查询词典 ORF

The expression absence of LRRC4 was ascribed to the loss of homozygosity of 7q32-ter in U251 cell lines. Conclusion The expression of LRRC4 gene is absent in glioblastoma cell lines, and it offers the important experiment proof for LRRC4 to act as a new candidate of brain tumor suppressor gene from glioma.

通过对胶质瘤细胞系基因组DNA中LRRC4的ORF序列的PCR扩增和测序分析，发现SF126，SF767，M17细胞中，ORF序列第279位氨基酸的第3个密码子存在同义点突变(3/5)，而U251和U87细胞基因组DNA中发生了LRRC4基因的缺失突变(2/5)。

We find that the "spurious" ORFs (IHI 14) distributed mainly in three classes, namely, in the class of "similarity to unknown proteins","no similarity" and "questionable ORFs".

我们发现，虚假的ORFs(IHI≤14)主要分布在三类ORF中，即：和未知蛋白相似的ORF，无相似的ORF，以及可疑的ORF。

3 The inhomogeneity rule-coding and non-coding ORFs differ in inhomogeneity of base composition on three codon positions. By use of inhomogeneity index one can make a distinction between coding (IHI＞14) and non-coding (IHI≤14) ORFs at 95％ accuracy.

遵守这一规则的ORF在酵母基因组中占99.7％。3ORF碱基分布的非均匀规则—ORF的编码能力和密码子三个位点上四种碱基的组成的不均匀性相关。

Sequence analysis showed that the full-length cDNA sequence of Sc-SAMDC (GenBank Accession number: GQ246459) was 1 968 bp, with three open reading frames, tiny ORF, upstream ORF and main ORF. The mORF was 1200 bp encoding 399 amino acid residues with a molecular mass of 43.6 kD, and the deduced protein had two highly conserved function domains (proenzyme cleavage site and PEST domain).

序列分析显示，甘蔗Sc-SAMDC基因(GenBank Accession number： GQ246459) cDNA全长1968 bp，存在3个读码框（袖珍读码框tORF、上游读码框uORF和主读码框mORF），mORF长1200 bp，编码399个氨基酸的SAMDC酶原，预测分子量为43.6 kD，该酶原含有两个高度保守的功能结构域（酶原剪切位点和PEST结构域）。

The results of sequencing show that GTPV P32 gene was 969bp and encode 323 amino acids, Sheep pox virus P32 gene was 972bp and encode 324 amino acids, FGD-CPV P32 gene was 969bp and encode 323 amino acids.

结果表明：GTPV P32基因开放阅读框全长969bp，编码323个氨基酸；绵羊痘病毒(Sheep pox virus，SPPV)P32基因ORF全长972bp，编码324个氨基酸；羊胎皮肤细胞分离毒P32基因ORF全长969bp，编码323个氨基酸。

This ORF included 1101bp, encoding 367 amino acids, 92% similar to human and mouse.

结果分离的ORF全长1101bp，编码367个氨基酸，与人鼠氨基酸序列92%同源；利用生物信息学软件分析出此蛋白的理化特性并预测了其一级、二级和高级结构。

There was no identical proteins in the database we searched, but it shared high sequence homology with PNZIP from Pharbitis nil and two other open reading frames of unknown function from Synechocystis spp and Porphyra purpurea. These together defined a new family of evolutionarily conserved leucine zipper proteins, which might play a role as a transcription factor in the regulation of gene expression.

GenBank数据库搜寻结果显示，AT103未与任何已知功能基因有同源性，但与裂叶牵牛PNZIP、集胞藻的一个ORF、紫菜的一个ORF，构成了一个在进化上非常保守的含有亮氨酸拉链结构域的新家族，极有可能是一新的核基因转录因子。

Restriction map of H. polymorpha CBS 1976 chromosomal locus containing △9-fatty acid desaturase gene was constructed based upon the data of Southern analysis, and a 3. 4kb BamHⅠ-XhoⅠ genomic fragment was isolated. Sequence analysis showed that it contains an ORF of 1353bp with high homology with cloned yeast △9-fatty acid desaturase genes.

