查询词典 O.P.

Results: Seven compounds were isolated from the ethanol extract of the fruits and identified as oleanolic acid, luteolin-7-O-β-D-glucoside, quercitrin,β-sitosterol-3-O-β-D-glucoside, p-hydroxyphenethyl-β-D-glucoside, D-mannitol and hexitol.

结果：从女贞子的乙醇提取物中分离得到7个单体化合物，分别鉴定为齐墩果酸、木犀草素－7-O-β-D-葡萄糖苷、槲皮苷、β-胡萝卜苷、对羟基苯乙醇－β-D-葡萄糖苷、甘露醇及己六醇。

After Cryopreservation of embryo and larvae of pacific oyster Crassostrea gigas, the results indicate the survival rate of trochophore larvae is 61.1% and significantly better than that of other stage embryo or larvae(P.05).The effect of high sucrose density is better than that of low sucrose density with the same DMSO density.

对太平洋牡蛎的胚胎和幼虫的超低温冷冻保存发现，担轮幼虫的解冻复活率最高，达61.1％(3.0M DMSO)，显著高于其他发育阶段幼虫的保存结果(P＜O.05)；牡蛎担轮幼虫的保存结果显示，在相同DMSO浓度条件下，高蔗糖浓度组保存效果好于低蔗糖浓度组，但差异不显著(P＞0.05)。

Results Nine patients with PTE wore performed thromboembolectomy under cardiopulmonary bypass, one patient was performed thromboembolectomy under non-extracorporeal circlulation. One patient died of pulmonary re-embolism postoperatively, utile patients were survival. There was o marked increase of arterial partial pressure of oxygen and oxygen saturation (P.05) and pulmonary arterial systolic pressure decreased significantly (P.05) at the second postoperative week, with significant improvement in quality of life.

结果 9例在体外循环下行肺动脉取栓术，l例在非体外循环下手术。1例围术期死亡，9例存活；术后2周测不吸氧状态下动脉血氧分压、血氧饱和度均有显著改善，与术前比较有显著性差异(P.05)；术后2周超声心动测肺动脉收缩压显著降低，与术前比较有显著性差异(P.05)，生活质量明显改善。

Result: Seven phenolic compounds were identified as rosmarinic acid(1),gallic acid(2),gentisic acid 5-O-(6′-O-galloyl)-β-D-glucopyranoside(3),caffeic acid(4),p-coumaric acid(5),ethyl caffeate(6) and acteoside(7),respectively.

结果：分离得到7种酚类化合物，经结构鉴定为：迷迭香酸(1)，没食子酸(2)，龙胆酸5-O-(6′-O-没食子酰基)-β-D-吡喃葡萄糖苷(3)，咖啡酸(4)，对香豆酸(5)，咖啡酸乙酯(6)和acteoside(7)。

Reaction of 5'-O-p-methoxytrityl-thymidine with 1, 1'-carbonyl-diimidazole gave the 3'-O-carbonylimidazolide, which was condensed in high yield with 3'-O-protected thymidine to give a dithymidine carbonate. In the same way, we obtained the protected trithymidine dicarbonate. After deprotection, we obtained quantatively the trithymidine dicarbonate.By the same stratagy, we completed the synthesis of dithymidine sulfite.It was shown that allyl can be used as a good protection group for hydroxyl of ribose in the synthesis of oligonucleotides consisting in some unstable nucleotide analogues.

我们在第一部分工作的基础上，以烯丙基作为3'-OH的保护基合成了保护的三胸苷碳酸酯以及二胸苷亚硫酸酯，用我们所得的脱保护条件进行了脱保护研究，得到了三胸苷碳酸酯以及二胸苷亚硫酸酯，并研究了它们合成中的一些化学问题，结果表明：烯丙基能很好地运用于这两类寡核苷酸类似物的合成中，其脱保护条件为中性，不会影响碳酸酯键及亚硫酸酯键。

H o nesty is the best p o licy.

