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The results showed that the rates of mature oocyte were 75.2%, 73.1%, 69.8%, 63.5%and oocyte at telophase of MI were 16.3%, 15.9%, 16.9%, 27.0%, respectively. The rates of maturation of oocytes cultured in TCM199 with serum, EGF and TGFαwere significantly higher than that of with BSA team (P<0.05) and the number of oocyte stayed at telophase of MI in TCM199 with serum, EGF and TGFαteam were significantly less than that with BSA team(P<0.05); Significant higher rates (66.6%, 66.6%, 73.6%) of normalα-tubulin distribution in oocytes cultured in TCM199+ serum, EGF and TGFαwere compared to that of TCM199+BSA(43.3%)(P<0.05). The rates of oocyte with cortical granules in cortex were 58.8%, 33.9%, 54.7%and 47.9%respectively, there was significant difference between oocytes cultured with serum, EGF and BSA(P<0.05). In conclusion, TGFαand EGF can promote the oocyte nuclear transition from telophaseⅠto metaphase of meiosisⅡ, and improved the expression and distribution ofα-tubulin during the ovine oocytes maturation; EGF and serum could promote oocyte cytoplasm maturation. The results suggested that EGF and TGFαmight substitute some substance in serum to improve the quality of oocyte nucleus maturation in vitro, but EGF might be more functional than TGFαto promote the maturation of ovine oocyte ooplasm.

三、EGF和TGFα对卵母细胞成熟的影响未成熟卵母细胞分别在TCM199基础培养液+血清、TCM199基础培养液+BSA+EGF、TCM199基础培养液+BSA+TGFα和TCM199基础培养液+BSA四组成熟培养系统中成熟培养,22小时后上述各组卵母细胞的核成熟率分别为75.2%、73.1%、69.8%、63.5%,处于第一次减数分裂末期的比率分别为16.3%、15.9%、16.9%、27.0%,在添加血清、EGF、TGFα各组中核成熟率显著高于其它各组(P<0.05),处于末期的比例明显低于其它各组(P<0.05):添加血清、EGF、TGFα各组在a-Tubulin蛋白的分布与表达上(66.6%、66.6%、73.6%)明显高于BSA组的正常率43.3%(P<0.05);各组CG发生迁移较好的卵母细胞的比率分别为58.8%、33.9%、54.7%、47.9%,血清组和添加EGF组CG迁移至皮质区的比例明显高于仅添加BSA处理组(P<0.05)。

The type 2 diabetes-mellitus (T2DM) subjects had higher HOMR-IR, FINS, FPG, TC, TG, LDL and lower ISI than NC subjects. The 2DM subjects with genotype Thr54 (Thr54 homozygotes and heterozygotes) had higher FPG、FINS、HOMR-IR、TG、LDL(P.01) and lower ISI (P.01) than those with genotype Thr (Ala54 homozygotes). Conclusions: The polymorphy of FABP2 has been identified in the Han nationality in Bengbu.

与NC组比较,T2DM组的胰岛素抵抗指数、胰岛素均明显增高(P.01),胰岛素敏感指数显著降低(P.01);甘油三酯、总胆固醇、低密度脂蛋白均明显增高(P.01),高密度脂蛋白明显降低(P.01)。T2DM组中,FABP2Ala54Ala与Ala54Thr及Thr54Thr基因型患者比较,后两者的空腹胰岛素、HOMA-IR指数、空腹血糖、甘油三酯水平、低密度脂蛋白明显增高(P.01),而胰岛素敏感指数显著降低(P.01)。

Results The mortality rate of mice in 80 mg/kg, day cyclophosphamide group was 16.7%, and T level [ at 30th day :( 1.38 ± 0.31 );45th day:( 1.15 ± 0.26 ) ] and T/LH ratio [ at 30th day:(0.163 ± 0.014); 45th day:(0.127 ± 0.023 ) ] were significantly decreased (all P<0.05) at 30th day after induction;The concentration of MDA [at 15th day:(2.70 ± 0.41);30th day:(2.710.36);45th day:(2.67 ±0.43) ] was maintained at a high level (all P<0.05) during the 45 days ; Number of Leydig's cells [ at 15th day:(9.65 ± 0.75 ); 30th day:( 14.05 ± 0.67 ); 45th day:(8.49 ± 072)] and layers of spermatogenetic epithelia [ at 15th day:(4.75 ± 0.82);30th day:(3.60 ± 0.49);45th day:(3.74 ± 0.43 ) ] were significantly decreased ( all P < 0.01 ) and stabilized in a low level. The induced model was stable and the mortality rate was acceptable. In the 60 mg/kg, day cyelophosphamide group, the T level and T/LH ratio had no significant change (P > 0.05 ), and the concentration of MDA ,number of Leydig' s cell and layers of spermatogenetic epithelia recovered at 30th day after induction. The induced model was unstable.

