查询词典 Promoter

In this paper, the fib-H promoter was choned by PCR method and sequenced. Sequence analysis showed that the cloned fib-H promoter harbored the typical motifs, which resulted in the gene expressing in the silk gland specifically.

为利用其时空调控机制，通过PCR扩增方法克隆fib-H启动子片段，并进行序列分析，进而构建由fib-H启动子控制报告基因DsRed的重组载体pSK-FH-DsRed-PolyA，通过家蚕BmN细胞和丝腺进行瞬时表达。

Rice Tungro bacilliform virus promoter is a phloem specific promoter.

水稻Tungro病毒启动子是一个只在韧皮部表达的启动子。

The two disease-resistance genes chitinase and glucanase,which were respectively directed by commelina yellow mottle virus promoter and CaMV35S promoter,were introduced into cotton genome via Agrobacterium tumefaciens .

通过根癌杆菌介导法，将维管束特异启动子(竹节花黄斑病毒启动子CoYMV)启动的几丁质酶和CaMV3 5S启动的β 1，3 葡聚糖酶嵌合双价基因，导入陆地棉品种冀合 3 2 1和中棉所 3 5。

Accordingly, a new plasmid Cm1301 was constructed containing osGP fragment (-197～-56) from the osRACD promoter with the minimal promoter,-90 region of CaMV 35S which confers Gus activity detectably only in roots. In the transgenic plants containing Cm1301, GUS activity was detected mostly in the anther.

由于35S基本启动子是一个根特异表达的启动子，因此将包含osDP5区段的osGP片段（-197～-56）与35S基本启动子连接，构成植物表达载体Cm-1301并转化烟草，研究发现，osGP区段使gus基因在雄蕊中表达水平显著提高。

Objective To construct a hTERT and Cox-2 promoter regulated oncolytic virus by replacing the endogenous adenovirus Ela and Elb promoter,respectively.

目的 应用同源重组的方法构建以hTERT和Cox-2启动子调控增殖的肿瘤特异性增殖腺病毒。

To investigate the role of gene Gli3 in idiopathic congenital talipes equinovarus, we constructed the Gli3 luciferase reporter gene expression vectors to analyze the promoter activity of the rat gene Gli3. The regulatory element in the promoter region of the rat Gli3 was predicted using P-Match software and further verified by ChIP experiment.

为了探讨人类GLI3基因在单纯性马蹄内翻足(Idiopathic congenital talipes equinovarus， ICTEV)发生时所起的作用，文章构建了大鼠Gli3基因启动子区域荧光素酶报告基因表达载体来分析Gli3基因启动子的活性，并利用P-Match软件预测大鼠Gli3基因启动子区域可能的调控元件，应用ChIP实验加以验证。

Ethylation specific PCR analysis also revealed that the BLU promoter was highlymethylated in 74％(17 of 23) of primary tumors where the gene was downregulated,whereas only 2 of 9 non-neoplastic nasopharyngeal epithelia exhibitedhypermethylation in the BLU promoter region.

SP分析的结果表明，在23例表达下调的鼻咽癌组织中，17例(74％，17／23)标本的BLU基因发生了异常甲基化，而9例非癌对照中仅有2例甲基化，说明启动子甲基化在BLU基因的转录失活中具有重要作用。

However, the mutation of GATA site at -2948bp remarkably reduced the reporter gene activity driven by SV40 promoter, but not by ε-globin gene proximal promoter. To test whether those sites act synergistically, we mutated 2 of these three binding sites with different combinations.

然而，GATAb（在-2948bp的GATA位点）的突变明显地降低了SV40启动子介导的报告基因转录活性，但是对ε-珠蛋白基因启动子介导的报告基因表达却影响不大。

The result indicates the length of the clone sequence is 963bp and shares a similarity of 45.24 % with the published Glycine max glycinin subunit G7 (Gy7) gene promoter.but have more similar to quantity and distance of promoter elements, they all contained typical TATA-box, CAAT-box and necessary regulatory motifs of seed-specific expression.

测序结果表明所克隆的片段长为963bp，与预期设计的大小一致，该片段与已发表的11S球蛋白基因启动子序列的相似性为45.2％，但所含的启动子序列元件的数量和距离上很相似，均具有有典型的TATA盒和CAAT盒，以及种子特异表达所必须的调控元件。

In the report, the maize ubiquitin 1 promoter Uhi, a high efficiency promoter in gramineous crop, had been introduced into the original plasmid with sgna gene.

利用在禾谷类作物中表达效率较高的启动子Ubi对含目的基因sgna的现有载体进行了改造。

"Yes, now you can give yourself airs," she said, you have got what you wanted.

"对了，您现在高兴了，"她说道，这是您所期待的。

Then the LORD said to me: Rebel Israel is inwardly more just than traitorous Judah.

上主于是对我说：＂失节的以色列比失信的犹大，更显得正义。