查询词典 Picard group

Part II The experiment study on anti-tumour effect of dendritic cells derived from peripheral blood monocyte of patients with oral cancer after pulsed by tumour antigen in different ways. Collected 50ml peripheral blood from each patients and volunteers to induced DC in vitro. We parted the test group and control group into three subgroups respectively, untreated group A, freezed and thawn group B, RNA transfected group C. To group A, after induced DCs for five days , we added 200U/mlTNF-α in each hole and cultured on. To group B, we added protein antigen (DC/ tumor cells = 1 : 1 ,each hole) for 4h at 5d, and then added 200U/mlTNF-α in each hole and cultured on. To group C, transfected immature DC with tumor cell RNA(DC:RNA=1×105/ml: 5ug)at 5d, operated completely with the specification of invitrogen DMRIE-C reagents. After transfection we replaced transfection agent with complete medium, and then added 200U/mlTNF-α in each hole and cultured on.

第二部分口腔癌患者外周血来源的树突状细胞体外负载抗原后抑瘤作用的实验研究无菌采集20例口腔癌患者和20例健康志愿者外周血50ml，分离单个核细胞体外诱导培养DC，并将实验组和对照组细胞各分为三组，A组为未处理组，B组为冻融组，C组为RNA转染组。A组DC在培养5d后，每孔加入TNF-α200U/ml；B组DC培养第5d后，每孔中加入癌细胞蛋白抗原，使DC/肿瘤细胞=1：1，继续培养4h后，每孔加入TNF-α200U/ml；C组将培养至第5d的未成熟DC用肿瘤细胞RNA进行转染(DC：RNA=1×105/ml： 5ug，按照invitrogen DMRIE-C试剂说明书进行操作)，转染结束后用完全培基取代转染剂，每孔加入TNF-α200U/ml。

There were five groups in the examination of cellular levelK562 group,K562/A02 group,K562+ADM group,K562/A02+ADM group and K562/A02+TTD+ADM group）.The non-cytotoxicity doses to cell lines K562 and K562/A02 of TTD were got by MTT assay.Using flow cytometry (FCM assay to examine the intracellular ADM concentration.There were three groups in the examination of genic,zymologic and protein levelsK562 group,K562/A02 group and K562/A02+TTD group）.The mRNA expression of MDR was measured by fluorescent quantitative reverse transcriptase polymerase chain reaction(RT-PCR.The expression levels of glutathione-S-transferase and topoisomerase Ⅱ were determined by immunohistochemical technique.

细胞水平检测实验分5组（K562组、K562/A02组、K562+ADM组、K562/A02+ADM组和K562/A02+TTD+ADM组），采用MTT法检测TTD对K562和K562/A02细胞的非细胞毒性剂量，流式细胞术检测细胞内阿霉素的浓度，基因、酶学、蛋白水平检测实验分3组（K562组、K562/A02组和K562/A02+TTD组），采用RT-PCR法检测mdr1 mRNA的表达，免疫细胞化学方法检测谷胱甘肽S转移酶π和拓扑异构酶Ⅱ的表达水平，Western-blotting法检测P-糖蛋白和bcl-2表达。

Result:1:in the aspect of controlling of the swelling development, treatment group is distinctly superior to control group.2:in the aspect of deswelling rate,treatment group is superior to control group.3:in the aspect of controlling pain degree,shorten the applying time of the anodyne, treatment group is distinctly superior to control group.4:in the aspect of controlling the large-area tension bullae incidence,treatment group is superior to control group.

在消肿速度上，治疗组优于对照组。3。在控制疼痛程度，缩短止痛药应用时间方面，治疗组明显优于对照组。4。在控制大面积张力性水泡发生率上治疗组优于对照组。

Afterwards through the entire audience representative's secret ballot, the selection has produced the human interspace free federation management structure member is the human interspace management group, the group core member name list distinction is as follows: Group leader: Entire unification, authority: Coordinated entire humanity all important business; Military vice-group leader: Yellow Jinlong, authority: Is responsible for entire human all foreign defense business; Lives vice-group leader: Jumbles together, authority: Is responsible to coordinate the humanity to live aspect all business; Produces vice-group leader: White Peter, is responsible to coordinate the human all production activity; Eliminates this is cultural vice-group leader: Black must win, is responsible for the human all culture energetic domain business; Each group is 1000, the prime task assists fellow main members, develops all to serve together the overall humanity's great enterprise!

