查询词典 Promoter

Based on above experiments, I prepared transgenic tobacco plants harboring the expression cassette of the GUS gene controlled by the Hsp70 gene promoter. In the mean time, I also expressed in bacteria and purified the proteins encoded by the P1 and NIa cistrons of PSbMV.

在此基础上，我们又培育了含Hsp70基因启动子指导下的GUS基因的表达盒的转基因烟草，并在细菌细胞中表达和提纯了PSbMV P1、NIa的蛋白质。

PPARs can regulate target genes expression by binding to sequence-specific PPAR response dements （peroxisome proliferator responsive element,PPRE） in the promoter regions of target genes.

通过与位于某些基因上游的特异DNA反应元件（peroxisome proliferator responsive element，PPRE）相互作用而调控靶基因的表达。

In the ring after the fight, his promoter, Bob Arum, threw out three possibilities: John Duddy, Oscar De La Hoya and Manny Pacquiao, if he beats Ricky Hatton on May 2, although Arum acknowledged that the weight difference could pose a problem.

赛后，在拳台上，他的推广人给出了小查的三位可能的对手：约翰。达迪、霍亚和帕奎奥（如果他能在5月2日胜了哈顿），同时，阿鲁姆也承认体重级别的差异也是个问题。

In addition to promoter regions, transcription of most eukaryotic genes is regulated by other dna sequences called enhancers.

除了启动子区域外，大多数真核基因的转录还受到另一段被称为增强子的dna序列的调控。

This chapter covers database resources such as NCBI/ Entrez, Ensembl and UCSC as well as analytical tools for sequence alignment, promoter analysis and molecular interactions.

本章覆盖了数据库资源，例如NCBI/Entrez，Ensembl和UCSC，以及序列比对、启动子分析和分子相互作用的分析工具。

Full length LMP2A cDNA was firstly incised from pGEM-T-LMP2A with EcoR Ⅰ, Sma Ⅰ digestion, and then inserted into eucaryote. expression plasmid pCIcc controlled by CMV promoter. The CMV-LMP2A-SV40 expression unit was digested by ClaI, and inserted into E1-substituted adenovirus vector pAx1cw. Then the LMP2A recombinant adenovirus vector was cotransfected into 293 cells together with EcoT22I digested Ad5-TPC.

将带有LMP2A cDNA的重组质粒pGEM-T-LMP2A用EcoR Ⅰ、Sma Ⅰ双酶切下LMP2A cDNA，并将其插入含同样酶切位点的真核表达质粒pCIcc中，使其受控于CMV启动子下；用ClaI切下CMV-LMP2A-SV40表达单元，插入E1、E3区替代的腺病毒载体pAX1CW，选择正确的克隆pAX1CW-LMP2A与Ad5 DNA-末端肽复合体共转染293细胞，通过同源重组获得复制缺陷型的重组腺病毒(Ad5-LMP2A)。

The eucaryotic cell expression vector modulated by HPSE gene core promoter could be successfully constructed, which could express specifically in tumor cell lines, and its activity should be further enhanced.

成功构建了由HPSE核心启动子驱动的真核细胞表达载体，该载体可在肿瘤细胞特异性表达，但其活性需进一步加强。

The shRNA eukaryon expression plasmid vectors targeting rat Pik3cb were constructed using VSMC-specific SM22α promoter.

采用血管平滑肌细胞特异性SM22α启动子构建靶向大鼠Pik3cb基因的干扰RNA真核表达载体，既可抑制新生内膜增生，又可防治血栓的形成。

The results of this work demonstrated that a DNA autonomous replication region with the promoter function in yeast was firstly identified, which is significant to research on the mechanism of replication and transcription in eukaryote.

以上结果首次发现了一个兼有启动子功能的酵母DNA自主复制起始区，这对于研究真核DNA复制与转录机制及两者之间的关系有重要的意义。

It was demonstrated that the Vp54 gene promoter has activity in both prokaryotes and eukaryotes.

这说明Vp54基因的启动子在原核和真核生物中均具有启动活性。

The split between the two groups can hardly be papered over.

这两个团体间的分歧难以掩饰。

This approach not only encourages a greater number of responses, but minimizes the likelihood of stale groupthink.

这种做法不仅鼓励了更多的反应，而且减少跟风的可能性。