查询词典 RNase

Orange-spotted grouper is a benthic protogynous marine fish with sex inversion. It is one of the most important culturing fish in the southeast of Asia. As in other vertebrates, its growth is mainly controlled by growth hormone . Few data are available concerning the regulation of GH in grouper. In this paper, the regulation of growth hormone release from pituitary of orange-spotted grouper and its mRNA level was determined by radioimmunoassay and RNase protection assay.

中文题名斜带石斑鱼生长激素分泌及其基因表达调控的研究副题名外文题名 Regulation of growth hormone and its gene expression in orange-spotted grouper，Epinephelus coioides 论文作者冉雪琴导师林浩然学科专业水生生物学研究领域\研究方向学位级别博士学位授予单位中山大学学位授予日期2003 论文页码总数140页关键词生长激素基因表达石斑鱼馆藏号BSLW /2003 /Q959 /39 应用分子克隆技术，构建了斜带石斑鱼生长激素(Growth hormone，GH)基因重组质粒，在大肠杆菌细胞中获得高效表达，亲和层析纯化斜带石斑鱼GH融合蛋白做为抗原，免疫家兔制备特异性抗体，通过酶联免疫实验、放射免疫测定、离体脑垂体静态孵育和液相杂交／核糖核酸酶保护试验及化学发光检测技术，研究了斜带石斑鱼GH分泌及其mRNA表达的调控机制，对于斜带石斑鱼的生长、繁殖、人工养殖等具有重要意义。

All sequences were aligned with S genes in GeneBank by BLAST software.The result indicated that all 10 fragments contained domain encoding 5 high conservation regions(C1,C2,C3,RC4,C5) and hypervariable regionof rosaceous S-RNase.RHV and intron characterized S gene furthermore.

测序结果表明，这些DNA片段的核苷酸序列中都具有编码蔷薇科S-RNAase 5个高度保守区域：C1、C2、C3、RC4、C5的序列、编码高变区的序列以及变异度很大的内含子序列，并且其高变区和内含子序列具有扁桃S基因特异性。

METHODS: Porcine cornea stromata were processed by serial digestion of trypsin, DNase and RNase, and then were implanted into corneal stroma layer of New Zealand rabbits.

将用去垢剂联合酶的方法脱细胞处理的猪角膜基质材料植入兔角膜基质囊袋中，进行裂隙灯观察并照相。

Because different alleles of S-RNase are usually coded by the different amino acids in HV region, therefore,besides study on the genomic level, other approaches on the mRNA and protein level or cross-pollinated test are also important to confirm the S genotypes.

因此，除了在分子生物学的水平上进行研究外，其他方法如mRNA、蛋白质和杂交授粉等水平上的研究在确定S基因型上也同样重要。

L~(-1) each, 3.5μL of downstream primer 10μmol ? L~(-1,4μL of RNA,5μL of double-distilled DEPC H_2O;PCR mixture consisted of 20μL of RT-Mixture,5μL of 10 × PCR buffers, 1μL of Taq5U·μL~(-1, 5uL of mixture of dNTPs the 2.5 mmol ?

包括AMV5u·μL~(-11μL，5×Reverse transcriptase buffer 4μL，RNase Inhibitor40U·μL~(-10.5μL，dNTPs混合物10mmol·L~(-1each2μL，下游引物10μmol·L~(-13.5μL，模板4μL，水5μL；PCR反应体系：总反应体系为50μL。

In addition, activation and inactivation of p34cdc2are induced by Ca2+and prevented by Ca2+ chelators in permeabilized cells and cell - free systems.

细胞用胰蛋白酶N.2％）消化后，在 PBS中洗2次，1200 x g离心 10分钟去上清，加人 70％的冷乙醇，于一20t 30分钟。去除乙醇后，细胞中加人含有 RNase AN.lin牙IIl）的 PBS中放在 37 t培养箱 30分钟。

By using lysozyme, RNase A and protein disulfide isomerase as model proteins, we present a hypothesis that protein cross-linking can be accomplished in three concerted steps:(1) a change in protein conformation;(2) formation of interchain disulfide bonds; and (3) formation of interchain isopeptide cross-links.

我们以溶菌酶、核糖核酸酶A和蛋白质二硫键异构酶为模型对异肽键进行了体外详细的研究，提出了解释异肽键形成机理的假说：1蛋白质发生构象变化包括二级结构的改变；2蛋白质形成分子间二硫键：3二硫键的形成帮助蛋白质形成了分子间异肽键。

RNase P ribozymes for the studies and treatment of human cytomegalovirus infections［J］.

任何给定的RNA序列如果能够提供这些结构要求，均可作为M1RNA核酶的靶基因［7~9］。

Pure mitochondrial DNA was successfully obtained by using SDS and proteinase K to crack mitochon, phenol: chloroform: isoamyl alcohol extraction to remove protein, and digestion with RNase A.

用SDS和蛋白酶K裂解线粒体，经酚：氯仿：异戊醇抽提除去蛋白，并用RNaseA消化从而得到单纯线粒体DNA。

From the tiger frog virus genome, an open reading frame, whose deduced amino acid sequence shows identity with RNase Ⅲ,was identified.

对蛙病毒核糖核酸酶Ⅲ基因序列进行分析。

Breath, muscle contraction of the buttocks; arch body, as far as possible to hold his head, right leg straight towards the ceiling (peg-leg knee in order to avoid muscle tension).

呼气，收缩臀部肌肉；拱起身体，尽量抬起头来，右腿伸直朝向天花板（膝微屈，以避免肌肉紧张）。

The cost of moving grain food products was unchanged from May, but year over year are up 8%.

粮食产品的运输费用与5月份相比没有变化，但却比去年同期高8％。