查询词典 RT.

Methods RT-PCR, Western blot were used to detect the expression of bax in human gastric cancer cells line SGC-7901, and observe the effect of rhIL-24 on chorioallantoic membrane.

采用RT-PCR法和Western blot法检测rhIL-24对胃癌细胞株SGC-7901中促凋亡因子bax表达的影响，鸡胚绒毛尿囊膜技术观察其对血管形成的影响。

According to amino acid sequence to vitellin which has the structural character of GL/ICG motif near C end, two degenerate primers were designed and Chrysopa septempunctata s RNA was extracted, and a 900bp fragment was amplified by RT-PCR.

根据昆虫卵黄蛋白氨基酸序列在靠近C端存在GL／ICG区域的结构特点，设计了两条简并引物，提取大草蛉的RNA，用Takara的3′—RACE试剂盒进行RT-PCR，扩增出一段900bp的片断。

HSP 70 mRNA expression in the posterior cingulate cortex was detected by using semi-quantitative RT-PCR technique; HSP 70 protein expression in posterior cingulated cortex was determined by immuno-histochemical method.

于用药后24 h，大鼠断头取脑，用半定量RT-PCR技术和免疫组织化学方法检测各组HSP70 mRNA和HSP 70蛋白在大鼠后扣带回皮质区的表达。

Methods: Five 10-23 deoxyribozymes (DZ1-DZ5) with 2 phosphorothioate groups at 5' and 3' end were designed and synthesized. Full sequence ET-1 RNA was transcripted and FAM-labeled 10-23 deoxyribozyme was used to select cleavable 10-23 deoxyribozyme in vitro and to detect intracellular uptake. The content of ET-1 mRNA was measured by semi-quantitative RT-PCR after 10-23 deoxyribozyme was transfected into cultured neonatal rat cardiomyocytes.

体外转录ET-1全长RNA底物，设计并合成5条ET-1 10-23脱氧核酶(DZ1~DZ5)，其5'及3'端各有2个核苷酸硫代修饰，体外切割ET-1RNA底物，筛选有效脱氧核酶；5'标记荧光素FAM的10-23脱氧核酶瞬间转染新生大鼠心肌细胞以观察对10-23脱氧核酶的摄取；采用半定量RT-PCR检测ET-1基因的表达。

Further, with a semi-quantitative RT-PCR technique, the temporal expression of UuMAPKKK-like in U. unicinctus coelomic fluid cells was measured after stimulated by sulfide. The mRNA transcript of UuMAPKKK-like was low in the control and short time stressed (2 h, 6 h) groups, up-regulated gradually after 12h stimulation, and then reached its maximum level at 48h.

进一步采用RT-PCR技术对其在硫化物刺激前后的表达进行了检测，结果显示该基因在对照和应激后2h、6h的个体中表达较弱；刺激12h后表达量增高，并随应激时间增加，呈明显上调趋势。

A cDNA for endo-β-1,4-glucanase was isolated by RT-PCR, and rapid amplification of cDNA ends reaction from taro leaves (Colocasia esculenta var. esculenta).

本研究利用RT-PCR及rapid amplification of cDNA ends的方法，自槟榔心芋叶片选殖内切型纤维素分解酵素cDNA。

Then the combinant plasmid was conducted into ACC-2 cell by electroporation. ACC-2 cells stably expressing CD was obtained by 10-day positive selection with 400 μg/mL G418. Total RNA was extracted and the expression of the CD gene in transfected ACC-2 cells was identified by RT-PCR. RESULTS: PCR yielded a fragment of l280bp and CD was verified by sequence analysis.

测序正确后，将其亚克隆到质粒表达载体pIRES中，构建以内部核糖体进入位点相连的CD基因的质粒表达载体pIRES-CD，采用电穿孔法，以质粒表达载体转染ACC-2细胞，用400μg/mL的G418筛选10d，获得稳定表达CD基因的ACC-2细胞系，提取该细胞的总RNA，用RT-PCR检测CD基因的表达。

Methods:The total RNA was extracted from mouse RAW264.7 cells stimulated by LPS.The sequence including the whole length of HMGB1 was amplified by RT-PCR and inserted into pMD-19T.The combinant vector was used as a template for PCR which was cloned into vector pMD-19T,then subcloned into expression vector pET-26b with pelB signal sequence and His-Taq sequence.After transforming E.coli BL21(DE3) and four hours induction by IPTG,HMGB1 expression confirmed by SDS-PAGE and the purification was performed by Ni2+-chelate affinity chromatograph.

脂多糖刺激后的RAW264.7细胞，提取总RNA，经RT-PCR扩增出含HMGB1的目的片段，克隆于pMD-19T载体，再亚克隆至含有pelB引导肽及His-标签肽的高效表达载体pET-26b，转化大肠杆菌BL21（DE3），经IPTG诱导后行SDS-PAGE鉴定目标蛋白表达，用镍鳌合琼脂糖凝胶亲和层析法分离纯化含His-标签肽的目的蛋白。

Methods:The total RNA was extracted from mouse RAW264.7 cells stimulated by LPS.The sequence including the whole length of HMGB1 was amplified by RT-PCR and inserted into pMD-19T.The combinant vector was used as a template for PCR which was cloned into vector pMD-19T,then subcloned into expression vector pET-26b with pelB signal sequence and His-Taq sequence.

脂多糖刺激后的RAW264.7细胞，提取总RNA，经RT-PCR扩增出含HMGB1的目的片段，克隆于pMD-19T载体，再亚克隆至含有pelB引导肽及His-标签肽的高效表达载体pET-26b，转化大肠杆菌BL21（DE3），经IPTG诱导后行SDS-PAGE鉴定目标蛋白表达，用镍鳌合琼脂糖凝胶亲和层析法分离纯化含His-标签肽的目的蛋白。

It was found that Os-RLK1 constitutively expressed in vegetative tissues, such as root, stem and leaf, at mRNA and protein level by using RT-PCR and immuno-histochemistry analysis.

另外，在mRNA和蛋白质水平上分别利用RT-PCR和免疫组织化学法进行分析，结果表明Os-RLK1在水稻的根，茎和叶中都有组成性的。

However, as the name（read－only memory）implies, CD disks cannot be written onorchanged in any way.

然而，正如其名字所指出的那样，CD盘不能写，也不能用任何方式改变其内容。

Galvanizes steel pallet is mainly export which suits standard packing of European Union, the North America. galvanizes steel pallet is suitable to heavy rack. Pallet surface can design plate type, corrugated and the gap form, satisfies the different requirements.

镀锌钢托盘多用于出口，替代木托盘，免薰蒸，符合欧盟、北美各国对出口货物包装材料的法令要求；喷涂钢托盘适用于重载上货架之用，托盘表面根据需要制作成平板状、波纹状及间隔形式，满足不同的使用要求。