结果 剂量每日为80 mg/kg体重小鼠成模后死亡率为16.7%,血清T[30 d:(1.38±0.31);45 d:(1.15±0.26)]及T/LH比值[30 d:(0.163±0.014);45 d:(0.127±0.023)]于诱导后第30天出现显著下降(P均<0.05),而诱导后睾丸组织内MDA含量[15 d:(2.70±0.41);30 d(2.71±0.36);45 d:(2.67±0.43)]维持高水平(P均<0.05),生精上皮层次[15 d:(4.75±0.82);30 d:(3.60±0.49);45 d:(3.74±0.43)]和间质细胞[15 d:(9.65±0.75);30 d:(14.05±0.67);45 d:(8.49±0.72)]均显著减少(P均<0.01)并稳定于低水平,模型稳定,死亡率适当;每日60mg/kg体重组小鼠血清T及T/LH比值于不同时段并未出现明显变化(P>0.05),且睾丸组织内MDA含量、生精上皮层次和间质细胞计数在30 d后有所恢复,模型不稳定;每日100 rag/ks体重组死亡率为30.0%,死亡率过高。

Based on an intention-to-treat analysis, patients treated with pioglitazone had a significant increase in limb fat compared with those receiving placebo (0.38 vs 0.05 kg; P =.051). No difference was found, however, in the patients treated with stavudine. After excluding these patients, the increased limb fat was even greater for the pioglitazone-treated patients (0.45 vs 0.04 kg; P =.013). Other improvements included increased thigh circumference (1.4 vs 0.2 cm; P =.017) and tricipital skin-fold thickness (0.9 mm vs 0.4 mm; P =.047). No difference was found in subcutaneous abdominal fat or visceral fat.

根据意向分析,接受pioglitazone治疗相较于接受安慰剂的病患,肢体脂肪显著地增加(0.38相较于0.05公斤;P=。051);除了使用stavudine治疗的病患外,并没有发现差异;在排除这些病患后,接受pioglitazone治疗的病患,肢体脂肪显著地增加(0.45公斤比0.04公斤;P=。013);其它变化包括大腿围增加(1.4相较于0.2公分;P=。017),以及三头肌皮肤厚度(0.9公厘相对于0.4公厘;P=。047),至于皮下脂肪或是脏器脂肪并没有差异。

Based on an intention-to-treat analysis, patients treated with pioglitazone had a significant increase in limb fat compared with those receiving placebo (0.38 vs 0.05 kg; P =.051). No difference was found, however, in the patients treated with stavudine. After excluding these patients, the increased limb fat was even greater for the pioglitazone-treated patients (0.45 vs 0.04 kg; P =.013). Other improvements included increased thigh circumference (1.4 vs 0.2 cm; P =.017) and tricipital skin-fold thickness (0.9 mm vs 0.4 mm; P =.047). No difference was found in subcutaneous abdominal fat or visceral fat.

根据意向分析,接受pioglitazone治疗相较於接受安慰剂的病患,肢体脂肪显著地增加(0.38相较於0.05公斤;P=。051);除了使用stavudine治疗的病患外,并没有发现差异;在排除这些病患后,接受pioglitazone治疗的病患,肢体脂肪显著地增加(0.45公斤比0.04公斤;P=。013);其他变化包括大腿围增加(1.4相较於0.2公分;P=。017),以及三头肌皮肤厚度(0.9公厘相对於0.4公厘;P=。047),至於皮下脂肪或是脏器脂肪并没有差异。

The results indicated that the growth of 3-year-old Prunus salicina was significantly higher than that of the other species, such as 139.1% and 153.7% higher than Toona sinensis and Juglans regia in height growth, respectively. The maximum percentage of DBH more than 1.0 cm was 91.9% for P. salicina. Next were 49.5% and 48.8% for Ulmus pumila and Pteroceltis tatarinowii, respectively. The contents of total N, total P and total K ranged from 1.36% to 2.52% in the leaves of testing species. The highest content of total N and total P was found in the leaves of Catalpa bungei, while the highest content of total K was observed in P. saltcina leaves. The results indicated that P. salicina, P. tatarinowii, U. pumila had well adaptability on the Karst area both in the growth status and the improvement to the soil with the fallen leaf.

