查询词典 Promoter

In this study, what had been used inculding CBF1 and CBF3 of CBF family, its downstream COR15a gene , regulating element CRT/DRE in promoter of COR15a gene, Cloning CBF1 and CBF3 from Arabidopsis thaliana, and Cloning ApCOR15a gene from Arabis pumila of xinjiang. On the basis of plasmid pBI121,being used CaMV35S promoter and SAR iductivity promoter synthesized by PCR, six plant expression vectors had been constructed: pCB111, pCB111-1, pCB112,pCB112-1,pCB113, pCB113-1, and then transformed to Agrobacterium GV3101 by electics.

本研究选用了CaMV35S组成型启动子和人工合成的由水杨酸诱导的启动子SAR、拟南芥CBFs家族中的CBF1和CBF3、新疆小拟南芥COR15a基因及其CRT/DRE顺式作用元件构建了不同组合的六个植物表达载体：pCB111、pCB111-1、pCB112、pCB112-1、pCB113和pCB113-1，并采用农杆菌介导的叶盘转化法转化烟草，获得了大量的抗性植株。

The effects of catalyst, catalyst promoter, ratio and amount of catalyst and catalyst promoter, temperature, reaction time and pressure on the selectivity and yield of benzaldehyde have been investigated.

重点考察了催化剂种类、助催化剂种类、催化剂和助催化剂的配比和用量、反应温度、反应时间和反应压强等因素对苯甲醛的选择性和收率的影响。

New strategies for breeding seedless plants have been proposed by using these genes. These include expression of some cytotoxin genes or genes for production of hormones in plants under the control of seed_coat_specific promoter or ovary_specific promoter and "terminator technology" etc. It's clear that these transgenic methods will be introduced into a wider range of fruit and vegetables in the future.

应用这些目的基因，已建立了转基因生产无籽果实的新策略，如在种皮或子房特异性启动子控制下的生长素基因或细胞毒素基因的表达及"终止子"技术的运用等，这将大规模地促进蔬菜和水果生产，提高果蔬产品的市场价值。

Mutation of the gARID1 binding site in the cwp1 promoter resulted in a decrease of promoter activity and mRNA levels during vegetative growth and encystation, suggesting that the gARID1 binding sites are positive cis-acting elements of the cwp1 gene promoters in both trophozoites and encysting cells. Using cotransfection assay, we also found overexpession of gARID1 can increase luciferase activity and mRNA levels, while mutation of the gARID1 binding sites of the cwp1 promoter resulted loss of this transactivation.

我们也用萤光酵素基因接上cwp1基因启动子，如果将其cwp1启动子上的gARID1结合位置突变，也可以发现萤光酵素和讯息RNA的表现有明显的下；再用cotransfection assay，可发现gARID1会使萤光酵素和讯息RNA表现明显上升，然而如果将其cwp1启动子上的gARID1结合位置突变，这种转活化则消失。

Firstly, the pGL3-Control vector was reconstructed , the pGL3-Enhancer vector deletious of SV40 promoter gene was obtained . Then the IFN-β promoter gene was cloned into the pGL3-Enhancer vector and pGL-IP21, the Luciferase reporter plasmid with IFN-β promoter was OK. The availability of pGL-IP21 was verified by NDV ,the inductor of IFN-β, the Luciferase activity was assayed in cells transfected with pGL-IP21 by Luminometer.

首先将pGL3-Control载体进行了改构，除去了SV40启动子基因，获得了pGL3-Enhancer载体，将获得的IFN-β启动子基因连入载体中，构建了带有人IFN-β启动子基因的荧光素酶报告质粒IP-21，并且通过实验证明所构建的质粒在干扰素的诱导剂NDV的作用下能够表达荧光素酶活性，照度计检测荧光素酶活性增强，从而验证了所构建的重组质粒的有效性。

To prove that the cloned DNA fragment can express tryptopanase,a new plasmid pET28C-TnaA , in which the cloned DNA fragment was located downstream of T7 promoter on pET28c was constructed and transformed into host BL21(DE3),a BL21 lysogen of bacteriophage DE3 in which the only promoter known to direct transcription of the T7 RNA polymerase gene is the lacuvS promoter ,which is inducible by IPTG.

