查询词典 NAA

Factors that influence percentage of transformation including time of preculture, dipping and coculture, type and content of antibiotic, day of delayed selection culture, intension of selection were studied. The effective transformation system of "Starkrimson" was developed as follow: Leaves precultured in darkness for three days were dipped into agrobacterium suspension for three minutes, then were cocultured in darkness on MS medium with TDZ0.5mg/L and NAA 0.3mg/L for three days, and then cultured in darkness on MS medium with TDZ0.5mg/L NAA0.3mg/L and Cef 250mg/L.

通过对叶片预培养时间、侵染时间、共培养时间、抑菌素种类和浓度、延时筛选时间、不同选择压等遗传转化影响因素的研究，建立的'新红星'苹果遗传转化体系为：叶片黑暗预培养3天，经农杆菌侵染3分钟，在含TDZ0.5mg/L，NAA0.3mg/L的MS培养基上黑暗共培养3天，然后加250mg/L Cef黑暗维持培养3天，再进行Km12.5mg/L的选择压黑暗培养至抗性愈伤出现；转为光照条件1-2次的继代筛选，获得抗性芽后，进行扩繁。

The study results indicated that the proliferation and radication of in vitro seedling were made simultaneously, the common-used culture medium was H+NAA 0.15 mg/L and radication rate was 80%~98%.

研究结果表明，组培苗增殖与生根同步进行，通用最佳培养基为：H+NAA0.15mg/L，生根率80%～98%，年增殖次数8次，繁殖系数为5。蛭石粉+泥炭土 1∶1 是三倍体毛白杨组培苗炼苗的最佳基质。

These uncontaminated explants showed that morphogenetic potential were 70%-75% and each responding explant had 1.2-1.4 shoots formation on MS medium supplemented with 2 mg/L NAA and 16 mg/L BA. Moreover, adding antibiotics (100 mg/L Rifampicin and 100 mg/L Gentamycin) to solidify MS contained 3% galactose were tested, results showed the lowest contamination rates were 20% and 1.0-1.2 shoots were regenerated per responding explant. The antibiotic treatments with 100 mg/L Rifampicin and 100 mg/L Gentamycin could reduce internal contaminants, while the addition of antibiotics into medium in vitro culture led to phototoxic phenomena of axillary explants, morphogenetic potential lost and the growth of regenerant was also inhibited.

以100 mg/L Rifampicin+100 mg/L Gentamycin或100 mg/L Rifampicin+100 mg/L Gentamycin+150 mg/L Streptomycin组合浓度处理者，具有显著降低污染率的效果分别为60%及50%；克服内生菌污染而建立初代无菌培养之腋芽培植体，则以100 mg/L Rifampicin或以100 mg/L Rifampicin+100 mg/L Gentamycin处理者，芽体再生率最高70%-75%，惟平均每一腋芽培植体芽体增殖数仅1.2-1.4个；另在醣种类及浓度与抗生素试验，显示100 mg/L Rifampicin+100 mg/L Gentamycin之抗生素浓度组合添加至含3g/L半乳糖的培养基，显著降低污染率至20%；但未污染之培植体，形态发生潜能低至40%以内，可反应生长之培植体芽体形成数也仅达1-1.2个之间，再生芽体之生长也显现抑制现象。

Non-DAI group also showed decreased NAA/Cr at splenium and increased Cho/Cr at genu of corpus callosum compared with control group (P 0.01), but the change degree was less than DAI group.

与对照组相比，非弥漫性轴索损伤组出现胼胝体膝部胆碱复合物/肌酸和磷酸肌酸升高和压部N-乙酰天冬氨酸/肌酸和磷酸肌酸降低(P 0.01)，但变化程度比弥漫性轴索损伤组低。

The cuttings were treated with auxin IBA, IAA and NAA of different concentration with rapid soaking 5—10 seco...

结果表明，6月初用5000ppm浓度IBA处理的插穗生根率达80％，这种方法可用于生产。

The ratio of monthly multiplications was 3-6, the vitrification phenomena of clump shoots was seldom happened, and efficiency seedlings were easily rooted besease of the optimal length between two bud burls. 3. Clump shoots subculture: On the ground of the basic media: amended MS+ BA0.2mg L-1+NAA0.1 mg L-1+saccharose3% 11 experiment was tested:(1)add to AC 0.01%, 0.03%, 0.06%, 0.1% respectively, at the same time, improving the concentration of BA to 0.5mg L-1 and NAA to 0.2 mg L-1 (2) add to VH 1mg L-1, 2 mg L-1 3 mg L-1 respectively;(3) amended MS (organic compoung and Fe2+ 1.5 double), add to saccharose 3%, 4% 5% respectively.

壮苗和有效芽诱导：以改良MS+BA0.2mg·L~(-1)+NAA0.1mg·L~(-1)+3％蔗糖+0.55％卡拉胶为基础和对照，①分别加入0.01％、0.03％、0.06％、0.1％的活性炭，同时提高BA的浓度为0.5-1mg·L~(-1)，IAA浓为0.1-0.5mg·L~(-1)；②分别加入1mg·L~(-1)、2mg·L~(-1)、5mg·L~(-1)的生物素，③改良MS(铁盐、有机物1.5倍)，蔗糖分别增加为4％和5％，共11个处理，结果表明：活性炭对邓恩桉组培苗的生长起抑制作用，生物素所起的作用不明显，邓恩桉组培继代苗的生长离不开细胞分裂素和生长素，一旦缺少继代苗很快枯萎死亡。

The height, stem of seedling, total length of roots; leaf area, chlorophyll content of plats which was sprayed with ABT 50 mg/L reached 27.53%, 61.54%, 70.91%, 64.23% and 21.43% respectively, which were higher than that of the control. NAA and ABT had obvious effects on accelerating o the seedling growth of Castanopsis fissa.

结果］2种生长调节剂对大叶栎苗木的生长有明显的促进作用，尤以喷施ABT 50mg/L的处理表现最佳，其苗高、地径、根系总长、叶面积、叶绿素含量分别比对照提高了27.53%、61.54%、70.91%、64.23%、21.43%。

Different callus were obtained by culturing the leave explants of one double flower Petunia plant on the media supplemented with different combinations of auxin and cytokinnins.

方法］以重瓣矮牵牛叶片为外植体，使用不同浓度BA与NAA的组合在黑暗条件下诱导愈伤组织。

Among three auxins , IAA at low concentration was the best for growth of Type Ⅰ hairy root, while 1mg/l IBA was the most suitable for growth of Type Ⅱ hairy root.

在发状根的继代培养基中添加外源生长激素（IAA、IBA、NAA），低浓度的IAA更适合于Ⅰ型发状根的增殖培养，而在促进Ⅱ型发状根的增殖和侧根形成方面，1mg/lIBA的作用最强。

The explants cultured on MS' mediums with addition of NAA and 6-BA and irradiated under lights with different wavebands.

光质作为一种物理因子已广泛用于植物组织培养中光形态建成及其各种反应机理的研究m'叫们。

I didn't watch TV last night, because it .

昨晚我没有看电视，因为电视机坏了。

Since this year, in a lot of villages of Beijing, TV of elevator liquid crystal was removed.

今年以来，在北京的很多小区里，电梯液晶电视被撤了下来。