查询词典 Promoter

Cerevisiae GAPp, suggesting that H. polymorpha △9-fatty acid desaturase was compatible with desaturation system of S. cerevisiae whereas the promoter of H-OLE1 gene had no activity in heterologous cells.

说明H-OLE1基因编码△9-脂肪酸去饱和酶，多形汉逊氏酵母的△9-脂肪酸去饱和酶和酿酒酵母的脂肪酸脱饱和系统相亲和，而H-OLE1基因的启动子在异源细胞中没有活性。

Polymorpha cells in low level and produced a 1. 5kb transcript. Transcription of the gene was slightly repressed by exogenous △9 UFA. The FAR element in S. cerevisiae OLE1 promoter described by Choi et al. might not be crucial for positive transcriptional regulation of H-OLE1 gene.

Northern杂交发现，H-OLE1基因在细胞中以较低水平表达，产生1.5kb的转录子；基因表达略受不饱和脂肪酸的抑制；在多形汉逊氏酵母H-OLE1基因的转录调节中，Choi等在酿酒酵母OLE1基因中发现的脂肪酸调节元件FAR可能不是关键的。

The fusion gene was inserted downstream to the MOX promoter of Hansenula polymorpha shuttle plasmid pDGXHP2.0, and the constructed multicopy secretory recombinant plasmid was transformed to Hansenula polymorpha ATCC34438 Ura3(superscript - by electroporation.

将融合基因插入汉逊酵母穿梭质粒pDGXHP2.0的甲醇氧化酶基因启动子下游，构建成多拷贝分泌型重组表达载体。

To play the political key role of state-owned enterprise Party organization is the scientific conclusion that the third generation leading group of the Party Central Committee draws on the basis of summarizing the historical experiences in construction、 reform and development of state-owned enterprise since founding the PRC especially since reform and opening to the world, and is the inevitable requirement for state-owned enterprise leading Party organization to become the organizer、 promoter and practician...

发挥国有企业党组织政治核心作用是党中央第三代领导集体总结建国以来尤其是改革开放以来国有企业建设、改革、发展的历史经验得出的科学结论，是国有企业党组织成为贯彻&三个代表&重要思想的组织者、推动者、实践者及建立中国特色现代企业制度的必然要求。十六大《党章》对国有企业党组织政治核心作用的主要体现做出了准确的表述。

Missense mutation of Tyr486Asp in Exon 5 of UGT1A1 gene is the pathogenic factor of the CNⅡ case in the family and recessively inherited. The mutation has no influence on the response elements of phenibarbital in the promoter region.

Tyr486Asp突变是本例CN-Ⅱ家系的致病性基因，该突变不影响UGT1A1基因启动子区苯巴比妥酸的应答元件，其遗传规律符合常染色体隐性遗传。

The tac promoter is now one of the most popular promoters used for gene expression. The Ptac-based expression system was regulated by the lac represser protein which is a product of lad gene.

Ptac启动子是研究基因表达最常用的启动子之一，在Ptac启动子基础上建立的表达系统受lacI基因编码的阻遏蛋白调控。

We further expressed the β-galactosidase reporter gene in R. ruber driven by promoter PlacZ. Results indicated that lacZ was an appropriate reporter gene for genetic or metabolic engineering research of Rhodococcus.

采用PlacZ启动子在红球菌宿主中启动b-半乳糖苷酶报告基因，结果表明， lacZ能够在红球菌中高效表达，是优选的红球菌报告基因。

The late phase expression cassette suited for adenovirus vectors was also constructed. The HBV S gene, HBV preS2+S gene and the late phase expression cassette +HBV S genes were respectively cloned into the Ad5 vector downstream of the E3 promoter. Semiconfluent monolayers of 293 cells were cotransfected with the above constructed plasmids and Ad5 DNA EcoRI A fragment. The progeny adenoviruses named rAd5S, rAd5MS, rAd5S2S were harvested.

通过共转染优化实验，建立了脂质体转染腺病毒的有效方法，并构建了适合腺病毒载体的晚期表达盒，进一步将乙型肝炎表面抗原基因、HBV preS2+S、腺病毒晚期表达盒+HBV S基因插入E3区，与EcoRI消化的Ad5 DNA A片段共转染细胞，并获得重组腺病毒，相应命名为：rAd5S、rAd5MS、rAd5S2S。

So it can be essential momitoringindex of colon carcinoma and its tumor specificness is apply by manygene therapy of tumor. Transcriptional regulatory element of CEAcontains enhancer element and promotor in the 5th flanking region whichcan control expression of objective gene in positive cancer tissue of CEA. Objectives: To explore the joint promoter with high activity,consisting CEA promotor and CMV enhanser.

目的：本课题探讨由CEA启动子、CMV增强子组成的高活性嵌合启动子，通过融合启动子的调控提高融合自杀基因yCDglyTK在靶细胞表达的特异性；并以无毒副作用生物相容性好的磷酸钙纳米为载体，将该自杀基因靶向导入结肠癌细胞中，以达到提高基因治疗靶向性及安全性的目的。

The open reading frame of Spinacia oleracea Betaine Aldehyde Dehydrogenase was retrieved from Spinacia oleracea and inserted into the Agrobacterium tumefaciens binary vector pBin438, which was driven by CaMV35S promoter, and produced the new binary vector pBSB. A. tumefaciens LBA4404 carrying this plasmid was used in genetic transformation of plants. Forty-five primary transgenic plants were detected by PCR and verified by the Southern blotting from 65 regenerated plants, of which 27 transgenic plants had only one copy of T-DNA.

摘 要：分离出菠菜甜菜碱醛脱氢酶基因构建成由CaMV35S驱动的双元植物表达载体pBSB，农杆菌菌株LBA4404携带该载体转化棉花，获得转基因棉花植株。65株转基因植株经过PCR筛选、Southern blotting分析证明有45株为成功的转化株，外源基因已经被整合到棉花的染色体组中，并以单拷贝插入居多。

The split between the two groups can hardly be papered over.

这两个团体间的分歧难以掩饰。

This approach not only encourages a greater number of responses, but minimizes the likelihood of stale groupthink.

这种做法不仅鼓励了更多的反应，而且减少跟风的可能性。