，紫外透射仪下观察分析。

And Pharaoh spoke to Joseph, saying, Your father and your brothers have come to you.

47：5 法老对约瑟说，你父亲和你弟兄们到你这里来了。

Additionally, the approximate flattening of surface strip using lines linking midpoints on perpendicular lines between geodesic curves and the unconditional extreme value method are discussed.

提出了用测地线方程、曲面上两点间短程线来计算膜结构曲面测地线的方法，同时，采用测地线间垂线的中点连线和用无约束极值法进行空间条状曲面近似展开的分析。