查询词典 Of Human Bondage

There is still no consensus on the expression of the two isoforms in the human prostate and their cellular locations are also controversial. It is not known to which extent each of these isozymes contributes to overall enzyme activity in different prostatic tissues. In an attempt to elucidate which isoform of the 5α-reductases is responsible for the metabolism of T in normal and diseased human prostate, we performed qualitative and semi-quantitative studies of both enzymes in normal, benign hyperplastic and adenocarcinomatous human prostate tissues by RT-PCR and Immunohistochemistry methods.

现已成功地克隆出编码Ⅰ型及Ⅱ型5α-还原酶的基因，关于这两型5α-还原酶在前列腺组织及不同类型细胞中的存在、分布情况及其对组织中睾酮代谢各自所起的作用仍存在许多争议，为此我们应用RT-PCR及免疫组化方法对国人正常、良性增生以及前列腺癌组织中两型5α-还原酶的表达进行了定性和半定量研究。

Another one is the lack of accurate understanding of operator "s need and proper administrable structure. Under the condition of market economy, operator"s incentive payment should sufficiently reflect human capital deposit, human capital price, human capital value as well as operators needs. The author points out this is the essential way to resolve the problem.

在市场经济条件下，经营者的激励报酬必须充分考虑经营者人力资本存量、市场定价，充分考虑经营者的人力资本价值及经营者的实际需要，笔者认为这些是解决我国国有企业经营者薪酬激励机制问题的基本思路。

The authers fetched the embryo calvarial peristeum tissue, got human osteoblast by enzyme-assimilating methods and tissue-block culture methods. We observed the morphological change, growth feature and osteogentic capability, of osteoblast during culture in vitro with phase contrast invert microscope, drew the growth curre and identified the cells by alkaline phosphatase dye. At same time, the morphology and bioactivity of 3-5th-generation osbeoblast and anabiotic cells was studied comparatively. 2. titanium particles were examined by scanning electron and the size was determined by semi-automated image analysis. The 3-5 th gereration of human osteoblast were cultured in medium with different concentration of particulates titanium alloy (1mg/ml, 0.1mg/ml, 0.01mg/ml). Cell growth and proliferation was detected by MTT method after 2、4、6 days that particles were added into medium and ALP activity was measured by kit after 4、7、10 days respectively. 3. With above same methods,the 3-5th generation of human osteoblasts were cultured for 3、6、9days after different concentration of particulates titanium alloy (1mg/ml, 0.1mg/ml, 0.01mg/ml) were added into the medium and OPG gene expression was quantified by RT-PCR.

1、取人胚胎颅骨骨膜，采有用酶消化法和组织培养法获取成骨细胞体外培养并传代，观察细胞形态，生物特点及成骨特性，并绘制生长曲线同时碱性磷酸酶染色鉴定成骨细胞以及比较冻存前3-5 代与冻存后成骨细胞的特点。2、电镜下观察钛合金颗粒的形态并测量其粒径，将不同浓度的钛合金颗粒(1mg/ml，0.1mg/ml，0.01mg/ml)与成骨细胞共同培养，分别于第2、4、6 天用MTT 法测量细胞增殖情况及4、7、10天用试剂盒检测碱性磷酸酶活性。3、分别将不同浓度的钛合金颗粒(1mg/ml，0.1mg/ml，0.01mg/ml)与成骨细胞基因培养3、6、9 天用RT-PCR 方法半定量测定骨保护素基因mRNA 的表达。

Get high purity DCs by Cultured plastic-adherent monocytes isolated from healthy human peripheral blood with GM-CSF and IL-4 for 7 days. To observe the morphology of DCs by inverted phase contrast microscope ,electron microscope and laser confocal microscope. Analyse phenotype of DCs with flow cytometry. Investigate the endocytosis ability of DCs as a group by Horseradish peroxidase endocytosis assay. To appraise allogeneic mixed lymphocytes reaction of DCs by MTT reduction assay. Analyse the levels of IL-12 and TNF in liquids of cultured medium by ELISA and MTT reduction assay respectively. Soluble antigens of HCCs was obtained by 3 freeze-and-thaw cycles. Biological characteristics of HC soluble antigens pulsed DCs were monitored by flow cytometry. According to MTT reduction assay estimated the cell proliferation of self lymphocytes activated by HC antigens pulsed DCs. Get high purity BCG HSP 70 protein by SDS-PAGE electrophoresis and determined its biological activity with ELISA. Analyse phenotype of antigen pulsed DCs primed by BCG HSP70 with flow cytometry. By MTT reduction assay estimated the cell proliferation of self lymphocytes and the MLR of DC based vaccine. Analyse expression of HLA-DR molecule on surface of HCC lines. The IFN-γ mRNA in lymphocytes after actived by DC vaccine and the Fas-L expression on DC and DC vaccine primed lymphocytes were detected by in situ hybridization and flow cytometry respectively. Specific cytotoxity lysis of T lymphocytes and nonspecific inhibition of liquids in culture medium against HCC lines were also tested. Detect expression of hAFP on four HCC lines with Cell-ELISA. Induce apoptosis of HCCs with actinomycin-D. Interaction of DCs and apoptotic cells was observed under transmission electron microscope. Growth inhibition test of DC against HCC lines was also performed. Establish the nude mouse model bearing human HC xenografts and indentify the characteristic of tumour by histochemistry and immunohistochemistry techniques. Prevent and treat transplanted human HC on nude mouse with Freezing and anabiotic HC specific lymphocytes.

