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To investigate whether the expression of cdc2 and cyclin B1 in spermatogenic cells during spermatogenesis is actually a temperature dependent event, in situ hybridization, Western blotting and immunohistochemistry analysis were used to study the expression of cdc2 and cyclin B1 in normal and cryptorchid testis. Results showed that heat would differentially hurt male germ cells in different developmental stages during spermatogenesis, especially the pachytene primary spermatocytes. Most of spermatogonia in contralateral cryptorchid testis were not harmed fatally by heat as yet, indicating that spermatogonia could resist to beat to a certain extent. In this case spermatogonia could develop to pachytene/diplotene primary spermatocytes, but they could not acquire the ability to complete the transition from mitosis to meiosis, and then appeared to go through apoptosis. Therefore, we could not find the descendants of meiosis: secondary spermatocytes and round spermatids, elongated spermatids and spermatozoon. The abdominal temperature had no significant influence on the transcription of cdc2 and cyclin B1 in the spermatogonia and pachytene/diplotene primary spermatocytes. In normal rabbit testis, cyclin B1 increased in the pachytene/diplotene primary spermatocytes before meiosis and reached its peak in the spermatids.

为了解精子正常发生过程中cdc2和cyclin B1表达的低温依赖性，我们利用原位杂交和免疫组化等方法，研究了正常和隐睾精子发生过程中cdc2和cyclin B1的转录和翻译调控活动，结果表明：（1）热对各阶段的雄性生殖细胞都有损害，粗线期的初级精母细胞尤为敏感，实验性隐睾内的精原细胞尚未完全受到"致命"影响，说明精原细胞对热有一定的耐受性，但即使成为粗线期／双线期初级精母细胞，却未能获得由有丝分裂过渡到减数分裂的能力，呈现不同程度的凋亡，所以在整个切片中找不到源自减数分裂的产物----次级精母细胞、圆形精子细胞，更谈不上长形精子细胞和精子的形成；（2）腹腔高温未明显地影响隐睾精原细胞和粗线期/双线期初级精母细胞中cyclinB1和cdc2的转录，说明高温并不是通过影响cyclin B1和cdc2的转录活动而导致生精过程阻断的；（3）正常兔睾丸组织中，〓在精原细胞和粗线期／双线期精母细胞中均有表达：cyclin B1蛋白在减数分裂前期的粗线期／双线期初级精母细胞中的表达量增加，于变态末期的精子细胞中达高峰。

The results show that, when the volume of the secondary room is 1/3 of that of the primary room,the sound energy decay in the primary room is greatly in- fluenced by the secondary room at an aperture size of less than 10% of the wall,that the energy decay curve and the coupling con- stant in the primary room gradually change with the aperture size,and that the effect of the secondary room on the acoustics in the primary room is uniform.

发现在耦合空间体积与主厅体积比为1/3的条件下，当耦合窗面积与隔墙面积比小于10%时，耦合空间对主厅的声能衰减有较大影响；主厅的声能衰减曲线和耦合常数随开口的改变而逐渐发生变化：耦合空间对主厅各点音质的影响比较均匀。华南理工大学物理科学与技术学院；华南理工大学物理科学与技术学院广东广州 510640；广东广州 510640

In the primary structure of root, the primary xylem is diarch and the development of both the primary xylem and the primary phloem is exarch.

在根的初生结构中，其初生木质部为二原型，木质部及韧皮部的发育均为外始式。

In the sense of cutset, the relation between the rough set of a cutset and the cutset of a rough set, a fuzzy rough set of a fuzzy primary ideal in a semigroup is proved to be its fuzzy primary ideal. The sixed chapter is Rough Primary Ideals and Rough Fuzzy Primary Ideals in rings, the corresponding results of a semigroup are generalized in a ring. The last chapter is Rough-fuzzy Subsemirings in Semirings, First, the lower and upper rough-fuzzy semirings together with the left and the right ideal are defined.

利用截集意义下，截集的粗糙集与粗糙集截集的关系，证明了在完备同余关系下半群中的模糊准素理想的粗糙集是模糊准素理想；第六章是环中粗准素理想和粗模糊准素理想，将半群中的相(来源：A70BC论文网www.abclunwen.com)应结果推广到环中；第七章是半环中的粗模糊子半环，首先，给出上粗模糊子半环及上粗模糊理想等概念，并研究了它们的性质。

The characteristic of primary arc is analyzed, and the dynamic model of primary arc is established. The line differential equation including primary arc voltage is constructed, and the parameter estimation technique is used to estimate the voltage amplitude of primary arc, so as to determine the fault type and realize adaptive three-pole-autoreclosure.

分析了一次电弧的特点，建立了一次电弧的动态模型，利用一次电弧的特点建立了包含电弧电压的超高压输电线路相间短路的线路微分方程，通过参数估计的方法求得故障点的电弧电压，以判断故障类型，实现自适应三相重合闸。

Regarding the frequent occurrence of speed stalling of boiler axial flow primary fan in the operation of 600 mw unit in china,the cause of speed stalling was analyzed based on the actual measured data.the problem is mainly caused by primary air pipe resistance property variation,which caused the excessive opening of movable blades,the primary fan is working on the condition of higher wind pressure and lower flow.the approaches to reduce the speed stalling of primary fan and preventive measures are put forwa...

