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The high identity of hira gene ORF in gibel carp and colored crucian carp indicates that the function distinction may be caused by the expression distinction regulated by regulation region of gene.

由于彩鲫是两性生殖方式鱼类，在受精前hira基因大量表达，可能为受精时

In the research, one kind of guzmania species named ostara was employed to be as material, and the full-length cDNA plasmid library of floral organ was constructed successfully by using SMART technology. The library had 3×10^6 original titer and more than 1 kb insert fragments. After 5'EST sequencing from 2004 positive clone chosen at random and clustering analyse, 1 758 high-quality sequences and 1 365 unigenes including 175 contigs and 1 195 singlets were obtained. These unigenes had 1 283 valid ORFs. COG analysis showed that those proteins coded by EST sequences were divided into 22 classes. Through blast analysis, full length or part cDNA of some genes controlling flower color, development of floral organs, florescence regulation and other breeding value genes were obtained.

本研究以擎天凤梨属品种Ostara为材料，采用SMART技术成功构建了擎天凤梨花器官的质粒型全长cDNA文库，初始文库滴度为3×10^6，插入片段平均长度大于1kb；随机挑取2004个阳性克隆进行5'EST测序，获得高质量序列1758条，经拼接获得1365条单基因簇，其中跨叠群175个和单条序列1195个；经ORF寻找共获得有效ORF1283条；经COG分析EST序列编码的蛋白质被分为22类；经Blast分析后，获得一批花色、花器官发育、花期调控以及其它育种价值基因（包括cDNA全长或片段）。

KDa. The deduced amino-acid sequence of the ORF showed high similarity to those of the above △〓-fatty acid desaturases which had characteristics of membrane-bound desaturases including three conserved histidine-rich boxes and two long hydropathy profile.

序列分析结果表明其具有一个长度为1377bp、编码458个氨基酸的开放阅读框，所编码蛋白质的分子量为52.3kDa。

To study the function of this ORF, we constructed Tn5gusA5 insertional and integrational mutants in the corresponding gene of wild type strain 8004 and analyzed the phenotypes of the mutants.

为了研究该σ因子在Xcc中的作用，我们构建了该基因的Tn5gusA5的插入突变体和pK18mob定点整合突变体并进行了表型分析。

Our previously study has been showed that transgenic N.benthamiana and N.tabacum plants expressing TYLCCNV DNAβC1 developed pleiotropic morphologic abnormalities such as leaf curling,distortion and interveinal protuberances on the abaxial surface. To determine whether theβC1 protein of TbCSY is also responsible for symptom induction,we prepared transgenic N.benthamiana and tomato plants expressing the TbCSV DNAββC1.Of the rooted plantlets,only 10%of transgenic N.benthamiana plants were moderately abnormal,including upward leaf curling.

DNAβ互补链上编码一个保守的ORF，即βC1，对TYLCCNV DNAβ的βC1的突变发现它编码一个症状决定因子，表达TYLCCNV DNAβ的βC1的转基因烟草产生曲叶、畸形和叶背面组织增生等发育异常的表型，而大部分转化TbCSV DNAβ的βC1基因的烟草和番茄植株均与正常植株类似，只有少数烟草表现出轻微叶上卷表型。

Signal peptide sequence was removed lest it couldnt be recognized by Bacillus subtilis, gene coding for mature peptide was ligated to downstream of sacB signal peptide sequence of pWB980 to form a new ORF and generate pWB980-LipA, gene coding for its chaperone was also amplified and ligated to the downstream of LipA to generate a secretion/expression vector pWB980-LipAB.thechaperone gene was sequenced and analyzed by multiple alignments, resulted showed that there were mutations of nucleotides(1～7) and amino acids (1～2) with the other reported genes.

为了防止枯草芽孢杆菌信号肽酶不能识别脂肪酶的信号肽，切除掉脂肪酶的信号肽编码序列，与枯草芽孢杆菌分泌表达质粒pWB980连接并与质粒上的SacB信号肽构成一个完整的开放式阅读框，获得重组质粒pWB980-LipA，因为该脂肪酶的活性表达需要一个特异性分子伴侣帮助折叠成有活性的构象，将脂肪酶分子伴侣基因串连到脂肪酶基因的下游，获得重组分泌表达载体pWB980-LipAB并且测序分析分子伴侣的基因序列和氨基酸序列并与其它报导的序列进行了比对，发现有1到7个碱基的差异和1到2个氨基酸残基的突变。

RbcL gene was obtained by polymerase chain reaction and cloned into pDM19-T vector. The positive clone identified by PCR was sequenced. The nucleotide sequence was 1266 bp long, including an ORF of 1031bp, encoding a putative 343 amino acids. The deduced amino acid sequence had a high identity above 94.13% with that of tobacco, spinach, sweet potato, corn, Arabidopsis thaliana, Oryza sativa, Vitis vinifera, Marchantia polymorpha and Citrus paradise.

阳性克隆鉴定后进行测序，序列分析结果表明：该基因片断长为1266 bp，包括1031 bp的编码区序列，编码343个氨基酸；其编码区氨基酸与烟草、菠菜、玉米、甘薯、拟南芥、水稻、葡萄、地钱和葡萄柚等9个物种的同源性94.13%以上，并构建了它们间的亲缘关系树。

An analysis of the ORF and its deduced amino acid sequence were performed with DNAStar Programs.

这可能与细胞本身的密度和对PRV的易感性有关。

We can not keep ORF Built to Last?

我们能不能保持欧尔福的基业长青？

The results of sequence analysis shown that the ORF of MP gene is 804bp.encode 268 amino acids .

将其克隆到pMD-18-T vector上，得到含有完整MP基因的重组质粒pTMP-D。

The labia have now been sutured together almost completely.The drains and the Foley catheter come out at the top.

此刻阴唇已经几乎完全的缝在一起了，排除多余淤血体液的管子和Foley导管从顶端冒出来。

To get the business done, I suggest we split the difference in price.

为了做成这笔生意，我建议我们在价格上大家各让一半。