随后通过全场代表的无记名投票，选取产生了人类星际自由联邦的管理机构成员即人类星际管理小组，小组核心成员名单分别如下：组长：全统一，职权：协调整个人类一切重要事务；军事副组长：黄金龙，职权：负责整个人类一切对外防卫事务；生活副组长：混为一谈，职权：负责协调人类生活方面一切事务；生产副组长：白彼得，负责协调人类一切生产活动；除此就是文化副组长：黑必胜，负责人类一切文化精神领域的事务；各部组员为1000名，主要工作是协助各位主要成员，共同开展一切服务于整体人类的伟大事业！

48 SD rats were randomly di2 vided into eight groups : rest group , exercise group , low pollution exercise group , low pollution rest group , middle pollution exercise group , middle pollution rest group , high pollution exercise group and high pollution rest group .

48只SD雄性大鼠随机分为8组：空白对照组、单纯运动组、低污染运动组、低污染安静组、中污染运动组、中污染安静组、高污染运动组和高污染安静组。

Company main products are: K-type separator, O-Sepa Separator, Combined Separator, coal grinding Dynamic Separator, Separator dedicated slag, fly ash dedicated separator, the Kowloon-Tian Dryer, pulse bag filter offline, brick-concrete structure bag filter, the new line pulse jet bag filter, as well as other types of large bags and electrostatic dust removal equipment, such as 6 big series more than 100 varieties, has to assume "15" provincial IT industry research and a national innovation fund projects the province of major scientific and technological achievements into a project by the national key new products, a provincial high-tech products 8, the Provincial Torch Plan 2, with 46 patents, including one for the International Invention, these sophisticated, applicable technology and equipment have been for the Conch Group, the Landscape Group, Huaxin Group, Yifeng Yancheng, Tianrui Group, Yatai Group, Jinding Group, an Ha Group Jiangsu Yangtze River, Lei Group, Vietnam Yaguang, Bangladesh Shillong, etc.

公司主产品有：K型选粉机、O-Sepa选粉机、组合式选粉机、煤磨动态选粉机、矿渣专用选粉机、粉煤灰专用选粉机、九田立式烘干机、离线脉冲袋式除尘器、砖混结构袋式除尘器、新型行喷脉冲袋除尘器以及其它各类大型布袋和静电除尘等设备6大系列100多个品种，先后承担&十五&省科技工业攻关和国家创新基金项目省重大科技成果转化项目各1个，获国家重点新产品1个，省高新技术产品8个，省火炬计划2个，拥有46项专利，其中1项为国际发明，这些成熟、适用的技术装备已先后为海螺集团、山水集团、华新集团、宜丰盐城、天瑞集团、亚泰集团、金顶集团、安厦集团、江苏长江、磊达集团、越南亚光、孟加拉西隆等2000余家水泥、石膏、钢铁、电力企业服务。

Results By incubating cardiomyocytes with bFGF, ERKs was activated and the cellular viability 〔(70.0±4.6)%in bFGF 10 ng group vs.(53.0±4.5)%in H/R group, P＜0.01） and the ATP content 〔(23.1±2.3) nmol/106 cells in bFGF 10 ng group vs.(12.3±2.1) nmol/106 cells in H/R group, P＜0.01〕 were increased, also the LDH leakage 〔(257.3±51.0) U/L in bFGF 10 ng group vs.(372.5±69.2) U/L in H/R group, P＜0.05〕, was decreased, then attenuation of H/R injury to cardiomyocytes was observed. PD098059, an inhibitor of upstream kinase of ERKs completely abolished the protective effects 〔viability (57.0±5.8)%,ATP content (15.1±2.6) nmol/106 cells,LDH activity in medium (325.5±59.0)U/L in PD098059 group vs. bFGF 10 ng group, P＜0.01, respectively〕.

结果 bFGF激活ERKs，并呈剂量依赖性减轻H/R所致心肌细胞损伤，表现为细胞存活率〔bFGF 10 ng组(70.0±4.6)%，H/R组(53.0±4.5)%，P＜0.01〕和细胞内ATP含量〔bFGF 10 ng组(23.1±2.3)nmol/106细胞，H/R组(12.3±2.1)nmol/106细胞，P＜0.01〕升高，细胞浆酶LDH漏出〔bFGF 10 ng组（257.3±51.0）U/L，H/R组（372.5±69.2）U/L，P＜0.05〕减少；ERKs上游激酶抑制剂PD098059完全消除上述保护作用〔PD098059组细胞存活率（57.0±5.8）%，ATP含量（15.1±2.6）nmol/106细胞，培养液中LDH活性（325.5±59.0）U/L，与bFGF 10 ng组比较均为P＜0.01〕。