诚实是上策。红色的 O 发成 B

Based on relative literatures,this part has included five resesearch aspects as below.1 Fifteen compounds were isolated and purified by extraction,column chromatography,and their structures were determined on the basis of spectral analysis: acteoside(Ⅰ-1),isoacteoside(Ⅰ-2),crenatoside(Ⅰ-3),cistanoside F(Ⅰ-4),sinapoyl-4-O-β-D-glucoside(Ⅰ-5),adenosine(Ⅰ-6),β-siterol(Ⅰ-7),oleanic acid(Ⅰ-8), succinic acid(Ⅰ-9),caffeic acid(Ⅰ-10),protocatechuic aldehyde(Ⅰ-11),p-hydro xybenzyl alcohol(Ⅰ-12),β-daucosterol(Ⅰ-13),D-galacitol(Ⅰ-14),D-mannitol(Ⅰ-15).Ⅰ-4～15 were obtained from this plant for the first time,andⅠ-6,7,9,and 13 were isolated from Orobanche genus for the first time.2 The scavenging test of DPPH showed that most compounds have comparative antioxidant activity as L-ascorbic acid and part of them show better activity such as the O.coerulescens extract and phenylethanoid glycosides.Acteoside showed potent free radical scavenging effects with a median inhibition concentration of 25.6μg/ml.3 The anti-HBV activities of acteoside,isoacteoside and crenatoside were measured,and all of them showed suppressive activity on the expression of HBsAg and HBeAg in the HepG2.2.15 cell line.

本论文在文献调研基础上对紫花列当化学成分及生物活性进行了研究，并从免疫抗病毒角度探讨紫花列当中特征性成分类叶升麻苷的肝保护作用及其机制。1采用大孔树脂、硅胶和Sephadex LH-20等色谱技术对紫花列当进行系统的植物化学研究，从中分离得到19个化合物，利用UV和NMR等波谱手段及理化性质鉴定了其中的15个化合物，分别为类叶升麻苷(Ⅰ-1)、异类叶升麻苷(Ⅰ-2)、crenatoside(Ⅰ-3)、cistanoside F(Ⅰ-4)、sinapoyl-4-O-β-D-glucoside(Ⅰ-5)、腺苷(Ⅰ-6)、β-谷甾醇(Ⅰ-7)、齐墩果酸(Ⅰ-8)、琥珀酸(Ⅰ-9)、咖啡酸(Ⅰ-10)、原儿茶醛(Ⅰ-11)、对羟甲基苯甲酸(Ⅰ-12)、β-胡萝卜苷(Ⅰ-13)、D-半乳糖醇(Ⅰ-14)和甘露醇(Ⅰ-15除化合物Ⅰ-1、Ⅰ-2和Ⅰ-3外，其余化合物均为首次从该植物中分离得到。2)通过大孔树脂富集该药材有效部位苯乙醇总苷，并采用HPLC测得其所含特征性成分类叶升麻苷的含量可达80%以上。3DPPH自由基清除试验显示紫花列当提取物及其所含的苯乙醇苷类化合物均具有较好的抗氧化能力，其清除DPPH自由基能力接近于抗坏血酸。4采用卡介苗整体致敏、脂多糖离体攻击构建大鼠原代肝细胞免疫性损伤模型，在体外观察类叶升麻苷的保肝作用。

The experimental results indicated that T_c~0,T_c~p,T_c~f and T_c~were increased,specially T_c~p increased by 15-20℃;and that the spherocrystal size,crystallization peak width,△T_c~L,D and△T_c~2were decreased.

实验表明，添加DMDBS使PP的T_c~o、T_c~P、T_c~f和T_c~均增大，尤其使T_c~P提高15-20℃，球晶尺寸显著缩小近10倍，而△T_c~L、D和△T_c~2减小且结晶峰宽变窄，表明结晶速率增大且晶粒分布均一细化。

Diethylthiophosphoryl chloride was synthesized by reacting phosphorus thiochloride with anhydrous alcohol in the presence of NaOH, and then a novel phosphorus-nitrogen- and sulfur-containing flame retardant, 1, 4-di(O,O-diethyl thiophosphoryl imine) benzene, was synthesized via the reaction of diethylthiophosphoryl chloride with p-phenylenediamine.

