查询词典 Promoter

The hypermethylation of 5 CpG in promoter region serves as a mechanism of the inactivation of RASSF1A gene. This mechanism has been widely confirmed in multiple tumorgenesis including prostate carcinoma cell line DU145. Object Our study is to disclose the epigenic mechanism of the carcinogenesis of prostate cancer. Including: 1. We use demethylation agents in combination with chemotherapeutic drugs, to disclose whether demethylation agents can enhance the anticancer effect of chemotherapeutic drugs. 2. to study whether demethylation drugs can affect the methylation level of RASSF1A gene in.

目的 本研究旨在探讨前列腺癌的表遗传学发生发展机制，包括： 1、甲基化抑制剂与多种化疗药物联合应用，探讨二者间的协同作用，增强化疗药物疗效； 2、体外应用甲基化抑制剂对前列腺癌细胞DU145的RASSF1A基因甲基化水平的影响； 3、体外应用甲基化抑制剂对前列腺癌细胞DU145的RASSF1A基因及蛋白表达的影响，以期为进一步深入探索通过改变抑癌基因的甲基化而为前列腺癌发生机制和基因治疗提供有意义的数据和理论依据。

In this paper, an escherichia coli-saccharopolyspora erythraea shuttle vector containing the erya promoter region was constructed using the enhanced green fluorescent protein gene as a reporter. the shuttle plasmid was transformed into sac.erythraea a226 and streptomyces lividans jt46 by peg mediation, respectively. fluorescence microscopy confirmed that the egfp was expressed in both strains.

本文克隆了erya基因的启动子perya，以绿色荧光蛋白基因为报告基因，构建了大肠埃希菌-糖多孢红霉菌穿梭型质粒。peg介导原生质体转化法将穿梭型质粒分别转入糖多孢红霉菌a226与变铅青链霉菌jt46，荧光显微镜检测发现，此启动子在两菌株中都具有功能。

As most Streptomyces promoters are invalid in S. erythraea, the promoter of erythromycin-resistant gene, ermE, and Streptomyces chromosomic integration site, attP, and apramycin-resistant gene, apr from plasmid pSET152 are utilized to construct the expression vector pZMW.

因大多数链霉菌质粒的启动子在糖多孢红霉菌中都不能发挥作用，故利用糖多孢红霉菌染色体上红霉素抗性基因PermE启动子作表达载体启动子，并利用pSET152质粒上链霉菌染色体整合位点和氨朴霉素抗性基因，构建了表达载体pZMW。

The multi-copy expression vector pZM is a shuttle plasmid which can replicate in Escherichia coli, Streptomyces and S. erythraea. It contains PermE promoter, fd terminator, multiple clone site, thiostrepton and ampicillin resistance genes, and ColE1 ori and pJV1 ori replicons which make the vector replicate in E.

游离型多拷贝表达载体pZM是可以在大肠杆菌、链霉菌和糖多孢红霉菌中扩增的穿梭型质粒，带有PermE启动子、fd终止子、多克隆位点、硫链丝菌肽和氨苄青霉素抗性基因以及在大肠杆菌和糖多孢红霉菌中复制的ColE1 ori和pJV1 ori复制子，载体大小9880bp。

Recombinant CD 59 protein using ICAM-2 promoter with enhancer can be exlSt:essed highly, effectively and specifically in PAECs and transgenic mice.

以人细胞间黏附因子Ⅱ(ICAM-2)为启动子并带增强子的CD59重组基因在猪的血管内皮细胞及小鼠体内均高效特异表达。

The expression of BjCHI1 was highly inducible by methyl jasmonate treatment, wounding, caterpillar feeding, and pathogenic fugal infection. These observations suggest that the promoter of BjCHI1 gene might contain specific cis-acting elements for stress responses.

BjCHI1基因受伤害、茉莉酸甲酯、虫食、真菌侵染等的强烈诱导表达，这暗示BjCHI1启动子可能含有相应的诱导元件。

The THFA yield and selectivity were 2.0%higher than those by using catalyst Ⅰ without promoter,3.0%higher than those by using Raney nickel catalyst under the same reaction conditions and furfuryl alcohol conversion.

在相同反应条件和糠醇转化率条件下，四氢糠醇收率和选择性均比没有助剂的镍催化剂Ⅰ高2.0%，比骨架镍催化剂高3.0%。

The XYN7 gene, located on multicopy shuttle vectors pYES6/CT, was successfully expressed in the yeast Saccharomyces cerevisiae under the control of the GALI promoter and CYC1 transcription termination signal , respectively.

XYN7基因构建在多拷贝穿梭载体pYES6／CT上，在GALI启动子和CYC1转录终止信号的控制下成功在酿酒酵母中表达。

Takeshi Nagasaka, M.D., of the Department of Gastroenterological Surgery and Surgical Oncology at the Okayama University of Graduate School of Medicine, and colleagues analyzed methylation of the RAS腾讯QQ2 and 腾讯QQRP2 gene promoter腾讯QQrom 788 primary gastric and colorectal tissue specimens to determine whether methylation patterns could act as stage-dependent biomarkers of gastrointestinal tumorigenesis.

Takeshi Nagasaka医学博士来自冈山大学医学研究所胃肠外科和肿瘤外科部门，他和同事一起检测了788例早期胃癌和结直肠癌病人的组织样本中的RAS腾讯QQ2和腾讯QQRP2两个基因启动因子的甲基化现象。以确定甲基化模式可否作为胃肠道肿瘤发生的阶段性生物标记物。

The wheat germin gene was cloned by PCR and a plant expression vector was constructed harboring the germin gene drived by CaMV 35S promoter. Germin gene has been transferred into the tabacco plants.

本研究通过PCR扩增克隆了小麦germin基因，构建了35S启动子驱动的germin基因植物表达载体，经遗传转化获得了转基因烟草植株。

The split between the two groups can hardly be papered over.

这两个团体间的分歧难以掩饰。

This approach not only encourages a greater number of responses, but minimizes the likelihood of stale groupthink.

这种做法不仅鼓励了更多的反应，而且减少跟风的可能性。