结果表明:(1)3年生冰脆李的生长量明显优于其他树种,其高生长比香椿、核桃分别大139.1%和153.7%;植株胸径大于等于1.0 cm株数的百分比以冰脆李最高达到91.9%,其次是榆树和青檀,分别是49.5%和48.8%;(2)8个树种落叶的全N、全P和全K的总含量变动为1.36%~2.52%,其中全N、全P含量以滇楸最高,全K含量以冰脆李最大;(3)从各树种落叶前后各元素含量及其转移情况看,冰脆李、青檀表现出对大部分营养元素具有较高的循环利用养分或保持养分的能力,而香椿及刺槐相对较弱。

In order to assess the change of triploid populus tomentoza pulp plantation long-term-site productivity, The paper studied on effects of aboveground litterfall, fine root turnover and wet dust precipitation in nutrient cycling of triploid populus tomentoza pulp plantations at different ages,namely 2a、4a、5a、6a.It studied influence of different factors on decomposition of leaf、tree bark and twig of triploid populus tomentoza to select the operations to accelerate the decomposition and nutrient release. Finally, it studied influence of different intercrops on plantation site productivity and the relationship of intercrops and triploid populus tomentoza to select suitable intercrops. The main results as follows:(1)The aboveground litterfall of triploid populus tomentoza increased along with age from 216.03±59.7gm~(-2) at 3a to 482.38±101.3gm~(-2) at 7a, The N returned by litterfall wasl8.38±2.46kg.hm~(-2)a~-121.63±2.25kg.hm~(-2a~-139.51±4.61kg.hm~(-2a~-138.89±4.89kg.hm~(-2a~(-1) at 3a、5a、6a、7a respectively. The P returned by litterfall was 5.80±0.62kg.hm~(-2)a~(-1)、8.16±0.94kg.hm~(-2)a~(-1), 11.31±1.33kg.hm~(-2)a~(-1)、11.76±1.37kg.hm~(-2)a~(-1) at 3a、5a、6a、7a respectively. The nutrient returned by fine root turnover increased along with age, too. The N returned by fine root turnover was 3.85±0.41kghm~(-2)a~(-1)、5.22±0.63kghm~(-2)a~(-1),7.62±0.89kghm~(-2)a~(-1),9.17±1.22kghm~(-2)a~(-1) at 3a、5a、6a、7a respectively. The P returned by fine root turnover was 0.73±0.07kghm~(-2)a~(-1)、1.69±0.09kghm~(-2) a~(-1)、1.92±0.31kghm~(-2)a~(-1)、1.96±0.21kghm~(-2)a~(-1) at 3a、5a、6a、7a respectively. The leaf was the principal pathway to return nutrient to soil among litterfall, fine root turnover and wet dust precipitation. The proportion of returned N by leaf was 74.84%、71.96%、78.58%、75.03% at 3a、5a、6a、7a respectively,The proportion of returned P by leaf was 85.93%、80.31%、83.04%、83.23% at 3a、5a、6a、7a respectively. Therefore, it is important to protect and utilize the leaf in order to maintenance and enhance the long-term-site productivity of triploid populus tomentoza pulp plantation.

