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Horf came to believe in a sort of linguistic determinism which, in its strongest form, states that language imprisons the mind, and that the grammatical patterns in a language can produce far-reaching consequences for the culture of a society.

orf开始相信一种语言决定论，在它的强大的形式和状态下，语言束缚了思想，并且一种语言的语法模式会对一个社会的文化产生深远的影响。

Horf came to believe in a sort of linguistic determinism which, in its strongest form, states that language imprisons the mind, and that the grammatical patterns in a language can produce far-reaching consequences for the culture of a society.

orf开始相信一种语言决定论，在它的强大的形式和状态下，语言束缚了思维，并且一个语言的语法模式会对一个社会的文化产生深远的影响。

Horf came to believe in a sort of linguistic determinism which, in its strongest form, states that language imprisons the mind, and that the grammatical patterns in a language can produce far-reaching consequences for the culture of a society.

orf开始相信一种语言决定论。在它的强大的形式和状态下，语言束缚了思维，并且一个语言的语法式样会对一个社会的文化产生深远的影响。

Horf came to believe in a sort of linguistic determinism which, in its strongest form, states that language imprisons the mind, and that the grammatical patterns in a language can produce far-reaching consequences for the culture of a society.

orf开始相信一种语言决定论。在它的最强大的形式状态之下，语言决定论主张语言禁锢了思想，并且一个语言的语法模式会对一个社会的文化产生深远的影响。

The cDNA was 1 543 bp at length with an open reading frame of 1152 bp, and there were alternative splices in BmCtD.

该cDNA大小为1543 bp，其中ORF长1152bp，同源性分析表明BmCtD与其他物种的CtD具有较高的相似性。

Molecular homology trees were constructed based on the amino acid sequences of the RdRp,CP and MP,and the results showed that TVDV shares much more homologous with the specieses of the genus Polerovirus than other genuses of family Luteoviridae,which indicated that TVDV should be a definitive member of the genus Polerovirus in the family Luteoviridae.

根据三个ORF编码的氨基酸序列构建的分子同源树的分析，也都显示了TVDV与黄症病毒科马铃薯卷叶病毒属病毒的同源性最高，进一步证实了TVDV应该是黄症病毒科马铃薯卷叶病毒属的确定成员。

The sequences were compared with the known sequences in Genbank with BLAST software. P-ACC/M-GGA200 showed 95% homology to a part of AFLP sequence which linked to fusarium head blight resistance gene fragment (AF389881). The P-AGG/M-GGT_(251) has an ORF, and it had homology to a hypothetical protein of Oryza sativa.

根据BLASTn同源性比较，P-ACC/M-GGA_(200)与GenBank中与抗小麦赤霉病连锁的一段AFLP序列(AF389881)同源性达95%；P-AGG/M-GGT_(251)序列中含有一个开放阅读框，根据BLASTp同源性比较，与GenBank中日本栽培稻一未知蛋白(XP 483138)有同源性。

Coli might be distributed in all the gene. The intact Hu-P68 cDNA ORF including 10bp upstreams of ATG were obtained by PCR deletion and reconstruction. The resulted fragment were cloned into transfer vector pBacPAK〓 at Bam HI site and lipofectioned into 〓 cells with Bsu 36I digested genomic DNA of insect baculovirus BacPAK 〓.

利用PCR技术删除人P68 cDNA翻译起始位点上游10bp以外的5'UTR，并引入BamHI位点，经酶切和测序验证后，将此改造的完整P68 cDNA基因编码区装入转移载体pBacPAK〓，用脂转染法将此重组质粒与酶切的昆虫杆状病毒BacPAK〓基因组DNA片段导入昆虫细胞〓中，经同源重组获得了再生病毒。

The provirus had three small ORFs,ie. Si ,52 and S3,with sizes of 153bp(nucleotides 5113-5265),204bp(nucleotides 5279-5482)and 402bp(nucleotides 7245-7646),respectively.

此外，前病毒还有3个小的ORF，S1位于5113～5265位碱基之间，长为153bp；S2位于5279～5 482位碱基之间，长为204bp；S3位于724 5～7646位碱基之间，长为402bp。

We found that neither the fusioned nor the non-fusioned forms of Hu-P68 cDNA open reading frame could express efficiently in the E. coli cells; in different prokaryotic expression vectors and under various promotors resulted in no distinctive variations. Deletion of 5'UTR by synthesis adaptors, deletion of 3'UTR or signal peptide sequence by PCR gained no gross improvement. Only the truncated API-I proteins were observed to be highly expressed when the 3'ORF fragment of the Hu-P68 cDNA was fusioned at the 3'end of PAI-I gene.

结果发现无论是以融合形式存在，还是以非融合形式存在，人的P68 cDNA基因在E.coli细胞中都未能获得高效表达：在不同表达载体中，不同启动子对P68 cDNA基因的表达没有显著差异；利用合成Adapter的方法删除5'UTR，及利用PCR技术删除3'UTR和信号肽序列对表达没有明显改善；分别表达被分割的P68 cDNA5'端编码区、中央编码区和3'端编码区均未见有明显表达；当P68 3'端编码区与高效表达蛋白PAI-Ⅰ基因3'末端融合时，仅见有PAI-Ⅰ以截断形式高效表达出来。

The labia have now been sutured together almost completely.The drains and the Foley catheter come out at the top.

此刻阴唇已经几乎完全的缝在一起了，排除多余淤血体液的管子和Foley导管从顶端冒出来。

To get the business done, I suggest we split the difference in price.

为了做成这笔生意，我建议我们在价格上大家各让一半。