查询词典 P.T.O.

Solving this pr o blem is my t o p priority.

解决这个问题是我最优先考虑的事。

Yet there is only so much Rodriguez can do. For all his sparkling numbers, the only one that matters for the Yankees is .500. That is their winning percentage after stranding 16 runners on Saturday and losing to the San Francisco Giants, 6-5, on a bloop single by Nate Schierholtz in the 13th inning.7 K; \. O; t! S; W/ V v

{1 v7 l* p， q+ k A-rod可以帮洋基做很多事，在A-rod精彩表现的数字上，在星期六对上巨人队，留下了十六个残垒，而在第十三局时，因为Schierholtz穿出内野的一垒安打而输掉了比赛后，唯一让洋基最在意的是那五成的胜率。

P a t, x9 x$ _5 _ Pyrrho, the doyenne of the Hellenistic philsophers known as the Sceptics, promoted that the best way to live life was without judgement, to neither invest in feeling or belief.

3 ？： S% X v9 l7 g*}； O pyrrho，作为著名的古希腊极端怀疑主义哲学家，她提出最好的生活方式就是不去做任何判断，也不把精力用于感受与信任。

O/ q, b$ t! H4 K- P/ t/ b You say: I feel half-cooked.

你说：我感觉自己半生半熟。

Perhaps you can work out the details for me, Prince Toshihito? And Miss Sackville-West, I'll need your horticultural assistance, to help me plan a gradient of colors and textures from the lower to the upper end. Jessamine clings to the walls…. T+ I$ O2 3 c* I7 V5 s

对了，智仁亲王(日本名园桂离宫的建造者)，你可以帮忙弄些细部；萨维斯特小姐(英国名园希辛赫斯特的植栽设计者)，你可帮忙配植栽，帮忙计画色彩与质感；也许，墙面上爬些茉莉……' z6 Z， o* _， R L7 p* D9 b4 x

12 D osage sho uld be adapted t o p atients i ndividually, on t he basis of pe riodic tes ts of

在周期性检验尿糖和血糖的基础上，调整剂量，使之适用于不同的患者。

Those like shit are:(from 12 o'clock clockwise direction) dried chili, onions, shallots, garlic...and very imporant is belacan powder, and a bottle of sambal oelek so that you don't need to make so much on your own :P

那几陀被搅成稀巴烂的是：（12点起顺时钟）辣椒干，大洋葱，红色小洋葱仔，蒜米。。。还有很重要的belacan粉，和sambal oelek一罐（这样就不用自己弄这么多咯：P）。。。

Of P I pat hways i n pl ant development and cel l respo nse t o en vironmental fact ors, t he f unctional

表达谱及其生理功能的相关分子机制和与其它信号途径之间的相互作用。

By use of site mutation strategy and PCR technology, we obtained the gene P12X3C that includes full length PI, 2A, 3C and a part of 2B and 3B and the gene P12X3C3D that includes full length PI, 2A, 3C, 3D and a part of 2B and 3B. After being digested by restriction enzyme respectively, the gene P12X3C and the gene P12X3C3D were cloned into the pcDNA3.1 and pTARGET expression vector that were digested by the same enzyme. Recombinant plasmids were checked by restriction enzyme analysis and nucleic acid sequencing. Further more, recombinant plasmids were transfected into BHK-21 cells by using lipoid. The proteins of foot-and-mouth disease virus, which were expressed in BHK-21 cells, were confirmed by sandwich-ELlSA and fluoroscopy, and the capsid of FMDV was tested by electron microscope. In order to evaluate enhanced immune response of guinea pigs against FMDV, DNA vaccines which were designed to produce viral capsids lacking infectious viral nucleic acid and contained the gene P12X3C and the gene P 12X3C3D were injected respectively with FMDV 3D protein which was expressed in Pichia Pastoris Secreted expression System and purified or with pcDNA3.1/lFN which includes the gene IFN-a of cattle. Subsequently, Recombinant plasmids were injected to catties with or without pcDNA3.1/IFN. Anti-FMDV antibodies were detected by ELISA, and the T lymphocyte proliferation response was tested by MTT assay, neutralization antibodies liters were analyzed by micro-neutralization assay.

为研制带有O型口蹄疫病毒(Foot-and-Mouth Disease Virus，FMDV)China99株结构蛋白基因及多个非结构蛋白基因的DNA疫曲，本研究通过定点突变方法和PCR扩增方法，获得包含有FMDV China99株结构蛋白P1、非结构蛋白2A、3C以及部分2B、3B编码基因的片段P12X3C和包含有FMDV China99株结构蛋白P1、非结构蛋白2A、3C、3D以及部分2B、3B编码基因的片段P12X3C3D，将获得的基因片段直接／酶切后与同样处理的真核表达质粒连接，分别得到重组质粒pcDNA3.1／P12X3C和pcDNA3.1／P12X3C3D、pTARGET／P12X3C3D；对重组质粒进行序列测定、分析，并将重组质粒分别转染BHK-21细胞，通过双抗体夹心ELISA方法和间接免疫荧光标记方法检测细胞中FMDV抗原的表达，用电子显微镜观察病毒空衣壳的组装；为评价重组质粒作为DNA疫苗对实验动物及本动物的免疫效果，将重组质粒经肌肉注射方法接种豚鼠，并与酵母表达的纯化FMDV China99株3D蛋白及带有牛α干扰素的真核表达质粒pcDNA3.1／IFN分别／同时免疫，第二次免疫后第三周豚鼠攻以100ID_(50)或1000ID_(50)的O型FMDV China99株：随后将质粒pcDNA3.1／P12X3C、pcDNA3.1／P12X3C3D与带有牛α干扰素的真核表达质粒pcDNA3.1／IFN同时免疫牛，三周后经牛舌皮攻以10~4ID_(50)的O型FMDV China99株。

P- \% v/ E; o; T* O# M Now the next step will be to do a final cleanup, adding a flowered sundress(flowers, butterfly - get the picture?).

现在，下一步是做一些最后的清洁，加一个花花夏装（花，蝴蝶——看到图片了？

Do you know, i need you to come back

你知道吗，我需要你回来

Yang yinshu、Wang xiangsheng、Li decang,The first discovery of haemaphysalis conicinna.

1〕 杨银书，王祥生，李德昌。安徽省首次发现嗜群血蜱。