查询词典 RNase

First, we determined the chromosomal location of the S locus in Antirrhinum by fluorescence in situ hybridization of metaphase chromosomes of a self-incompatible line of S〓S〓 using an S〓 RNase gene and its corresponding 63kb BAC clone as probes, respectively.

玄参科植物金鱼草的自交不亲和性和茄科和蔷薇科类似，都属配子体自交不亲和植物，它们S位点都编码一类控制花柱自交不亲和性的核酸酶，称为S RNase。

At first, RNase A was denatured by urea and dithiothreitol. Effect of strong denaturant urea and DTT on enzyme activity and free thiol number was investigated.

首先利用脲和DTT作为变性剂，研究了脲和DTT浓度对RNase A的酶活和自由巯基的影响规律。

The impacts of environmental factors, including temperature, pH, cation, denaturant and protease on the enzymatic activity of PEG ylated RNase, were investigated in detail.

研究了温度、pH、阳离子、变性剂、蛋白酶降解等环境因素对PEG化RNase酶活性的影响。

3 Effects of zinc levels on endogenous hormone and nitrogen metabolism in leaves of balsam pear The results showed that zinc application could increase IAA, GA〓, protein, nuclear contents and NRase activity, but decrease tryptophane, nitric nitrogen , free ammonic acids contents and RNase activity in leaves of plants.

1.3 锌对苦瓜叶片内源激素与氮代谢的影响结果表明：施锌可提高叶片吲哚乙酸、赤霉素、蛋白质、核酸含量以及硝酸还原酶活性；降低色氨酸、游离氨基酸和NO〓含量以及RNase活性。

Objective: To observe the targeting therapy of the hdsFv-RC-RNase recombinant single chain immunotoxin on the xenograft of the human hepatocellular carcinoma in nude mice and to explore its clinical potentiality.

目的：观察抗肝癌重组单链免疫毒素hdsFv-RC-RNase对荷人肝癌裸鼠移植瘤的导向治疗作用，并探讨其临床应用价值。

Thus it is presented an effective RNA extraction method based on the nuclease adsorbent macaloid, poly vinyl pyrrolidone, and high concentration of KAc and ethylene glycol monobutyl ether, which has successfully extracted high-quality RNA from many materials difficult to RNA isolation, such as RNase-rich rabbit liver, plant and microbial tissues rich in polysaccharides, lipids and polyphenol compounds.

现利用硅藻土对RNase的吸附性，结合PVP、高盐及乙二醇丁醚沉淀等处理，建立了一种广泛适用的RNA提取方法。在富含多糖的玉米胚乳，富含RNase的动物肝脏，多酚多油脂的银杏、麻疯树以及木霉、酵母等10多种RNA提取困难的动、植物与微生物材料中都提取出完整性好，得率高的RNA。

This report identified S-RNase genes of sweet cherry (Prunus avium L.), presented the sequences of S genes by using a pair of specific primers PruC2 and PruC4R based on the conserved regions C2 and RC4 of Rosaceous S-RNase genes and researched the S gene specific productsfrom the genomic DNAs of different cultivars in which most of the S genotypese were unknown.

根据蔷薇科S-RNase基因高度保守区C2 和RC4区设计一对特异引物PruC2 和PruC4R，对甜樱桃品种的基因组DNA进行S基因特异PCR扩增。

The degradation of pollen tube RNA in vitro by stylar S-RNase at different temperatures was performed to detect the extent of degradation using the agarose gel electrophoresis. The germination and tube growth of self-pollen and the RNA of self-pollen tube were inhibited and degraded significantly greater than cross-pollen respectively at 10℃ and 25℃, but no significant difference when the temperature rose to 35℃.

10℃、25℃时，花柱S-RNase抑制自花花粉萌发和花粉管生长的程度极显著大于抑制异花花粉的程度；花柱S-RNase降解自花花粉管RNA的程度也明显大于降解异花花粉管RNA的程度；温度升高到35℃时，自花和异花间花粉萌发和花粉管生长被花柱S-RNase抑制的程度不存在显著性差异；花柱S-RNase降解自花和异花花粉管RNA的程度也相似。

Adding purified stylar S-RNase from Prunus mume cultivar 'Gessekai' to liquid medium, for cultivating self- and cross-pollen at temperatures lot, 25℃ and 35℃, respectively, then determining the rate of pollen germination and the length of pollen tube after different periods of cultivating time.

在液体培养基中添加从果梅'月世界'花柱中纯化的S-RNase，并分别于10℃、25℃和35℃条件下培养自花、异花花粉，测定不同培养时间后的花粉萌发率和花粉管生长长度；离体条件下进行果梅花柱S-RNase降解花粉管RNA反应后，用琼脂糖凝胶电泳检测不同温度条件下自花、异花花粉管RNA被降解的程度。

Specific recognition and degradation of stylar S-RNase for self-pollen tube RNA, resulted in the stop of self-tube growth in Prunus mume. The recognition specificity of stylar S-RNase for the RNA weakened with the rise of temperature, consequently regulating the self-incompatibility reaction in Prunus mume.

果梅花柱S-RNase特异性识别并降解自花花粉管RNA，导致自花花粉管停止生长；随着温度的升高花柱S-RNase对花粉管RNA的识别特异性减弱，从而调节果梅的自交不亲和性反应。

Breath, muscle contraction of the buttocks; arch body, as far as possible to hold his head, right leg straight towards the ceiling (peg-leg knee in order to avoid muscle tension).

呼气，收缩臀部肌肉；拱起身体，尽量抬起头来，右腿伸直朝向天花板（膝微屈，以避免肌肉紧张）。

The cost of moving grain food products was unchanged from May, but year over year are up 8%.

粮食产品的运输费用与5月份相比没有变化，但却比去年同期高8％。