针对目前国内600 mw大容量机组锅炉轴流式一次风机运行中失速事故频繁发生的问题，在了解造成轴流式一次风机失速原理的基础上，根据实测数据对一次风机失速原因进行分析。主要原因为一次风管阻力特性的变化，使一次风机动叶开度过大，一次风机工作点处在高风压区低流量的工况点，是造成一次风机失速的主要原因，提出了运行中减少一次风机失速机率的处理方法及预防措施。

The results show that the undissolved silicon particles in the melt become the cores of primary silicon precipitated in solidification and there is a close relationship between the shape of primary silicon and undissolved silicon particles. The growth of silicon follows not only the twin plane re-entrant edge mechanism, but also layer mechanism as well. Meanwhile, the shape of primary silicon also relies on kinetic surroundings, such as the transmitting of solute. At higher overheating temperature, the shape of primary silicon becomes the star-shape and tree-shape.

结果表明：重熔过程中熔体中未溶解的硅相粒子，在凝固过程中可成为初生硅生长核心，并且未熔颗粒与初生硅形状之间存在明显对应关系；初生硅的生长机制不是惟一的，既可以以孪晶凹角机制生长，还可以以层状机制生长，初生硅最终形状还要取决于溶质传输等动力学环境；随着熔体过热温度的升高，凝固组织中初生硅形状由多边形向星形及树枝状转变。

The cells were cultured under the following serum microenvironment. The primary cells of autoserum group were cultured with autoserum, changing the medium with fetal bovine serum after passage. The primary cells of homogeneity foreign serum group were cultured with homogeneity foreign serum, changing the medium with fetal bovine serum after passage. The primary cells of fetal bovine serum group were cultured with fetal bovine serum, and cultured with fetal bovine serum after passage. The primary cells of Dulbecco's modified Eagle's medium group were cultured with serum-free DMEM, changing the medium with fetal bovine serum after passage.

分别以下列血清微环境培养：自身血清组：分离细胞后原代用含大鼠自身血清的培养基培养，传代后换用含胎牛血清的培养基培养；同种异体血清组：分离细胞后原代用含同种异体血清的培养基培养，以后用胎牛血清培养基培养；胎牛血清组：分离后用含胎牛血清培养基培养，以后一直用含胎牛血清培养基培养；DMEM组：分离细胞后原代用不含血清的DMEM培养基培养，传代后换用含胎牛血清的培养基培养。

TiC has a refinement to both primary and eutectic borides, some of which can act as the substrate for primary boride making refinement, the remainder inserting into the primary boride imped it growing in matrix; TiC inserting into the primary boride impedes the eutectic boride growing on the primary boride and decreases the formation of large block eutectic boride.

TiC对初生硼化物和共晶硼化物均有细化作用：一部分作为初生硼化物异质形核核心使其细化，另一部分在基体中嵌入初生硼化物阻碍其生长；嵌入初生硼化物的TiC改变了共晶硼化物依附初生硼化物的特点，使共晶硼化物分布细小均匀。

To dec.2002 were analysed by means of wounding tract and fracture.results during 24 cases of gunshot wound, wounds of 20 cases healed without event after primary debridment and sewing wounds,3 cases healed and l case infected in 4 cases of primary debridment without suture,and 4 cases of primary internal fixed fracture had no osteomyelitis.the injury of soft tissue and contamination of wound track were serious in explosion wound than in gunshot wound,10 cases healed without event and 4 cased infected in l4 cases of primary closed explosion wounds,and 2 cases of primary inernal fixed fracture had no osteomyelitis.conclusion it is suggested that the management of firearm wound in cold and dry region may be delayed moderately in time of debridement.the firearm wound may be treated by internal fixation of fracture and primary closing of tract after early debridment when the soft tissue injury was light in peacetime, which decreased the infection,disability and promoted rehabilitation of patients.

结果 24例枪伤中，20例一期缝合伤口，均一期愈合，另外4例只清创未一期缝合的伤口，3例愈合，1例感染；4例骨折内固定均愈合良好，无骨髓炎发生。16例炸伤病人局部软组织损伤及污染比枪弹伤重，14例一期缝合伤口中，10例一期愈合，4例感染，骨折内固定2例，均愈合良好，无骨髓炎发生。结论寒冷干燥地区火器伤早期伤道清创时限可适当延长，在平时火器伤的救治中，对软组织条件较好的创面，早期清创后可一期闭合伤口和骨折内固定，这样可减少感染和伤残，促进康复。寒冷干燥地区；火器伤；早期救治

Do you know, i need you to come back

你知道吗，我需要你回来

Yang yinshu、Wang xiangsheng、Li decang,The first discovery of haemaphysalis conicinna.

1〕 杨银书，王祥生，李德昌。安徽省首次发现嗜群血蜱。