本文采取时序研究法,以3a、5a、6a、7a共4个不同年龄的三倍体毛白杨纸浆林为对象,研究了地上凋落物、细根周转、湿沉降在林分N、P营养元素循环中的作用及不同年龄林分N、P营养元素循环的特征,以评价三倍体毛白杨纸浆林长期立地生产力的变化;采取网袋法研究了不同因素对落叶、树皮、树枝分解的影响,以确定加快其分解、促进养分释放的措施:同时研究了不同间作物对林地影响、林木与间作物之间关系,以选择能维持立地生产力的合适的间作物种类等内容,得到以下结论:(1)随着年龄的增加,三倍体毛白杨地上凋落物的数量从3a的216.03±59.7gm~(-2)增加到7a的482.38±101.3gm~(-2),通过凋落物归还的N分别为:3a时为18.38±2.46kg.hm~(-2)a~(-1),5a时为21.63±2.25kg.hm~(-2)a~(-1),6a时为39.51±4.61kg.hm~(-2)a~(-1),7a时为38.89±4.89kg.hm~(-2)a~(-1),归还的P分别为:3a时为5.80±0.62kg.hm~(-2)a~(-1),5a时为8.16±0.94kg.hm~(-2)a~(-1),6a时为11.31±1.33kg.hm~(-2)a~(-1),7a时为11.76±1.37kg.hm~(-2)a~-1随着年龄的增加,通过细根周转归还的养分也在增加,归还的N分别为:3a时3.85±0.41kghm~(-2a~(-1),5a时5.22±0.63kghm~(-2)a~(-1),6a时7.62±0.89kghm~(-2)a~(-1),7a时9.17±1.22kghm~(-2)a~-1归还的P分别为:3a时0.73±0.07kghm~(-2a~(-1),5a时1.69±0.09kghm~(-2)a~(-1),6a时1.92±0.31kghm~(-2)a~(-1),7a时1.96±0.21kghm~(-2)a~-1从地上凋落物、细根周转、湿沉降三种不同途径归还林地养分所占的比例来看,地上凋落物中的落叶是归还养分主要途径,年龄在3a、5a、6a、7a时,通过落叶归还的N所占比例分别为74.84%、71.96%、78.58%和75.03%,归还的P所占比例分别为85.93%、80.31%、83.04%和83.23%。

Every group rats were divided into two parts with the same rats. At last the rats were redivided ,then them were killed and their brains were excluded after one hour.4 hours or one week.Results PO2 and cerebral blood flow of E-selectin 2mg/kg group were higher than that of saline and lower than that of l0mg/kggroup(p.01).The volume of cerebral infarct and blood speed of E-selectin 2mg/kg group was smaller than that of saline and larger than that of l0mg/kg group(p.01).At the same time PCh.speeds.flows and the volume of infarct were related with the time (p.01).Conclusion E-selectin can effectively increase PC2 and rCBF after ischemia and reperfusion .reducing infarct volumes and speeds of blood at the same time . Artificial synthetic E-selectin can effectively reduce the reperfusion injury following cerebral ischemia.

结果 生理盐水组,2mg/kg组,10mg/kg组PO_2值依次提高(p<0.01),脑皮质血流量依次提高(p<0.01);脑血流速度依次减低;脑梗死体积依次减少(p<0.01);同时在测定的时间段里PO_2和脑组织血流量值与时间成正相关(p<0.01);脑梗死体积和脑组织血流速度值与时间成正相关(p<0.01)。E一选择素在脑缺血再灌注损伤中表达的实验研究中文摘要结论E一选择素能增加脑缺血再灌注损伤的脑组织氧饱和度,脑血流量,减低脑血流速度;减少脑损伤体积;从而保护了脑缺血再灌注损伤的脑组织。

The experimental results indicated that the fructose (P=68.7%) is the most significant factors, the next factor is shaking time (P=15.2%), the third factor is non-dairy creamer (P=13.5%) and last factor is taros power (P=2.6%).

实验结果显示,在影响新型泡沫摇摇机摇动的四个参数中,果糖(P=68.7%)影响程度最大,其次为摇动时间(P=15.2%),第三影响参数为奶精(P=13.5%),最后则为芋头粉(P=2.6%)。

The number of abnormal structure of thyroid gland of B group was significantly higher than that of A and D groups(P .05).The abnormal number by blood biochemistry detection of A, B, C group was significantly higher than that of D group(P.05). Conclusions : The thyroid gland volume and blood biochemistry abnormal number of patients of type 2 diabetes was obvious increase than normal control group.

结果:A组、B组、C组甲状腺体积较D组明显增加(P.05),B组甲状腺体积较A组、C组明显增加(P.05);B组甲状腺肿大例数较A组、C组、D组明显增多(P.05);C组甲状腺结构异常例数较A组、B组、D组明显增多(P.05),B组甲状腺结构异常例数较A组、D组明显增多(P.05);A组、B组、C组的血液生化指标异常数较D组明显增多(P.05)。

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