查询词典 ORF

In this passage we analysis the construction properties of gene and ORF in prokaryote, I try to fetch some parameters which can describe the properties of gene.

本文致力于分析DNA序列，找到能够区分蛋白质编码和非编码序列的特征，设计算法提高蛋白质编码基因的识别率。

A pair of primers spanned the ORF of goat IL-18BP gene stemming from sheep poxvirus genome were designed.

在羊痘病毒基因组序列中也可能有编码IL-18BP的同源基因。

After amplying a 2.2kb fragment form the PPV-SC1 RF-DNA,we clone the fragment into pMD 18-T,named pTNSl.The whole sequence which is 1989 bp long was determined by sequencing, including the complete ORF of PPV-SC1 NS1 which encoding 662 amino acids.Alignment of pairs of sequence indicates that there are 98% and 99% similary with other porcine parvovirus strains Kresse and NADL-2, respectively. Multiple sequence alignment discloses that there are a few difference between ppv-scl nsl gene and other ppv nsl gene: A-G at 39nt,T-C at 153nt,A-G at 175nt, A-C at 1117nt, A-C at 1535nt .Alternative codon in ppv-scl nsl have distinctly different frequentfy by codonbias analysis at EMBOSS(http://genopole.toulouse.inra.fr/bioinfo/emboss). Thereis not distinct hydrophobicity and transmenbrane helices in ppv-scl nsl protein. Struction domain anslysis of PPV-SC1 NS1 protein indicate that there are a ATP/GTP-binding site motif A at 398-405,16 Protein kinase C phosphorylation site,21 Casein kinase II phosphorylation site,and 3 cAMP/cGMP-dependent protein kinase phosphorylation site.At the same time ,there is a same motif between ppv-scl nsl and Poxvirus D5 protein-like which may share in the same fuction which is necessary during virion duplication.

将PPV-SC1 NS1序列与其他PPV NS1基因进行多序列比对，结果显示，PPV-SC1 NS1与其他的PPV NS1的同源性较高，仅存在个别的差异，分别是第39位A→G，第153位T→C，第175位A→G，第1117位A→C，第1535位A→C；同源搜索比较表明，PPV-SC1与PPV NS1同源性可达98％、99％，与其他的细小病毒NS1基因也存在很大的保守性；密码子偏向性分析结果表明PPV-SC1 NS1基因在同一氨基酸的不同密码子的选择上存在一定的偏向性；PPV-SC1 NS1蛋白总体上说具有亲水性不存在明显的疏水性区段，用swiss TMPRED软件预测PPV-SC1 NS1的跨膜区，返回的结果并没有得到有显著意义的跨膜区的存在；根据基于motif数据库的结构域预测，PPV-SC1 NS1的第393-415位氨基酸残基存在潜在的ATP／GTP结合位点，该蛋白还存在16个蛋白激酶C磷酸化位点，21个酪蛋白激酶2磷酸化位点，3个cAMP-／cGMP依赖蛋白激酶磷酸化位点，PPV-SC1 NS1蛋白与POX_D5(痘病毒D5蛋白)具有一致的保守结构域，推测NS1可能与POX_D5有类似的功能。

The functional allele at the W locus is Ipmyb2, which was newly found in Chinese populations, and it differs mainly in the intron regions from those of the reported allele Ipmyb1; in comparison to Ipmyb2, the newly found second allele ipmyb2 has eight substitutions in the exons, a 6-bp deletion, and a 19-bp deletion, causing ORF shifting, and exists mostly in white-flowered individuals.

以上结果表明：1花青素代谢途径上酶基因的位点均有一定程度的多态，其中尤以ANS为最高；调控基因ipmyb2的核苷酸缺失造成该基因停止功能，使其纯合子的个体开白色花。2授粉者所介导的花色表型选择确实在中国种群中发生，有可能进一步增加花色代谢途径上基因位点的遗传变异。

Longan1 expressed from the embryogenic callus stage to the globular embryoid stages. Longan2 expressed in the whole process of longan somatic embryogenesis, which was probably a housekeeping gene. Longan3 expressed from the globular embryoid stage to the torpedo-shaped embryoid stage. Longan4、longan4'expressed only at the cotyledonary embryoid stage.

碱基序列分析表明，longan1在3'端存在一个开放阅读框，longan2、longan3分别在5'端存在一个开放阅读框，longan1在3'方向，longan2、longan3在5'方向均没有终止密码子，所以可能往相应的方向延伸仍为各自的ORF，因此可以通过RACE或者筛选cDNA文库的办法来获得它们的全长cDNA。

The labia have now been sutured together almost completely.The drains and the Foley catheter come out at the top.

此刻阴唇已经几乎完全的缝在一起了，排除多余淤血体液的管子和Foley导管从顶端冒出来。

To get the business done, I suggest we split the difference in price.

为了做成这笔生意，我建议我们在价格上大家各让一半。