为了证明质粒上的基因能表达出有活性的色氨酸酶，将这个DNA片段克隆到PET28c质粒的BamHI和HindⅢ位点上，使该片段受T7 RNA聚合酶的启动子控制，然后转化噬菌体DE3的溶源菌BL21(DE3)。

Here, we inserted the RRD3 fragment into the plant promoter-capture vector, pCAMBIA1391Z, and examined whether the RRD3 fragment has promoter activity in plants. Transgenic tobacco and rice calli both showed β-glucuronidase activity, indicating that RRD3 can act as a promoter in both monocot and dicot plants.

我们将RRD3片段插入到植物启动子捕获载体pCAMBIA1391Z中，检测RRD3片段是否在植物中也有启动子活性，转基因烟草再生植株和水稻愈伤组织均显示了gusA基因的表达，表明其在单子叶和双子叶植物中均可行使启动子功能。

Methods: The human KDR promoter (-225 bp~+127 bp) was cloned by PCR. Subsequently, a recombinant adenoviral plasmid carrying KDR promoter for the HSV-tk gene was constructed with the AdEasy system. As a control, the plasmid pAdCMV-tk carrying CMV promoter for the HSV-tk gene was also constructed. 293 packaging cells were transfected by both newly-constructed plasmids and the infectious viruses AdKDR-tk and AdCMV-tk were generated. Then the KDR-producing cells and the KDR nonproducing cells (HepG2) were infected with AdKDR-tkand AdCMV-tk, followed by ganciclovir administration.

应用PCR克隆出人KDR基因启动子序列－225bp~+127bp，以AdEasy system为载体，构建携带受KDR启动子或CMV启动子调控tk基因表达的2种重组腺病毒质粒pAdKDR-tk和pAdCMV-tk，在293细胞中包装、扩增后，体外感染表达KDR的脐静脉血管内皮细胞系HUVEC和不表达KDR的肝癌细胞系HepG2，并给予不同浓度的丙氧鸟苷处理，5d后收集存活细胞并计数。

RESULTS: APC promoter 1A was methylated in NCI-H460 cells, and unmethylated in NCI-H446 and SPC-A1 cells. Hypermethylation was detected in all 5 CpG islands (687, 707, 714, 719, 726) of APC promoter 1A in NCI-H460 cells. The expression of APC in NCI-H460 cells was decreased by 26.04% of that in NCI-H446 cells and by 32.36% of that in SPCA1 cells. After treatment of 1, 5, 10, 15μmol/L 5-aza-dC, the expression of APC promoter 1A in NCI-H460 cells was enhanced by 4.59, 5.78, 9.58, 5.98 folds, respectively.

结果：SPC-A1和NCI-H446细胞APC甲基化阴性，NCI-H460细胞APC甲基化阳性；甲基化芯片检测Ncl-H460细胞在APC启动子1A 5个CpG位点均存在甲基化(687、707、714、719、726)，SPC-A1和NCI-H446甲基化阴性，荧光定量结果NCI-H460的APC转录较SPC-A1和NCI-H446有明显的下降，仅为二者平均的30.04%；经5-aza-dC脱甲基化作用后，NCI-H460细胞的APC表达增加了约5~10倍，其中10μmol/L浓度作用下，APC表达增加最多。

These constructs, together with the construct containing the GUS gene expression cassette under the control of the Hsp70 promoter, were utilized in the co-bombardment experiments to identify viral cistrons that could activate the transcription directed by the Hsp70 promoter. We found that the P1, K2NIa and CP cistrons could independently activate the Hsp70 promoter.

用这些瞬时表达载体与含有Hsp70基因启动子指导下的GUS基因的表达载体共轰击豌豆叶片，发现P1、K2NIa和CP能诱导Hsp70基因启动子的转录活性，P1最强，K2NIa次之，而CP最弱，同时还发现P1基因的功能区域在127碱基以后。

The split between the two groups can hardly be papered over.

这两个团体间的分歧难以掩饰。

This approach not only encourages a greater number of responses, but minimizes the likelihood of stale groupthink.

这种做法不仅鼓励了更多的反应，而且减少跟风的可能性。