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Firstly, we optimize the protocols of hippocampal primary culture and calcium phosphate transfection; and try to replace 430 nm laser with 458 nm laser for monitoring the FRET ratio of Cameleon.

首先，优化了细胞培养、磷酸钙转染方法，使用458 nm 激光代替430 nm 激光来进行荧光共振能量转移探测。

In terms of performance, Lorinser will outfit the E 500 with special camshafts, an upgraded exhaust system and revised engine software so it will produce 320 kW (435 HP) and 570 Nm of torque instead of 285 kW (388 HP) and 530 Nm.

在性能方面，劳伦士将装备的E 500特别凸轮轴，排气系统的升级和修改引擎软件，以便将产生320千瓦（ 435 HP ）和570 Nm的扭矩不是285千瓦（ 388马力）和530 Nm 。

The results indicate that all the sub-microparticles present sphere structures with diameters between 100 nm and 800 nm.

结果表明，所制得的亚微米粒粒径在100-800 nm之间，呈现球形结构。

The product has green-yellow fluorescence at excitation wavelength of 355 nm and emission wavelength of 478 nm, and the fluorescence intensity could be enhanced in the presence of acetone.

三、流动注射在线氧化荧光法测定氯普噻吨的的研究利用在硫酸介质中二氧化铅可使无天然荧光的氯普噻吨氧化而转变为在最大激发波长和最大发射波长分别为393nm和452nm的强荧光物质，而且丙酮对该荧光体系有强烈增敏作用的现象，建立了以醋酸纤维素固定化的二氧化铅作为固相反应器的流动注射在线氧化荧光法测定氯普噻吨的新方法。

Flow injection fluorometry for the determination of carbamazepine in pharmaceuticals and plasmaA simple, sensitive and specific spectrofluorimetric method has been developed for the determination of CBZ. The method was based on lead dioxide solid-phase reactor flow injection on-line oxidizing CBZ into fluorescent product in medium of phosphoric acid. The product has green-yellow fluorescence at excitation wavelength of 355 nm and emission wavelength of 478 nm, and the fluorescence intensity could be enhanced in the presence of acetone. The reaction conditions and experimental conditions were studied and optimized.

二、流动注射在线氧化荧光法测定卡马西平的研究利用卡马西平自身无荧光，经过氧化后可转变为在最大激发波长为355nm，最大发射波长为478nm的强荧光物质，而且丙酮对该荧光体系有增敏作用的现象，应用流动注射体系建立了以醋酸纤维素固定化的二氧化铅作为固相反应器、在磷酸介质中在线氧化荧光法测定卡马西平的新方法。

Methods Hyodeoxycholic acid and bilirubin in Calculus Bovis were separately identified by TLC, and the cholic acid content was determined by TLCS with the scan wavelength of λ=380 nm,λ=650 nm.

①用薄层色谱法分别鉴别猪去氧胆酸和胆红素；②用薄层扫描法测定胆酸含量，扫描波长：λ=380nm，λ=650nm。结果薄层色谱法可以区分体外培育牛黄和人工牛黄。

Excitation intensity of the complex is strong enough at 395 nm and the complex can be well excited by the 395 nm emission light of InGaN chip.

在395 nm处具有很强的激发强度，该配合物能够被395 nm发射的InGaN芯片发出的近紫外光激发而发红光。

Monitored at 614 nm, the strongest excitation wavelength located at 395 nm, which matched to the emission light of InGaN chip.

对614 nm 红光进行监控，其激发光谱在395 nm处具有最大的激发强度，与InGaN芯片发射的近紫外光激发相匹配。

According to the features of Brillouin scattering in different channel, the absorption spectra of molecule iodine absorption cell ranging from 470 nm to 550 nm are emulated in this paper.

依据不同信道中布里渊散射的特性，仿真出碘分子吸收池在470～550 nm区间的吸收光谱，在此基础上确定激光雷达系统激光中心频率的选取和鉴频器的设计方法。

For example, if the working wavelength range is reduced from 300~1100 nm to 400~800 nm, the single star position errors will be 0.0422"1.9652" and 3.3891 when the field view are (0°, 0°),(0°, 2°) and (0°, 4°) correspondingly, which are about 54%65%and 70%of the original errors. And the spot intensities are about 58%, 62%, 63% and 51% of the original when color temperatures of star are 9600 K, 7600 K, 5600 K and 3600 K correspondingly

例如，当响应波长从300~1100nm减小为400~800nm时，星敏感器视场(0°，0°)、(0°，2°)和(0°，4°)处的单颗恒星定位误差分别减小为0.0422&、1.9652&和3.3891，约为原来的54%、65%和70%；色温为9600K、7600K、5600K和3600K的恒星像斑能量分别约为原来的58%、62%、63%和51%。

Hanna: That's over now, isn't it?

都结束了，对吗

You must be ill. You look so pale.

你一定是病了，你的脸色苍白。