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The N gene ORF was then subcloned into pET-30a vector and the recombinant plasmid was transformed into E.coli BL21 (DE3) and induced with IPTG. The protein expression was determined by SDS-PAGE. The expressed protein had a molecular weight of 54.4 Ku that existed as inclusion body. Thin-layer scanning showed that the expression product accounted for 30.5% of the total bacterial proteins. The recombinant protein possessed native biological activity and could react with anti-PEDV hyperimmune serum in Western blot.

以阳性质粒为模板,用分别含有BamH Ⅰ和Xho Ⅰ酶切位点的上、下游引物扩增得到ORF,其PCR产物经BamH Ⅰ和Xho Ⅰ双酶切后定向克隆到pET-30a载体,构建的重组质粒命名为pET-30a-PN;将pET-30a-PN转化到大肠杆菌BL21 (DF3)中,在IPTG诱导下进行表达;SDS-PAGE结果表明表达出与预期大小相符的约54.4 Ku的重组蛋白,重组蛋白以包涵体形式存在;薄层扫描结果表明表达产物占菌体总蛋白的30.5%;Western blot分析表明表达的重组蛋白能与抗PEDV高免血清反应,说明该重组蛋白具有免疫学活性。

The analysis of partial correlation and path on Pn and factors shows that the diurnal variation of Pn has significant correlation with photosynthetically active radiation in Summer.

偏相关分析和通径分析表明:夏季光合有效辐射(X1)与净光合速率的日变化有着显著的相关关系

Treating with different concentration of PEG 6000 in 1/2 Hoagland nutrient solution for 3 days, the physiological indexes , such as, Pn, chlorophyll fluorescence parameters, cell membrane permeability, MDA and relative water contents of Plantago asiatica L. and Viola philippcia Cay, were studied. The Pn of P. asiatica and V.

在不同浓度的PEG6000的1/2Hoagland营养液水分胁迫条件下,对处理3d的车前和紫花地丁的净光合速率、叶绿素荧光参数、细胞电解质外渗率、相对含水量和丙二醛含量等指标进行了测定和分析。

Chapter 2 discusses the function of optimum array based on the minimum meansquared error principle in CDMA system, the method of produce the reference signal, and proved whether the reference signal is the correspond PN code sequence or respread signal, the result of the array's weight converge can converge the same optimum weight under the condition that the PN sequence in a bit of data is long enough. In this chapter, we finally get the beam pattern and the correlate bit error rate after converge when several CDMA signal arrives in different direction.

本文的主要贡献及创新之处是:首次从理论上探讨了基于最小均方误差准则的最优天线阵在CDMA系统中的多址干扰抑制性能及其参考信号的产生方法,证明了采用有用信号相应的扩频码作为参考信号与采用将解扩信息与扩频码进行重扩频后的信号作为参考信号来调节自适应天线的权值均可使权值收敛于同一最优解。

Under light water stress, the main reason is the stomatic limitation; under heavy water stress that is nonstomatic limitation.

轻度水分胁迫(SRWC在60%~40%)下,气孔限制是Pn下降的主要原因;严重水分胁迫(SRWC在40%~20%)下,Pn的下降主要是非气孔限制的结果。

The following results were found in the experiments: in pot experiments the diurnal variation of net photosynthetic rateof Lianmeng and Duizhao presented a single-peak curve, and the maximum value occurred at about 12:30, the diurnal variation of net photosynthetic rate of Changlongjie was unregular, the photosynthetic capacity of three treatments from high to low was Lianmeng > Duizhao> Changlongjie, heavy metals stress had an inhibitory effect on photosynthesis of plant; There was a significant positive correlation between stomatal conductance and transpiration rate during the diurnal variation(Lianmeng 0.981**, Changlongjie 0.829*, Duizhao 0.87*).

结果表明:在白玉兰盆栽试验中,联盟和对照的Pn日变化呈&单峰型&曲线,峰值都出现在12:30左右,长龙界的Pn日变化无规律,3个处理的光合能力为联盟>对照>长龙界,重金属胁迫对植物的光合有抑制作用。

At forces that depend on the attachment geometry of the DNA (65 pN or 110 pN), it elongates ≈1.7-fold and its elastic properties change dramatically.

这些结果可能为理解DNA-蛋白质的相互作用以及DNA的热动力学——它们对于包括细胞分裂和转录在内的一些细胞过程至关重要——提供一个基础。

Moreover, the nitrated tau showed a significantly decreased binding activity to taxol-stabilized microtubules in vitro.

为阐明tau蛋白的硝基化在AD的病理过程中如何发挥作用,我们在体外用PN处理纯化的人重组tau蛋白,结果显示PN浓度依赖性地诱导tau蛋白发生硝基化和寡聚化,而且硝基化tau蛋白与微管的结合活性显著下降。

The structure consists of several modules, among them the reference PN model syncretizes the affection of resources restriction and a monitor PN model watch the happening of accidents. So the manager can know the situation of project clearly, and repair the planning and scheduling strategy to fit for the new condition.

方法中参考Petri 网模型考虑到生产资源约束的影响,监控Petri网模型实时监视突发事件的发生,从而项目管理者可以及时地了解项目的进展,并根据实际生产情况对现有的项目计划调度任务的进行动态调整。

According to the cross correlation between the PN signal and the auxiliary signals with the different phases, an exact and valid estimation of improving direction and magnitude for the local PN generator is given, by which half the mean acquisition can be reduced.

它能根据输入信号与一个特殊序列不同相位下的互相关值,相当准确、有效地估计出本地伪随机序列的改进方向和大小,成倍地缩短了捕获的时间。

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The split between the two groups can hardly be papered over.

这两个团体间的分歧难以掩饰。

This approach not only encourages a greater number of responses, but minimizes the likelihood of stale groupthink.

这种做法不仅鼓励了更多的反应,而且减少跟风的可能性。

The new PS20 solar power tower collected sunlight through mirrors known as "heliostats" to produce steam that is converted into electricity by a turbine in Sanlucar la Mayor, Spain, Wednesday.

聚光:照片上是建在西班牙桑路卡拉马尤城的一座新型PS20塔式太阳能电站。被称为&日光反射装置&的镜子将太阳光反射到主塔,然后用聚集的热量产生蒸汽进而通过涡轮机转化为电力