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Muscovy相关的网络例句

查询词典 Muscovy

与 Muscovy 相关的网络例句 [注:此内容来源于网络,仅供参考]

Uh, I'll have the lobster bisque and muscovy duck, please.

我要龙虾贝肉汤和美洲家鸭

These results showed that the muscovy duck reovirus can cause immunosuppreasion of Muscovy duck.

上述结果提示,番鸭呼肠孤病毒能导致番鸭免疫抑制。

Waterfowl parvovirus infection is caused by goose parvovirus and Muscovy duck parvovirus and might lead to severe economic losses.

中文摘要水禽小病毒感染症由Goose parvovirus与Muscovy duck parvovirus所引起,此二病毒经常造成养禽业重大的经济损失。

A pair of primers for amplification of σC gene was designed and synthesized according to the published Muscovy duck reovirus S4 gene sequence. The specific RT-PCR product was success-fully obtained and cloned from the Muscovy duck reovirus ZJ99 strain.

参考已发表的番鸭呼肠孤病毒的S4序列设计了1对扩增σC蛋白基因的引物,对mDRV ZJ99株的RNA抽提物进行RT-PCR扩增,获得了特异性的扩增片段。

The Ultrastructure changes of liver, heart, lung, kidney, spleen, bursa and thymus of Muscovy ducks infected experimentally with muscovy duck reovirus were observed.

对人工感染番鸭呼肠孤病毒发病雏番鸭的心、肝、肺、肾、脾脏、胸腺、法氏囊等7种实质器官的超微结构进行了观察。

In order to diagnose disease caused by muscovy duck parvovirus rapidly and accurately, and at same time, further study the taxonomic relationship between muscovy duck parivovirus and goose parvovirus, two pairs of PCR primers (LHMP1/LHMP2 and LHGP1/LHGP2) were designed respectively base on complete nucleotide sequences of Muscovy duck and Goose parvoviruses registered in the GenBank, The LHMP1 for the upper one and the LHMP2 for the lower one, corresponding to the specific nucleotides sequence 454-472 and 1173-1155 respectively in MDPV, and the LHGP1 for the the upper one and the LHGP2 for the lower one, corresponding to the specific nucleotides sequence 454-472 and 1173-1155 respectively in GPV.

为了能快速、准确地对番鸭细小病毒病进行诊断,同时为了能进一步弄清番鸭细小病毒与鹅细小病毒在分类学上是一种怎样的关系,比较GenBank中MDPV和GPV的基因序列,针对这两种病毒左阅读框编码非结构蛋白基因序列的相同区段,分别构建两对PCR引物。第一对引物LHMP1/LHMP2是根据MDPV的基因序列设计的,上游引物针对MDPV基因序列的454~472;下游引物针对基因序列的1173~1155,为19个碱基,扩增跨幅为719bp。

An attenuated vaccine strain of Muscovy Duck Reovirus, which was suitable for propagating in chicken embryo fibroblast, was obtained by serial passage alternately between Muscovy duck embryo fibroblast and CEF.

应用番鸭胚成纤维细胞和鸡胚成纤维细胞交替传代选育出适应CEF繁殖的番鸭呼肠孤病毒弱毒疫苗株。

The Muscovy Company of London issues the first equity shares.

伦敦的Muscovy公司发行第一张股票。

Muscovy duckling parvovirusis is a new infectious disease of Muscovy duckling with 22~62% disease rate and 85% fatality rate.

雏番鸭细小病毒病是一种新的番鸭传染病,其发病率为22~62%,致死率为85%。

The minor core protein s C-encoding gene of Muscovy duck reovirus was cloned into theprokaryotic expression vector pET32a. The recombinant plasmid pET32a-s C was amplified andextracted after being transformed into E.coli DH5a competent cells. Restriction analysis withEcoRⅠand SacⅠand sequences analysis indicated that the recombinant plasmid was inserted withcorrect open reading frame. The fusion protein about 50 ku was produced after induction with 0.15mmol/L IPTG of E.coli competent cells transformed with pET32a-sC. The SDS-PAGE andWestern-bloting test indicated that the fusion protein reacted with the convalescents sera of duckinfected with Muscovy duck reovirus. The indirect ELISA method was developed by using thepurified fusion s C protein as coating antigen. The optimal concentration of s C was 5μg/ml, thedilution of serum sample was 1:40; The results showed that preparation of an ELISA by using sCas coating antigen in detecting 50 field duck sera in comparison with the AGIP were more sensitiveand specific than agar gel immuno-diffusion AGIP test. The results suggest that presence ofantibody against viral protein sC in duck may be a good indicator by the sC-ELISA for detectionof duck infection with reovirus.

同时,本研究将编码外壳蛋白σC的基因克隆于原核表达载体pET32a上,经过EcoRⅠ和SacⅠ双酶切鉴定和序列分析后,得到阳性重组质粒pET32a-σC;将阳性重组质粒pET32a-σC转化到大肠杆菌BL-21感受态细胞中进行诱导表达,经SDS-PAGE和Western-blbtting检测分析,融合表达的蛋白能够与番鸭呼肠孤病毒感染的康复鸭血清发生特异性反应;将融合表达的蛋白纯化后作为包被抗原,建立了检测鸭血清中呼肠孤病毒抗体的间接酶联免疫吸附试验检测方法,此方法中抗原的最佳包被浓度为5μg/ml、标准阳性血清的最适稀释倍数为1:40倍,用此方法对50份鸭血清样品进行检测,并与琼脂糖凝胶扩散试验检测抗体的法相比较,证明此ELISA方法具有良好的特异性和敏感性,本研究为今后鸭呼肠孤病毒诊断试剂盒的研制奠定了基础。

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