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The ORF of profilin of Caryota mitis pollen was amplified by RT-PCR using specific primers and cloned into the expression vector pET-28a. The recombinant Caryota mitis pollen profilin was expressed in Escherichia coli BL21 (DE3). The recombinant protein was purified by affinity chromatography using Ni(superscript 2+) coupled sepharose.

然后设计带有酶切位点的特异性引物，采用RT-PCR获得整个短穗鱼尾葵花粉profilin的开放阅读框，将其与pET28a载体连接并转化大肠杆菌BL21(DE3)进行诱导表达，通过Ni(上标 2+)亲和层析柱对重组蛋白进行纯化，采用Western-blot检测其IgE结合活性。

Initial sequence data for Pilchard herpesirus was acquired by comparing the highly consered region of the ORF 62 gene of other piscine herpesiruses (Ictalurid herpesirus 1 and Salmonid herpesirus 2 [Oncorhynchus masou irus, SaH2, OM]), and designing primers that successfully amplified a fragment of PH.

扩增产物的序列分析不同于其它已知的疱疹病毒，因而也可以用于区别沙丁鱼疱疹病毒

Initial sequence data for Pilchard herpesvirus was acquired by comparing the highly conserved region of the ORF 62 gene of other piscine herpesviruses (Ictalurid herpesvirus 1 and Salmonid herpesvirus 2 [Oncorhynchus masou virus, SaHV2, OMV]), and designing primers that successfully amplified a fragment of PHV.

扩增产物的序列分析不同于其它已知的疱疹病毒，因而也可以用于区别沙丁鱼疱疹病毒文题：Molecular biology of channel catfish virus

ABSTRACT］ObjectiveTo construct the prokaryotic expression vector of tegument protein pp85 of human herpesvirus 7 and to express it.

摘要］ 目的构建人疱疹病毒7型(HHV7)开放读码框（ORF U14的原核表达载体，并且进行原核表达。

A German tourist who injured himself while "skiing" in the Tyrolean Alps this month said he was asked to swipe his bank card before he could be treated, local radio ORF reported on Friday.

当地电台「奥地利广播公司」周五报道，一名本月在提洛尔州阿尔卑斯山脉「滑雪」时受伤的德国游客说，他被要求刷银行卡，才可获得治疗。

It was known that ORF of SeMNPV egt was 1572bp long and encodes 523 amino acids through DNAstar software analysis. TATA boxes lies at 48~53 and 68~72bp of the 5'-nonencoding region upstream of a translational start site. Comparing with seven kinds of nuclear polyhedrosis virus and one kind of granulosis virus, it indicated that the SeMNPV egt gene is the highest match the Agrotis setegum nuclear polyhedrosis virus.It has 75% nucleotides and 79% amino acid identity.

采用DNAstar软件分析，与7种核多角体病毒及1种颗粒体病毒egt基因的同源性比较显示，SeMNPV egt基因与核多角体病毒egt序列同源性较高，其中与黄地老虎核型多角体病毒同源性最高，核苷酸和氨基酸序列同源性分别为75%和79%。

In a small number of influenzas, including all S-OIVs, the PB1-F2 ORF is truncated by termination codons at positions 12, 58 and 88, and its absence is associated with avirulence in mice.

目的是使猪源流感病毒对某些特定种族无毒，而对其它种族有毒？

Multiple sequence alignment and phylogenetic analysis of CVX-NTU and other completely sequenced potexviruses demonstrated that CVX-NTU has a close relationship with potexviruses infecting plants in the family Cactaceae.

将CVX-NTU病毒分离株各个ORF与其它已知序列的同属病毒作胺基酸序列比对及类缘分析，结果显示感染仙人掌科的potexvirus都属同一个分群中，显示彼此的类缘关系较密切。

BADH cDNA (1785bp) included a 63 bp 5 "UTR, a 219 bp 3 "UTR and a 1503 bp ORF encoding a 500-amino-acid polypeptide which showed closely similar sequence identity to BADH from Chenopodiaceae.

将BADH的编码区推测的氨基酸在GenBank中进行分析，能找到磷酸果糖激酶、乙醛脱氢酶家族，谷胺酰磷酸还原酶等的保守区域。

Full-length cDNA of Gibel carp pou2 gene was cloned from gastrula SMART cDNA library through RACE-PCR. It consists of 2421 bp, with an ORF of 1416 hp encoding a protein of 471 aa. The complete amino acid sequence shares 91.0% identity with zebrafish pou2 gene product.

用RACE-PCR方法从原肠期SMART文库中扩增到银鲫pou2基因的全长cDNA，其全长为2421bp，开放阅读框为1416bp，编码471个氨基酸，与斑马鱼pou2基因的氨基酸序列一致性高达91.0%。

The labia have now been sutured together almost completely.The drains and the Foley catheter come out at the top.

此刻阴唇已经几乎完全的缝在一起了，排除多余淤血体液的管子和Foley导管从顶端冒出来。

To get the business done, I suggest we split the difference in price.

为了做成这笔生意，我建议我们在价格上大家各让一半。