以三氯硫磷与无水乙醇在NaOH存在下合成了二乙基硫代磷酰氯，然后再与对苯二胺反应合成了新型的含磷、氮、硫的膨胀型阻燃剂：1，4-二（O，O-二乙基硫代磷酰亚胺基）苯。

By use of site mutation strategy and PCR technology, we obtained the gene P12X3C that includes full length PI, 2A, 3C and a part of 2B and 3B and the gene P12X3C3D that includes full length PI, 2A, 3C, 3D and a part of 2B and 3B. After being digested by restriction enzyme respectively, the gene P12X3C and the gene P12X3C3D were cloned into the pcDNA3.1 and pTARGET expression vector that were digested by the same enzyme. Recombinant plasmids were checked by restriction enzyme analysis and nucleic acid sequencing. Further more, recombinant plasmids were transfected into BHK-21 cells by using lipoid. The proteins of foot-and-mouth disease virus, which were expressed in BHK-21 cells, were confirmed by sandwich-ELlSA and fluoroscopy, and the capsid of FMDV was tested by electron microscope. In order to evaluate enhanced immune response of guinea pigs against FMDV, DNA vaccines which were designed to produce viral capsids lacking infectious viral nucleic acid and contained the gene P12X3C and the gene P 12X3C3D were injected respectively with FMDV 3D protein which was expressed in Pichia Pastoris Secreted expression System and purified or with pcDNA3.1/lFN which includes the gene IFN-a of cattle. Subsequently, Recombinant plasmids were injected to catties with or without pcDNA3.1/IFN. Anti-FMDV antibodies were detected by ELISA, and the T lymphocyte proliferation response was tested by MTT assay, neutralization antibodies liters were analyzed by micro-neutralization assay.

为研制带有O型口蹄疫病毒(Foot-and-Mouth Disease Virus，FMDV)China99株结构蛋白基因及多个非结构蛋白基因的DNA疫曲，本研究通过定点突变方法和PCR扩增方法，获得包含有FMDV China99株结构蛋白P1、非结构蛋白2A、3C以及部分2B、3B编码基因的片段P12X3C和包含有FMDV China99株结构蛋白P1、非结构蛋白2A、3C、3D以及部分2B、3B编码基因的片段P12X3C3D，将获得的基因片段直接／酶切后与同样处理的真核表达质粒连接，分别得到重组质粒pcDNA3.1／P12X3C和pcDNA3.1／P12X3C3D、pTARGET／P12X3C3D；对重组质粒进行序列测定、分析，并将重组质粒分别转染BHK-21细胞，通过双抗体夹心ELISA方法和间接免疫荧光标记方法检测细胞中FMDV抗原的表达，用电子显微镜观察病毒空衣壳的组装；为评价重组质粒作为DNA疫苗对实验动物及本动物的免疫效果，将重组质粒经肌肉注射方法接种豚鼠，并与酵母表达的纯化FMDV China99株3D蛋白及带有牛α干扰素的真核表达质粒pcDNA3.1／IFN分别／同时免疫，第二次免疫后第三周豚鼠攻以100ID_(50)或1000ID_(50)的O型FMDV China99株：随后将质粒pcDNA3.1／P12X3C、pcDNA3.1／P12X3C3D与带有牛α干扰素的真核表达质粒pcDNA3.1／IFN同时免疫牛，三周后经牛舌皮攻以10~4ID_(50)的O型FMDV China99株。

The split between the two groups can hardly be papered over.

这两个团体间的分歧难以掩饰。

This approach not only encourages a greater number of responses, but minimizes the likelihood of stale groupthink.

这种做法不仅鼓励了更多的反应，而且减少跟风的可能性。