用GM-CSF和IL-4从健康人外周血诱导DC；分别用倒置相差显微镜、电子显微镜及激光共聚焦显微镜观察DC形态；流式细胞术检测DC表型；HRP吞噬实验测定DC的群体内吞能力；MTT法检测同种异体混合淋巴细胞反应；ELISA法和MTT法分别测定DC培养上清液中IL-12和TNF水平；冻融法制备肝癌细胞可溶性抗原；流式细胞术检测负载肝癌可溶性抗原后DC的生物学特性；MTT法检测DC负载肝癌抗原后对自身淋巴细胞增殖的影响；SDS-PAGE制备电泳纯化BCG HSP70并鉴定纯度，ELISA测定活性；流式细胞术检测负载抗原DC经BCGHSP 70活化后的表型；MTT法检测肝癌DC疫苗对自身淋巴细胞增殖的影响和混合淋巴细胞反应；流式细胞术检测肝癌细胞表面HLA-DR表达；MTT法检测肝癌DC疫苗对自身淋巴细胞的活化；原位杂交法检测肝癌DC疫苗活化后的淋巴细胞IFN-γmRNA表达；流式细胞术检测DC和肝癌DC疫苗活化后淋巴细胞表面Fas-L；MTT法分别检测肝癌DC疫苗活化的淋巴细胞和其培养上清对肝癌细胞的特异性杀伤和非特异性抑制作用；Cell-ELISA检测人肝癌细胞hAFP表达；MTT法检测负载AFP表位肽和凋亡肝癌细胞DC对自身淋巴细胞增殖的影响；ELISA法和MTT法分别测定活化后淋巴细胞培养上清中TNF和IL-12水平；肝癌细胞凋亡的诱导和检测；DC吞噬凋亡肝癌细胞后的电子显微镜观察；DC对肝癌细胞的生长抑制试验；人肝癌裸鼠皮下移植瘤动物模型的建立及其组织学和免疫组织化学鉴定；DC及肝癌特异性淋巴细胞预防和治疗人肝癌裸鼠皮下移植瘤；冻存和复苏后的肝癌特异性淋巴细胞预防和治疗人肝癌裸鼠皮下移植瘤。

Desire is human only if the one desires, not the body, but the Desire of the other … that is to say, if he wants to be 'desired' or 'loved,' or, rather, 'recognised' in his human value … In other words, all human, anthropogenetic Desire … is , finally, a function of the desire for recognition.

如果我们把理性视为他自身与它的对方，把它固定在这个对立的个别环节里，那麼这就是非理性的了解理性，而这种对立的诸环节越是纯粹，这种只为意识所自觉或者只由意识坦率表述出来的内容，外表就越是荒唐。

The advantage of an anthropomorphically shaped being was that it could be a truly general-purpose robot, able to utilize human tools; navigate doors, stairs and environments designed for humans; use human gestures; and ideally perform any task a human could perform.

这么做的好处是它可以是一个真正一般用途的机器人，能够使用人类工具；通过门、上下楼梯，在人类设计的环境中活动；使用人类的手势；理想地进行任何一个人类能够进行的任务。

Human beings appose each other on a great many issues, nations may disagree about systems of governments and human relations may be played by ideological factions that we all share the ability to laugh and laughter in turn depends on their most complex and subtle of all human qualities, a sense of humor.

人类在许多问题上势不两立，国与国之间对政体的看法可能大相径庭，人际关系可能带有意识形态的烙印。但是我们都有笑的能力，而笑声赖以存在的基础却是人类所有品质中最复杂最含蓄的部分，即幽默感。

Human beings appose each other on a great many issues, nations may disa gree about systems of governments and human relations may be played by ide ological factions that we all share the ability to laugh and laughter in turn dep ends on their most complex and subtle of all human qualities, a sense of hum or.

人类在许多问题上势不两立，国与国之间对政体的看法可能大相径庭，人际关系可能带有意识形态的烙印。但是我们都有笑的能力，而笑声赖以存在的基础却是人类所有品质中最复杂最含蓄的部分，即幽默感。

Human beings appose each other on a great many issues, nations may disagree about systems of governments and human relations may be played by ideological factions that we all share the ability to laugh and laughter in turn depends on their most complex and subtle of all human qualities, a sense of humor.

欢迎诸位光临西门子重庆公司的揭牌典礼，各位愿意在此与我们一起度过西门子公司历史上的这一重要日子，我感到万分荣幸。

Human beings exccssively artificialize and symbolize the world through limitless inspirations and imaginations, which makes people deeply indulged in the false images created by ourselves. The structure and characteristics of these false images reversely change everything that human beings and the society rely on. Consequently, questions and perplexity on meanings and values of the society are raised by human beings due to those false images.

在想像世界的无限激发上，人类过度地人工化与符号化这个世界，这不但使得人类深陷於由其本身所制造的虚假意象里，也因为这些虚假意象的结构与特质，反身过来改变了人类与社会生活的一切性质，也进而成为人类对於这个社会在意义与价值之间的质疑与茫然，我们竟无法去依存眼前所见的事实。

Nowadays, most of research are to build a transmutative Petri Nets through adding controlling place sets, controlling arc sets and controlling policy to the basic Petri Nets, while the Controlled Petri Nets could be used to argue many controlling theory problems conveniently and to induce many logically and physically supervisory and solve the Event Feedback Controlling Problems and State Feedback Controlling Problem in DEDS supervisory theory.

目前大多数的研究表现为在变形后的受控Petri网基础上，利用各种方法求得各种逻辑型、结构型控制器，解决DEDS监控理论中的事件反馈控制问题与状态反馈控制问题。

On one hand, there are discussions with the works council and union about extension of short time working up to the end of September.

一方面，有讨论，工程理事会和联盟关于延长工作时间短至9月底。