查询词典 Promoter

Aim: To establish a hypoxia model of human umbilical vein endothelial cells-12 (HUVEC-12) with stimulating of Cobaltous chloride in vitro, and investigate activity of human endothelial nitric-oxide synthase promoter.

目的：建立氯化钴诱导的人脐静脉血管内皮细胞－12(HUVEC-12)缺氧模型，研究人血管内皮细胞一氧化氮合酶基因的启动子活性变化。

Methods: The cells were treated with the different concentrations of Cobaltous chloride. The lactic acid dehydrogenase from the supernatant of the cells was detected by a biochemical analysis. The cells were co-transfected with a pGL2-eNOS-p vector while stimulated with Cobaltous chloride at the different concentrations and disposing time, and the transcription activity of human eNOS promoter was determined through using a double luciferase reporter gene system.

用含不同浓度氯化钴的培养基培养细胞，检测细胞培养上清中的乳酸脱氢酶含量；将已构建的pGL2-eNOS-p质粒转染HUVEC-12细胞，利用双荧光素酶报告基因技术检测在不同浓度氯化钴和不同作用时间下的eNOS启动子转录活性。

The two disease-resistance genes chitinase and glucanase,which were respectively directed by commelina yellow mottlevirus promoterand CaMV35S promoter,were introduced into cotton genome via Agrobacterium tumefaciens.

通过根癌杆菌介导法，将维管束特异启动子(竹节花黄斑病毒启动子CoYMV)启动的几丁质酶和CaMV35S启动的β-1，3-葡聚糖酶嵌合双价基因，导入陆地棉品种冀合321和中棉所35。

Confronted with the new course reform, the teacher is not to turn his role into other roles, but to determine anew in accordance with the requirements of new course his own professional connotations, which are expressed in the following "5-er/or＇ s": The teacher is a service performer of the students, which is the essential characteristic of the role, showing the nature and the working attitude of the teacher; he is a promoter of the students＇study, which is the kernel characteristic of the role, normalizing his teaching behavior; he is a cooperator of teaching and study, which is the basic characteristic of the role, normalizing the equal relation between the teacher and students as well as between teaching and study ; he is a constructer of the new course and a teaching - study researcher, which are the significant characteristics of the role, normalizing his specialty research and advanced learning and improvement.

面对新课程改革，教师不是把自己的角色转变为其他角色，而是根据新课程的要求重新确定自己的职业内涵：教师是学生的服务者，这是教师角色的本质特征，表明教师的性质和工作态度；是学生学习的促进者，这是教师角色的核心特征，规范教师的教学行为；是教与学的合作者，这是教师角色的基本特征，规范师生平等的教学关系；是新课程的建设者和教学研究者，这是教师角色的重要特征，规范教师的专业研究与进修提高。

To elucidate whether Era is related to the death of bacterial cells expressed YggG294, A double promoter expression vector that can express YggG294 and Era proteins controllably in cells was constructed.

yggG是从大肠杆菌全基因组文库中钓取并克隆的Era结合蛋白基因，研究表明该基因可能表达产物YggG294蛋白对宿主菌的生长具有强烈的抑制作用。

ObjectiveTo study the effects of azone on the percutaneous of berberine hydrochloride in vitro so as to provide experimental basis for complex prescription Coptis chinensis plastics to choose absorb promoter and control production quality.

目的研究氮酮对盐酸小檗碱的体外透皮吸收的影响，为黄连复方涂膜剂透膜吸收促进剂的选择和生产质量控制提供依据。

To clone promoter-active fragments from Corynebacterium glutamicum for further construction of expression vectors.

获得谷氨酸棒杆菌10147基因组中具有启动子活性片段的结构序列，为构建表达载体做准备。

Moreover, 70% of core promoter regions of RR cuticular protein genes share the common TATAAA sequence by searching the online server TFSEARCH.

采用TFSEARCH等在线服务软件分析发现，在70%含有RR基序的家蚕表皮蛋白基因的核心启动子区具有通用的TATAAA序列。

Long-distance PCR was used to amplify the β-actin gene for black carp based on the β-actin gene sequences of Cyprinidae. Construct of EGFP expression system containing 5'UTR of β-actin gene for black carp was transferred into zygotes of mud loach and COS-7 cells and the results showed that 5'UTR of β-actin gene for black carp could be used as the promoter of the"all-fish"transgene.

基于鲤科鱼类Beta-肌动蛋白基因序列，采用长片段PCR技术，克隆了青鱼β-actin基因DNA片段，并构建了青鱼β-actin基因5'启动调控区绿色荧光蛋白表达载体，通过显微注射泥鳅胚胎和COS-7细胞转染实验，证明了所克隆的青鱼β-actin基因5'启动调控区具有启动调控功能，并用之来构建&全鱼&基因的启动子部分。

Contrast experiments showed that the "water type trajectory" would be obtained with the promoter of water structure, and "pyridine type trajectory" would be obtained with damager of water structure. They would be related to the strength of electromagnetic field radiation in natural surroundings.

对比 实验表明产生这两类导电轨迹的原因是由于物质对水的结构有促进能力和破坏能力两种不同功能所造成；而电导率持续变化的原因则可能与自然环境某些电磁辐射场强度的持续变化有关连。

The split between the two groups can hardly be papered over.

这两个团体间的分歧难以掩饰。

This approach not only encourages a greater number of responses, but minimizes the likelihood of stale groupthink.

这种做法不仅鼓励了更多的反应，而且减少跟风的可能性。