Thirty-six rats ,weighing(250±10)g, were randomly divided into normal group, experimental group and control group.Orthodontical devices were put between the upper incisor teeth and dens molaris in both experimental group and control group.The corrective force was adjusted to 60g.Salvia miltiorrhiza combination was implanted to each rat in the experimental group every day.The animals of both experimental group and control group were killed at 7,14 and 21 days.The upper dens malarias and periodontal tissue slices were observed under the light microscope and transmission electro microscope,and the X-ray dental films were taken and measured with digitization dental scanning apparatus and its software.

以36只体重(250±10)g的雄性大鼠为样本，随机分为正常组、实验组和对照组，设定不同浓度丹参复合膜，以同体对照方式在正常组进行体内植入实验，在2周内观察不同浓度丹参的牙周组织诱导作用，寻找最佳应用浓度；在实验组和对照组的上切牙与磨牙之间隙安装正畸装置，建立大鼠磨牙移动实验模型，矫治力的大小为60g；实验组每日每只体内植入丹参复合生物膜，两组动物于正畸加力7、14、21d，分三批处死；处死后立即切取双侧上颌磨牙及牙周组织制取标本，制片透射电镜和光镜观察；拍摄X线牙片，用数字化牙科扫描仪及软件对其进行测量分析。

Learning-theory has an apparent improving effect on students" learning the basic skill of table-tennis. In the comparison between tested group and compared group, an obvious difference appears; it also has a positive effect on students" learning interest, for it can make students keep more interest in learning; using learning-theory is able to improve students learning method of table-tennis. Based on the similar initial learning level of tested group and compared group, and through a one-term teaching experiment, apparent difference of learning method between tested group and compared group arrears, and the learning method of tested group is better than that of compared group.

学习理论对学生学习乒乓球的基本技术有明显的促进作用，在实验组与对照组的比较中表现出显著性差异；对学生的乒乓球学习兴趣也存在积极的影响，可以使学生保持较高的学习兴趣；学习理论应用到乒乓球教学中还可以提高和改善学生的乒乓球学习方法，在实验组与对照组学习方法基线水平无显著性差异的基础上，通过一学期的教学实验发现，实验组与对照组在学习方法上出现显著性差异，实验组的学习方法好于对照组。

Results: The age of onset, the proportion of smoke, the proportion of cough, expectoration, polypnea, cyanosis, rhonchi and moist rale in two lungs were higher in OS patients than OSAS group, the level of body mass index, neck circumference, the proportion of polypnea, nighttime snore, nighttime apnea, daytime hypersomnolence were higher in OS patients than COPD group, the proportion of heart、pulmonary、kidney、 encephalopathy were higher in OS patients than OSAS group and COPD group, FVC, FEV1, FEV1/FVC, MVV, PaO2, SaO2 were lower in OS patients than OSAS group, FRC, PaCO2 were higher in OS patients than OSAS group, AHI was inversely related to the FEV1, FVC, MVV in Group OS, LSaO2 in Group OS was inversely related to the AHI.

结果：OS组的发病年龄、吸烟者比例、咳嗽咳痰、气促、紫绀、肺罗音比例均高于OSAS组，BMI、NC、夜间打鼾、夜间呼吸暂停、白天嗜睡的比例均高于COPD组；OS组合并心、肺、肾、脑等疾病的比例均高于OSAS组及COPD组；OS组的FVC、FEV1、FEV1/FVC、MVV、PaO2、SaO2的值均低于OSAS组，FRC、PaCO2均高于OSAS组；OS组AHI与FEV1、FVC、MVV呈负相关，LSaO2与AHI呈负相关，LSaO2与FEV1、FVC、MVV呈正相关；OS组的最长呼吸暂停时间、S1+2、NREM均高于OSAS组，而LSaO2、REM均低于OSAS组，AHI、最长呼吸暂停时间、S1+2均高于COPD组，LSaO2、S3+4均低于COPD组。

Objective To study the effect of chitosan in the prevention of fibrous scar formation in the epidural space after laminectomy.

目的 观察选择性脊神经后根切除术椎板切除后硬膜外几种防瘢痕粘连物质的作用并探讨其机制。

On his way to the lift, he looked back to us or was it me his eyes pointing?

当他要进电梯时，他回头看我们（或者说是我，他的眼光？