查询词典 Promoter

To investigate the role of gene Gli3 in idiopathic congenital talipes equinovarus, we constructed the Gli3 luciferase reporter gene expression vectors to analyze the promoter activity of the rat gene Gli3. The regulatory element in the promoter region of the rat Gli3 was predicted using P-Match software and further verified by ChIP experiment.

为了探讨人类GLI3基因在单纯性马蹄内翻足(Idiopathic congenital talipes equinovarus， ICTEV)发生时所起的作用，文章构建了大鼠Gli3基因启动子区域荧光素酶报告基因表达载体来分析Gli3基因启动子的活性，并利用P-Match软件预测大鼠Gli3基因启动子区域可能的调控元件，应用ChIP实验加以验证。

Ethylation specific PCR analysis also revealed that the BLU promoter was highlymethylated in 74％(17 of 23) of primary tumors where the gene was downregulated,whereas only 2 of 9 non-neoplastic nasopharyngeal epithelia exhibitedhypermethylation in the BLU promoter region.

SP分析的结果表明，在23例表达下调的鼻咽癌组织中，17例(74％，17／23)标本的BLU基因发生了异常甲基化，而9例非癌对照中仅有2例甲基化，说明启动子甲基化在BLU基因的转录失活中具有重要作用。

However, the mutation of GATA site at -2948bp remarkably reduced the reporter gene activity driven by SV40 promoter, but not by ε-globin gene proximal promoter. To test whether those sites act synergistically, we mutated 2 of these three binding sites with different combinations.

然而，GATAb（在-2948bp的GATA位点）的突变明显地降低了SV40启动子介导的报告基因转录活性，但是对ε-珠蛋白基因启动子介导的报告基因表达却影响不大。

The result indicates the length of the clone sequence is 963bp and shares a similarity of 45.24 % with the published Glycine max glycinin subunit G7 (Gy7) gene promoter.but have more similar to quantity and distance of promoter elements, they all contained typical TATA-box, CAAT-box and necessary regulatory motifs of seed-specific expression.

测序结果表明所克隆的片段长为963bp，与预期设计的大小一致，该片段与已发表的11S球蛋白基因启动子序列的相似性为45.2％，但所含的启动子序列元件的数量和距离上很相似，均具有有典型的TATA盒和CAAT盒，以及种子特异表达所必须的调控元件。

In the report, the maize ubiquitin 1 promoter Uhi, a high efficiency promoter in gramineous crop, had been introduced into the original plasmid with sgna gene.

利用在禾谷类作物中表达效率较高的启动子Ubi对含目的基因sgna的现有载体进行了改造。

While AcMNPV and BmNPV had no stimulatory effect in nonsensitive cell lines BmNPV hr3 has been demonstrated to increase the transcriptional activity of cislinked hel510 promoter. In this study, Sf-21, Bm-N and Hi-5 cell lines were transfected with pBmhel510luc-hr3down, and then were infected with each of AcMNPV, BmNPV and HyNPV. The results indicated that viral factors could interact with hr3 to significantly copromote the transcriptional level of hel510 promoter.

BmNPV hr3已被证明具有增强子功能，将hr3与hel510启动子顺式连接的质粒pBmhel510luc-hr3down转染Sf-21、Bm-N和Hi-5细胞后，再用上述三种病毒分别感染每一种细胞，hel510启动子的转录活性得到显著提高，表明病毒因子能够识别hr3，二者通过相互作用，共同增强解旋酶基因启动子的转录水平。

PPARα ligands suppressed OPN promoter actiity, and an actiator protein-1 consensus site conferred this repression. Oerexpression of c-Fos and c-Jun reersed the inhibitory effect of PPARα ligands on OPN transcription, and, in chromatin immunoprecipitation assays, PPARα ligands inhibited c-Fos and phospho–c-Jun binding to the OPN promoter.

PPARα配体可抑制OPN启动子活性，一个激活蛋白-1的一致序列位点与这种抑制有关。c-Fos和c-Jun的过表达可逆转 PPARα配体对OPN转录的抑制作用，并且在免疫沉淀实验中，PPARα配体可抑制c-Fos及磷酸化c-Jun与OPN启动子的结合。

First of all, the cytokeratin 19 promoter segment was cloned from hepatocellular carcinoma cell line HepG2 and then following the promoter a renilla luciferase and a red fluorescence protein's fusion gene were inserted to finish the double report lentiviral vector.

首先，通过PCR方法从肝癌细胞系HepG2的全基因组中克隆了CK19启动子片段，构建了CK19启动子调控的海肾荧光素酶和红色荧光蛋白（red fluorescent protein，RFP）双报告载体（pSicoR-CK19-hrl-mrfp）。

Transcriptional activity of ACL recombinants normalized by Renilla was significant difference with pGL3-Basic expect for construct -27 and -15 (P＜0.01). Construct -919 contains highest activity. 3 times reduction of transcriptional activity from-919 to -679bp indicated that negative regulation factors located probably in this region. The activity started on construct -73 suggested the basal promoter activity was located within the -73 to +77bp region; Transcriptional activity of IDHβrecombinants was not significantly different between the recombinant -58 and pGL3-Basic. The activity started on construct -82, decreasing with the length of the fragment up to -164 in despite of a bit of fluctuation, and kept increasing from construct -164 up to -279. Thus, the basal promoter activity was located within the -82 to +16bp region, whereas the upstream 197bp conferred maximal transcriptional activity.

采用5'端缺失策略，结合启动子预测结果，分别构建了8个ACL基因和19个IDH3β基因启动子区重组子，将其转染猪PK15细胞系，并利用荧光素酶双报告基因系统检测了它们的活性，结果表明：在所构建的猪ACL基因5侧翼序列的8个重组子中，除pGL-ACL27和pGL-ACL15外，其它重组子的荧光素酶活性与阴性对照均达到极显著水平(P＜0.01)，表明它们均具有启动子活性，pGL-ACL919活性最强，到pGL-ACL679活性下降了将近3倍，说明从-919bp到-679bp区域可能存在负的调控位点，从pGL-ACL621到pGL-ACL73活性有小幅度下降，到pGL-ACL27活性降到与阴性对照水平无显著差异，表明维持该启动子的基本活性区域位于-73bp到+77bp之间；IDH3β基因启动子活性开始于pGL-IB82，然后虽然有些波动，但是活性一直下降直到-164bp处，之后活性又开始升高，直到-279bp处活性达到最高，这些表明维持该启动子的基本活性区域位于-82bp到+16bp之间，从-82到-279之间的179bp区域具有最高的启动子活性。

Transcriptional factors play an equivalent role in plant during drought adaptation and cold acclimation. In order to study the expression pattern of CB F4 gene promoter and explore its potential application in plant genetic engineer ing, CBF4 promoter fragment was amplified by PCR method from Arabidopsis thalian a genome DNA.

根据已有文献及公开的拟南芥基因组序列，利用PCR方法从拟南芥基因组DNA中扩增得到了转录因子CBF4上游的DNA片断，对其进行序列分析表明与GenBank序列高度同源，同时对其进行初步的功能分析。

The split between the two groups can hardly be papered over.

这两个团体间的分歧难以掩饰。

This approach not only encourages a greater number of responses, but minimizes the likelihood of stale groupthink.

这种做法不仅鼓励了更多的反应，而且减少跟风的可能性。