构建了H.polymorpha CBS 1976染色体△9-脂肪酸去饱和酶基因座位的限制性酶切图谱，进而分离了3.4kb BamHⅠ-XhoⅠ基因片段并进行全序列分析，结果表明这个片段含1个与已克隆的酵母△9-脂肪酸去饱和酶基因高度同源的、由1353bp组成的ORF。

The results indicated that whole H-OLE1 gene could complement fad1 mutation in H. polymorpha recipient lacking △9-fatty acid desaturase. However UFA requirement that Saccharomyces cerevisiae ole1 mutant displays was complemented only by ORF of H-OLE1 driven by S.

发现完整的H-OLE1基因可互补缺乏△9-脂肪酸去饱和酶活性的多形汉逊氏酵母营养缺陷型fad1突变体，却不能互补相应的酿酒酵母ole1突变体，而由酿酒酵母GAP表达框架和H-OLE1 ORF组成的嵌合基因可互补上述ole1突变体。

Nested PCR analysis and southern hybridization analysis showed that no polymorphism between H.villosa, Pm97034 and susceptible parent Wan7 107 was detected with the clones from 6VS arm, whereas three clones from H.villosa genome DNA: RH42, RH55, and RH66 showed polymorphism. RGA6 cloned from H.villosa genome DNA was characterized identity to NBS, and show high homology to resistance genes as RPM1 and RPP13 in Arabiadopsis, LRR19 in wheat, and I2C-1 in tomato.cDNA library constructed from T.aestivum-H.villosa translocation line Pm97034 was screened by hybridization with RGA6. Four positive cDNA clones were obtained. R3-2-2 showing 60-90% identity with eukaryotic sulfite oxidases contained an open reading frame of 647bp encoding 140 amino acid, and contained a conserved Moco-dimer domain in the ORF. R6-2-2 showed an ORF containing an Euk-porin domain of 279aa with 20-40% identity to the eukaryotic voltage-dependent anion channel proteins. R8-1-1 showed a complete ORF of 1810bp encoding 493aa with 80-90% identity to plant catalases. R9-1-1 showed an ORF containing a BTB/POZ conserved domain of 204aa with 30-70% identity to pox virus and zinc finger proteins. R3-2-2 and R9-1-1 were the first cDNA clones containingconserved domain of SO and POZ respectively isolated from wheat. R8-1-1 contained a complete ORF with 81% identity to Cat-3. Aspects of the role of R8-1-1 may be same with Cat-3, and it would offer the opportunity for improvement of stress tolerance of wheat.

以RGA6为探针，筛选用抗白粉病小麦—簇毛麦易位系Pm97034构建的cDNA文库，得到了4个阳性克隆。R3-2-2与动植物的亚硫酸氧化酶(sulfite oxidase，SO)有60～90％的同源性，长度为647bp，编码140个氨基酸，具有开放阅读框并含有保守域Moco-dimer.R6-2-2与真核生物的VDAC(voltage-dependent anion chennel)蛋白有20～40％的同源性，长度为1047bp，编码279aa，是一个不完整的开放阅读框，预测结构具有Euk-porin结构域。R8-1-1与植物中已经克隆的过氧化氢酶有80～90％的同源性，长度为1810bp，编码493aa，具有完整的开放阅读框和过氧化氢氧化酶保守域。R9-1-1与动植物的POZ(pox virus and zinc finger protein)蛋白有30～70％的同源性，长度为1446bp，具有开放阅读框和BTB／POZ保守域。R3-2-2与R9-1-1是首次从小麦中克隆到的具有SO和POZ保守域的cDNA序列。R8-1-1具有完整的开放阅读框，与玉米Cat-3基因具有81％同源性，预测R8-1-1可能具有与Cat-3类似的功能，可为转基因小麦抗逆育种提供新的基因。

The labia have now been sutured together almost completely.The drains and the Foley catheter come out at the top.

此刻阴唇已经几乎完全的缝在一起了，排除多余淤血体液的管子和Foley导管从顶端冒出来。

To get the business done, I suggest we split the difference in price.

为了做成这笔生意，我建议我们在价格上大家各让一半。