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The 1/2 MS medium supplemented with 1.0 mg·L-1 BA and 0.1 mg·L-1 NAA was very beneficial to the protocorm differentiation and propagation of D.candidum .The highest protocorm propagation index was obtained from the medium containing the activated carbon.The highest root numbers and length were observed in plants growing in 1/2 MS medium containing 0.5 mg·L-1 NAA.
结果与结论:添加20%马铃薯液有利于种胚的萌发,种胚萌发率为79.37%;BA 1.0 mg.L-1和NAA 0.1 mg.L-1最有利于原球茎增殖,培养基中添加2%活性碳原球茎增殖倍数高;NAA有助于试管苗的生根,不同含量NAA以0.5 mg.L-1时试管苗平均根数最多和平均根长最长。
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These uncontaminated explants showed that morphogenetic potential were 70%-75% and each responding explant had 1.2-1.4 shoots formation on MS medium supplemented with 2 mg/L NAA and 16 mg/L BA. Moreover, adding antibiotics (100 mg/L Rifampicin and 100 mg/L Gentamycin) to solidify MS contained 3% galactose were tested, results showed the lowest contamination rates were 20% and 1.0-1.2 shoots were regenerated per responding explant. The antibiotic treatments with 100 mg/L Rifampicin and 100 mg/L Gentamycin could reduce internal contaminants, while the addition of antibiotics into medium in vitro culture led to phototoxic phenomena of axillary explants, morphogenetic potential lost and the growth of regenerant was also inhibited.
以100 mg/L Rifampicin+100 mg/L Gentamycin或100 mg/L Rifampicin+100 mg/L Gentamycin+150 mg/L Streptomycin组合浓度处理者,具有显著降低污染率的效果分别为60%及50%;克服内生菌污染而建立初代无菌培养之腋芽培植体,则以100 mg/L Rifampicin或以100 mg/L Rifampicin+100 mg/L Gentamycin处理者,芽体再生率最高70%-75%,惟平均每一腋芽培植体芽体增殖数仅1.2-1.4个;另在醣种类及浓度与抗生素试验,显示100 mg/L Rifampicin+100 mg/L Gentamycin之抗生素浓度组合添加至含3g/L半乳糖的培养基,显著降低污染率至20%;但未污染之培植体,形态发生潜能低至40%以内,可反应生长之培植体芽体形成数也仅达1-1.2个之间,再生芽体之生长也显现抑制现象。
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Adopt optimized transformation system of the melon cultivar: the explant infected in the engineering bacterium the OD560 of which was 0.30 for 15min, and co-cultured for 3days; Choosing cultivated in MS +1mg/L 6 - BA + 500mg/L carb + 75mg/L Kan, extend cultivated in MS +0.05mg/L 6 - BA + 300mg/L carb + 75mg/L Kan and rhizogenic cultivated in 1/2MS + 200mg/L cef + 25mg/L Kan.
对甜瓜栽培品种的转化采用已优化好的转化体系:农杆菌菌液OD560=0.3,侵染时间为15min,外植体选用近轴端子叶;共培养时间为72h。
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The results showed that the MS2 fragmentation pathways of DASSSB were affected by the character and position of substitutes on salicylidene phenyl. The C=N cleaving to form fragmentation ion [M+H-283](superscript +) was dominant for DASSSB compounds with electron-donating group on the C4 of the salicylidene phenyl; while fragmentation ion [M+H-254] (superscript +) forming by C-C cleavage of the β-position of C=N was dominant for DASSSB compounds with electron-withdrawing group on the C5 of the salicylidene phenyl.
结果显示,这些化合物的ESI-MS/MS裂解方式与它们分子中水杨醛苯环上取代基的性质和位置有关:当水杨醛苯环上4位连接供电子取代基时,分子以CN双键断裂为主,生成[M+H-283]碎片离子;当水杨醛苯环上5位连接吸电子取代基时,分子以CN双键β位的C-C键发生断裂为主,生成[M+H-254]碎片离子。
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A method for determination of the four metabolites of nitrofuran antibiotics such as 3-amino-2-oxazolidinone, 3-amino-5-morpholinomethyl-2- oxazolidinone, semicarbazide and 1-aminohydantoin in Poultry Tis- sues by LC-MS-MS was developed.
本文建立了用高效液相色谱-串联质谱测定禽肉组织中3-氨基-2-唑烷基酮、5-甲基吗啉-3-氨基-2-唑烷基酮、氨基脲和1-氨基-2-内酰脲4种硝基呋喃类抗生素代谢物的方法。
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Benchmarking the novel scorer on a large set of experimental MS/MS spectra, it is demonstrated that PepSearch performs significantly better than the widely used software SEQUEST.
对大容量的串联质谱数据集的测试表明,根据算法开发的软件 PepSearch 比目前最常用的软件 SEQUEST 有更好的鉴定准确性。 PepSearch 可从 http://compbio.sibsnet.org/projects/pepsearch 下载。
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Benchmarking the novel scorer on a large set of experimental MS/MS spectra, it was demonstrated that PepSearch performs significantly better than the widely used software SEQUEST.
经过用37044个串联质谱搜索包含88374个蛋白序列的数据库进行多肽鉴定的测试表明,根据本算法开发的软件PepSearch比目前最常用的软件SEQUEST有更好的鉴定准确性。
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During the interview between Ms. Mei Ng and Ms. Lizette Smook, Lizette mentioned that the working experience in a heavily polluted textile factory inspired the idea of environmental protection in her.
在吴方笑薇女士与Lizette Smook女士的访谈过程中,Lizette提到,她在高污染纺织工厂的工作使她第一次萌发了环保理念。
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The average operation time of DSP is 2 ms with one primary window size 230×200, 0.4 ms with three secondary Windows size 60×60 respectively. Under the tanglesome background, prompt tracking the targets of low contrast is achieved.
1个 3 2 0× 160主窗口的 DSP平均处理时间为 2 m s,3个 60× 60副窗口的 DSP平均处理时间各为 0 。4ms,实现了复杂背景下低对比度目标的快速跟踪。
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Using optimized linear assemble language to improve real timing and precision. The average operation time of DSP is 2 ms with one primary window size 230×200, 0.4 ms with three secondary Windows size 60×60 respectively. Under the tanglesome background, prompt tracking the targets of low contrast is achieved.
为了提高实时性和精确度,采用线性汇编优化实现。1个 3 2 0× 160主窗口的 DSP平均处理时间为 2 m s,3个 60× 60副窗口的 DSP平均处理时间各为 0 。4ms,实现了复杂背景下低对比度目标的快速跟踪。
- 相关中文对照歌词
- Me & Ms Pain
- No Lastimes Más
- Qué Más Da
- Un Juego Más
- Dibujando Un Corazón (Más Allá)
- Nada Más Quien Piensas
- Este Muerto No Llevo Más
- Ms
- Ms. Bird
- Dale Más Duro
- 推荐网络例句
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Singer Leona Lewis and former Led Zeppelin guitarist Jimmy Page emerged as the bus transformed into a grass-covered carnival float, and the pair combined for a rendition of "Whole Lotta Love".
歌手leona刘易斯和前率领的飞艇的吉他手吉米页出现巴士转化为基层所涵盖的嘉年华花车,和一双合并为一移交&整个lotta爱&。
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This is Kate, and that's Erin.
这是凯特,那个是爱朗。
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Articulate the aims, objectives and key aspects of a strategic business plan.
明确的宗旨,目标和重点战略业务计划。