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Results:The results show that the survival rate of mammary epithelial cells were decreasing gradually with the increasing of the time of TGF-β1 treatment, and the survival rates of 24 h and 48 h groups were significantly lower than that of control group (p.05); the LDH activity of 6 h、12 h、24 h and 48 h groups were significantly higher than that of control group (p.05); the DNA degradation arisesed in 12 h, 24 h, and 48 h groups' mammary epithelial cells; the activity of Caspase-3 reach to a peak at 24 h, which was significantly higher than that of control group (p.05); the acinus of epithelial cells appears to break off and collapse with the increasing of treatment time, but the leydig structure keeps integrate;...

结果表明：1、5、10 ng/mL的TGF-β1均能抑制乳腺上皮细胞的增殖，且随浓度增加抑制作用加大，呈现剂量依赖性，其中10 ng/mL TGF-β1组与对照组差异显著（P.05）；10 ng/mL TGF-β1作用于乳腺上皮细胞24 h，随作用时间延长细胞凋亡率逐渐增加，6 h、12 h和24 h组与对照组相比差异极显著（P.01）；LDH活力也不断升高，6 h、12 h和24 h组与对照组差异显著（P.05）；DNA随 TGF-β1作用时间的延长发生不同程度降解；TGF-β1作用2 h~6 h细胞大量表达HSP70，6 h达到高峰，而后下降，2~12 h的表达量均显著高于对照组（P.05）。结论：TGF-β1可以抑制奶牛乳腺上皮细胞的增殖，具有浓度依赖性。10 ng/mLTGF-β1。。。

One day I passed a k I ndergarten, k I ds happened to be s I ng I ng and clapp I ng hands, and I stayed there for the whole morn I ng.

我就愿意听掌声，有一天我路过一个幼儿园，小朋友正在练习拍手歌，我在那儿呆了一上午。

Thi s paper s u mmari zed t he pri nci ples and met hods of t her mal techni que and its appli cati on i n st udyi ng ste m sap flo w ,s uch as water trans portati on pattern of ste m,i nternal and external factors affecti ng and regulati ng t he sap flo w ,calculati ng t he canopy sto matal conductance based on t he sap flo w value ,scali ng up i n s pace—ti me ,and sap flo w on ni ghtti me .

介绍了各种热技术测定树干边材液流的基本原理和方法，并对热技术在树干水分运输格局、影响和调节树干边材液流的内外因素、基于树干液流测定值推算林分冠层导度和尺度放大以及夜间树干液流等方面研究中的具体应用进行了综述。

The CPI showed negative correlation with the fatty acids contributed by vegetable wax.

二元酸以草酸（diC2）、丙二酸（diC3）为主，浓度年均值分别为548.13 ng·m-3、530.1 ng·m-3；二元酸总浓度呈明显季节变化特征，夏季最高（1539.36 ng·m-3），冬季最低（908.12 ng·m-3）。4个季节碳优先指数无显著差别，分别是15.3、12.4、11.5和13.4；CPI值与植物蜡贡献脂肪酸值呈现出一定的负相关。

It als o put for ward a seri es of meas ures and strategi es ,s uch as furt her wi dened funds rai si ng channel ,pro moted t he engi neeri ng constructi on rapi d developi ng rapi dly ,refor med t he plan manage ment mode ,enhanced t he i nvest ment standard ,set up t he i dea to protect t he for est ,coordi nated t he relati ons hi p bet ween t hi s project and ot hers ,co mbi ned wit h constructi ng har moni ous s oci ety and ne w s oci ali st countrysi de constructi on ,and coordi nated t he develop ment of sci enti c progress and project constructi on and s o on .

分析了陕西省三北防护林体系工程建设面临的问题，提出了为确保陕西省三北防护林四期工程顺利完成，应明确新时期工程建设的战略思想与建设任务；进一步拓宽筹资渠道，促进工程建设的迅速发展；改革计划管理方式，提高投资标准；树立防护林经营理念；协调好三北防护林工程与其他生态工程的关系；与构建和谐社会、社会主义新农村建设紧密结合；科技进步与工程建设协调发展等对策与措施。

The mc2155 carrying pMind-glmU-AS was induced by different concentration of tetracycline, and GlmU protein was detected in the mc2155 carrying pMind-glmU-AS by Western blotting. The results showed that tetracycline of 20ng/ml has obviously inhibited the growth rate of mc2155 carrying pMind-glmU-AS, but the amount of GlmU protein did not change compared to that of uninduced mc2155 carrying pMind-glmU-AS.

glmU反义RNA的诱导表达与GlmU蛋白的检测分别用5 ng/ml、10 ng/ml和20 ng/ml的四环素诱导携带pMind-glmU-AS表达载体的mc~2155菌株表达反义RNA，绘制细菌生长曲线，结果表明20 ng/ml浓度的四环素可抑制mc~2155/pMind-glmU-AS的生长，但Western Blotting分析结果显示经四环素诱导和未诱导的mc~2155/pMind-glmU-AS中，GlmU蛋白表达量无明显的变化。

Concentrations of HCH ranged from non-detectable to 19.63?ng·g~(-1) with an average of 0.50?ng·g~(-1); and coencentrations of DDT ranged from non-detectable to 176.01?ng·g~(-1) with an average of 4.42?ng·g~(-1), respectively. The concentrations of other Organochlorinated pesticides such as heptachlor, aldrin, and dieldrin were not detected.

采用气相色谱分析方法测定了官厅水库周边地区土壤样品中HCH、DDT、七氯、艾氏剂等多种有机氯农药的含量，采用数理统计方法研究了不同土地利用类型、不同采样方位与土壤中有机氯农药含量的关系。

It was concluded t hat furt her researches of ste m sap flo w usi ng t her mal techni ques could be enhanced ,i ncludi ng real water move ment and transfor mati on pattern i n ste m assi stant wit h ot her means ,cooperati on and co mpensati on functi on on t he i nternal and external factors affecti ng t he sap flo w ,as well as t he scali ng up i n tree water cons u mpti on based on t he sap flo w value by t her mal techni que .

目前，在运用各种热技术对林木树干液流的研究中，树干水分运输格局还需借助其他手段和方法进一步准确测定和佐证；影响树木边材液流内外因素的协同和补偿作用还需系统研究；基于热技术测定的树干液流值进行耗水时空尺度的扩大将成为该领域的研究热点之一。

METHODS: The osteoblasts were harvested from the frontal bone of 15-day SPF chicken embryos by the enzyme digestion, and treated with various mass concentrations of estrogen (0, 5, 10, 20, 100, 200, 400, 800, 2 000, 20 000 ng/L) and letrozole (0, 5, 10, 25, 50, 100, 250, 500, 1 000, 5 000 μg /L).

取15日龄SPF鸡胚，酶消化法获取鸡胚额骨成骨细胞，分别采用不同质量浓度雌激素(0，5，10，20，100，200，400，800，2 000，20 000 ng/L)和来曲唑(0，5，10，25，50，100，250，500，1 000，5 000 μg /L)处理。

Mr Ken Ng M.Sc. Environmental Pollution Control, B. Eng

Ken Ng 先生（环境污染防治理科硕士，工程学士

Do you know, i need you to come back

你知道吗，我需要你回来

Yang yinshu、Wang xiangsheng、Li decang,The first discovery of haemaphysalis conicinna.

1〕 杨银书，王祥生，李德昌。安徽省首次发现嗜群血蜱。