查询词典 ORF

In this paper, the cDNA sequence from Ampullaria crossean stomach named Dy1300 was obtained by RT-PCR firstly and cloned into vector pMD 18-T. Its ORF named SXYN contains 882bp nucleotide encoding a 294 amino-acid protein. The percentage of similarity of SXYN with the reported cellulase EGX gene sequence from Ampullaria crossean was 89.8%.

本研究首先通过RT-PCR的方法从福寿螺胃中扩增出新的纤维素酶cDNA序列―Dy1300，并与克隆载体pMD 18-T相连接，通过对其全序列的分析，确定其开放阅读框ORF长度为882bp，命名为SXYN(GenBank， AY941794)，推定的氨基酸序列含294个氨基酸，与已报道的福寿螺纤维素酶C端基因的同源性达89.8%。

This ORF encodes a 640-amino-acid polypeptide which shares 95%, 71%, 71%, 70% and 32% homologies to p73 of Barbul barbus, Mus musculus, Homo sapiens, Cercopithecus aethiops and Mya arenaria, respectively.

此ORF可转译出640个胺基酸，经由比对结果发现，斑马鱼的p73胺基酸与其它物种的相似度为：与人为71﹪，与猿猴为70%，与老鼠为71%，与鲶鱼为95%，与蜗牛为32%。

In a small number of influenzas, including all S-OIVs, the PB1-F2 ORF is truncated by termination codons at positions 12, 58 and 88, and its absence is associated with avirulence in mice.

在少数的流感，包括所有的S– OIVs，PB1 - F2 ORF在位置12，58和88被终止密码子截断，这种截断造成的缺乏与小鼠实验无毒性相关。

In a small number of influenzas, including all S-OIVs, the PB1-F2 ORF is truncated by termination cod** at positi** 12, 58 and 88, and its absence is associated with avirulence in mice.

在少数的流感，包括所有的S– OIVs，PB1 - F2 ORF在位置12，58和88被终止密码子截断，这种截断造成的缺乏与小鼠实验无毒性相关。

Sequencing of this 4.3kb DNA fragment revealed that there is a β-glucosidase gene urnbgl3A with an ORF of 1956bp, encoding product shared 63% identity and 79% similarity with a beta-glucosidase (GenBank NO:AAX16378.1) of uncultured murine large bowel bacterium BAC 31B, and shared 50% identity and 67% similarity with a beta-glucosidase-related glycosidases (GenBank NO:ZP-00308419.1) of Cytophaga hutchinsonii.

测序结果表明在4.3kb的片段上有一个全长为1956bp的ORF，编码一个可能的β-葡萄糖苷酶基因umbgl3A，其编码产物与一个来源於小鼠大肠未培养细菌的β-葡萄糖苷酶(GenBank Acession NO：AAX16378.1)一致性为63%、相似性为79%；与哈氏噬纤维菌的β-葡萄糖苷酶(GenBank Acession NO：ZP-00308419)的一致性为50%、相似性为67%。

In our study, 3 genotypies have been respectively demonstrated in UL139 and UL149 for the first time, and the corelations between certain genotypical structure and certain diseases were also proposed; The existence of the 3 genotypes of UL144 was foremost verified in isolates from congenital infants; Furthermore, the UL140, UL141 and UL145 genes were observed to be greatly discrepant to those had been described previously: comparing with Toledo, 231 nt are inserted in UL140, 2 more ORFs are obtained in UL141, and the UL145 ORF moves upstream by 90 nt. Except UL144, the sequences of 18 else genes in clinical isolates were submitted for the first time, and 479 sequences were assigned by GeneBank in total. Relevantly, 9 papers were published.

我们在学术界首次证实了HCMV UL139、UL149两个基因在临床分离株中分别存在三种基因型，发现了其中某个基因型的基因结构与特定来源的分离株存在一定的对应关系；首次在先天感染分离株中验证了UL144三种基因型的存在；首次证实了UL140、UL141和UL145等基因与原先认识的基因结构不同，其中UL140基因较Toledo株增加了231个碱基、UL141产生2个新的基因编码区、UL145 ORF较Toledo株前移90个碱基；首次或最先提交了除UL144基因外其余18个基因的临床分离株序列，本项目组共有479个HCMV相关基因序列被GenBank收录；发表研究论文9篇。

OrLPT1 ORF encods a polypeptide with 535 amino acid residues (molecular mass of 57.6KDaltons), which shares more than 64% amino acid identity with some other high-affinity Pi transporters of plants. Hydropathy analysis of the deduced polypeptides showed that the OrLPT1 is highly hydrophobic and contains 12 putative membrane-spanning regions.

OrLPT1 ORF 编码长为535 个氨基酸(分子量57.6 KDaltons)的蛋白，与其他植物物种磷酸盐转运蛋白的同源性在64%以上；多肽链的疏水性分析表明，OrLPT1 具有12 个疏水的跨膜区及高亲和力磷酸盐转运蛋白中保守的3 个磷酸化作用位点：蛋白激酶C作用位点、N端糖基化部位和酪蛋白激酶II 作用部位； 3。

The N gene ORF was then subcloned into pET-30a vector and the recombinant plasmid was transformed into E.coli BL21 (DE3) and induced with IPTG. The protein expression was determined by SDS-PAGE. The expressed protein had a molecular weight of 54.4 Ku that existed as inclusion body. Thin-layer scanning showed that the expression product accounted for 30.5% of the total bacterial proteins. The recombinant protein possessed native biological activity and could react with anti-PEDV hyperimmune serum in Western blot.

以阳性质粒为模板，用分别含有BamH Ⅰ和Xho Ⅰ酶切位点的上、下游引物扩增得到ORF，其PCR产物经BamH Ⅰ和Xho Ⅰ双酶切后定向克隆到pET-30a载体，构建的重组质粒命名为pET-30a-PN；将pET-30a-PN转化到大肠杆菌BL21 (DF3)中，在IPTG诱导下进行表达；SDS-PAGE结果表明表达出与预期大小相符的约54.4 Ku的重组蛋白，重组蛋白以包涵体形式存在；薄层扫描结果表明表达产物占菌体总蛋白的30.5%；Western blot分析表明表达的重组蛋白能与抗PEDV高免血清反应，说明该重组蛋白具有免疫学活性。

ORF predicted results showed that there was only one open reading frame.

ORF预测结果表明，contig5Gm只有一个开放阅读框。

ORF is shipped with a predefined set of DNS blacklist definitions and the list can be extended.

ORF的是发运与一个预定义的设置的DNS黑名单的定义和名单可以延长。

The labia have now been sutured together almost completely.The drains and the Foley catheter come out at the top.

此刻阴唇已经几乎完全的缝在一起了，排除多余淤血体液的管子和Foley导管从顶端冒出来。

To get the business done, I suggest we split the difference in price.

为了做成这笔生意，我建议我们在价格上大家各让一半。