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Studies on callus induction, adventitious shoot regeneration of Cirsium japonicum DC. var. takaoense Kitamura using leaf and stem-node as explants were conducted with different strength of MS medium, sucrose concentration and growth regulator combination in this investigation.
中文摘要本研究以白花小蓟无菌苗之叶片与茎节培植体为材料,进行不同MS培养基浓度、蔗糖浓度、NAA配合BA或TDZ浓度组合对愈合组织形成及不定芽诱导之影响。
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The results showed :The most suitable medium for buds induction from cotyledon of Citrullus lanatus was MS���6-BA 3.0mg/L+NAA 0.1mg/L.
研究结果表明:子叶芽诱导的最佳培养基配方为MS+6-BA3.0mg/L+NAA0.1mg/L。
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Plantlet regeneration from cotyledon of Citrullus lanatus cv. Zhangkang No. 4 was studied. The results showed that aseptic seedlings should be cultured in dark for 3d, then exposed to a photoperiod of 16/8h/d for 3d. The callus induction rate was higher in cotyledons placed facing downwards to the medium than in those facing upwards. The highest induction rate occurred in MS medium containing 6-BA (2.0mg/L), kinetin (1.0mg/L)and GA3 (1.0mg/L). The induction initiated in dark and took 7 days, while callus growth and differentiation proceeding for 7 days under 16/8h light-dark cycles. A highest rate of embryogenic callus was obtained after 3 successive subcultures.
以无子西瓜郑抗4号无菌苗子叶为外植体,进行了体细胞胚发生及植株再生的研究,结果表明,无菌苗应先进行3d暗培养,然后采取光照16h/d和黑暗8h/d培养3d;将子叶的叶面朝下放置于培养基上的愈伤组织诱导率高于叶面朝上的培养方式;子叶诱导胚性愈伤组织的最适培养基配方为MS+6-BA2.0mg/L+KT1.0mg/L+GA31.0mg/L;诱导需要在黑暗条件下启动,进行7d暗培养,而生长分化于光照16h/d和黑暗8h/d条件下培养7d;继代3次得到的胚性愈伤组织最多;最适生根培养基配方为1/2MS+IBA0.3mg/L。
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A simple, rapid and accurate method for the determination of hydroxyproline in collagenous proteins by high performance liquid chromatography-electrospray mass spectrometry (HPLC-ESI/MS) was developed.
建立了一种简单、快速地测定胶原蛋白中羟脯氨酸的高效液相色谱-电喷雾质谱(HPLC-ESI/MS)方法。
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When the embryogenic calli were transferred to medium MS+5mg/L ABA+50g/L PEG+5mg/L AgNO〓 for one month, a lot of normal cotyledonary somatic embryos were produced.
当胚性愈伤组织转到MS+5mg/LABA+50g/L PEG+5mg/L AgNO〓的分化培养基上,1个月后可产生大量正常的子叶期成熟体细胞胚。
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It was shown that 40 ppm MS-222 addition at 25℃ can efficient increase the crucian survive time.
结果表明:在25℃时选用质量分数为4×10^(-5)kg/mg的MS-222可有效延长鲫鱼的无水保活时间。
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The essential oil of the foliage from four Cupressus chenegiana populations distributed in Minjiang and Daduhe rivers was analyzed by GC-MS.
使用GC-MS仪对大渡河和岷江流域的四个不同居群的岷江柏叶精油进行了化学组分研究。
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Methods: A cyclohexyl group introduced and a series title compounds synthesized All of them were confirmed by MS, 1H-NMR, et al. The antifungal activities were also evaluated against the eight common pathogenic fungi.
引入环己基侧链结构,合成一系列目标化合物,所有化合物结构均经MS、1H-NMR等谱确证;选择8种真菌为实验菌株,测定其体外抗真菌活性。
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The results indicated the best medium for supension co-culture with Daucus carota and Daucus carota is MS, 2, 4-D 2 mg/L, NAA 1 mg/L and KT 0.2 mg/L, after 25 d the polysaccharide content of co-culture reaches the highest level 0.203 mg/g, and the saponin content of co-culture is 1.85mg/g, higher 3.35% than saponin of Panax ginseng culture.
结果表明,人参与胡萝卜悬浮混合培养的最佳基质为MS、2,4-D2mg/L、NAA1mg/L、KT0.2mg/L,在培养25d时混合培养物的多糖含量达到最高,浓度达到0.203mg/g,混合培养物的皂苷含量为1.85mg/g,比人参悬浮培养物皂苷含量高出3.35%。
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The ethanol extract was dissolved with HCl, the acidic extract was basified to pH9-10 with NH_3-water and extracted with CHCl_3 to give total alkaloids. five alkaloids were isolated with column chromatography and preparative thin-layer chromatography, and three of them were identified as dehydrocorydaline、 corynoline and dihydrosanguinarine on the basis of spectral analysis MS,~1H-NMR,~(13C-NMR and physicochemical properties. All of them were found for the first time from this plant.
为研究灰绿黄堇的化学成分,本文将灰绿黄堇乙醇提取物采取酸提取、碱游离后萃取的方法得到总生物碱,然后利用柱色谱、制备薄层色谱等方法分离、纯化了灰绿黄堇中5种生物碱,并利用IR、MS、~1H-NMR、~(13)C-NMR等方法鉴定了其中的3种,分别为去氢紫堇碱、紫堇灵、二氢血根碱,均为首次在此植物中发现。
- 相关中文对照歌词
- Me & Ms Pain
- No Lastimes Más
- Qué Más Da
- Un Juego Más
- Dibujando Un Corazón (Más Allá)
- Nada Más Quien Piensas
- Este Muerto No Llevo Más
- Ms
- Ms. Bird
- Dale Más Duro
- 推荐网络例句
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We have no common name for a mime of Sophron or Xenarchus and a Socratic Conversation; and we should still be without one even if the imitation in the two instances were in trimeters or elegiacs or some other kind of verse--though it is the way with people to tack on 'poet' to the name of a metre, and talk of elegiac-poets and epic-poets, thinking that they call them poets not by reason of the imitative nature of their work, but indiscriminately by reason of the metre they write in.
索夫农 、森那库斯和苏格拉底式的对话采用的模仿没有一个公共的名称;三音步诗、挽歌体或其他类型的诗的模仿也没有——人们把&诗人&这一名词和格律名称结合到一起,称之为挽歌体诗人或者史诗诗人,他们被称为诗人,似乎只是因为遵守格律写作,而非他们作品的模仿本质。
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The relationship between communicative competence and grammar teaching should be that of the ends and the means.
交际能力和语法的关系应该是目标与途径的关系。
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This is not paper type of business,it's people business,with such huge money involved.
这不是纸上谈兵式的交易,这是人与人的业务,而且涉及金额巨大。