查询词典 Moi

Effects of multiplicity of infection , time of infection and the nutrient post infection on production were studied.

二 研究感染指数MOI、感染时间和感染后培养液营养对昆虫细胞培养产物表达的影响。

Nous sommes quatre dans notre famille : mon p re, ma m re, ma sur et moi.

我们有四口人，我爸，我妈，我姐姐和我。

The model of HCC was established by injecting subcutaneously the cells in different groups in nude mice.

结果 重组腺病毒AdEasy-GFP-ASmrp滴度可达2.5×10〓efu／ml，MOI为100时，即可致90％以上的肝癌细胞SMMC-7721／ADM被感染。

Adenovirus 5第二军医大学博士学位论文英文摘要胸心外科专业(WAd5) and PDC-MLC-VEGF were transferred to the adenoviral packaging cell HEK 293cell by lipofectamine 2000 mediated gene transfer method to pack the virus; The desired Advectors were purified by density gradient ultracentrifuge and titrated in 293 cells afteridentified.

Ad.mlc-hVEGF165 体外转染心肌细胞的实验研究分离培养乳鼠心肌细胞，在同一 MOI 值下，Ad.mlc-hVEGF165 和 Ad.hVEGF165分别转染心肌细胞，以 RT-PCR 法和 Western 印迹分析法分别检测转染后心肌细胞内VEGF mRNA 的转录及 VEGF 蛋白的表达，以 ELISA 法检测转染后心肌细胞分泌的VEGF 蛋白质，MTT 等方法观察其对促内皮细胞增殖作用。

Cell proliferation and viability were assayed 48h after transfection, and MDA-7 demonstrated selective inhibition of tumor cell growth, inhibitory rates of A549, Hela and HepG2 cell lines were 25%, 20% and 19%, respectively, but had no significant effect on human fetal kidney derived 293 cell line. Hela cell was screened by G418 for 2 weeks after pcDNA3-MDA-7 and monolayer colony was counted, its monolayer colony formation was 30% of cells transfected with pcDNA3. 0 vector. CMV-driven MDA-7 adenovirus vector was constructed. 293 showed no significant apoptosis during adenovirus packaging and the unpurified adenovirus titer was about 1×10〓pfu/ml. Cos 7, A549, Hela, HepG2 and Hep3B cell was infected with Ad-GFP at different MOI.

二。黑色素细胞分化相关蛋白-7（MDA-7）的克隆及功能研究：利用RT-PCR方法从5月龄人胚胎脾细胞扩增MDA-7的编码序列，经测序鉴定序列与文献报道一致后，与真核表达载体pcDNA3.0连接，构建pcDNA3-MDA-7表达载体，瞬时转染293、A549、Hela和HepG2细胞后抽提细胞总RNA，RT-PCR结果显示表达载体可介导MDA-7在不同细胞系中有效表达；转染48h后测定细胞增殖和活力，MDA-7可选择性抑制肿瘤细胞的增殖，对A549、Hela和HepG2细胞的抑制率分别为25％、20％和19％，但是对人胚肾来源的293细胞生长无明显影响。pcDNA3-MDA-7载体转染Hela细胞后，以G418筛选2周后计数单层细胞集落形成数，计数仅为转染pcDNA3.0空载体的细胞的30％左右。

"Not so fast, Your Holeyness," said Fred, and darting past the gaggle of middle-aged witches heading for the procession, he said, Here – permetiez moi to assister

&嘿，别急，小心呐，&弗雷德说着，径直穿过一群中年女巫，朝他的目标冲了过去，这里——能否允许我为两位小姐效劳？

Then someone from the audience screamed,"Voulez vous coucher avec moi?"

然后有人从观众大叫，voulezvouscoucher舒曼语言？

F: ensemble non, mais moi je peux imaginer faire aE: not all, but I can imagine doing it

大概是回答禁片问题的：不会，但我可以设想一下那么做

Brian : excusez - moi , numero two

我们第一，别人第二

Current ly, Sims is re sponsible for handling Moi selle's

慎昌物流很荣幸可为慕丝在本港之业务提供服务。

Singer Leona Lewis and former Led Zeppelin guitarist Jimmy Page emerged as the bus transformed into a grass-covered carnival float, and the pair combined for a rendition of "Whole Lotta Love".

歌手leona刘易斯和前率领的飞艇的吉他手吉米页出现巴士转化为基层所涵盖的嘉年华花车，和一双合并为一移交&整个lotta爱&。

This is Kate, and that's Erin.

这是凯特，那个是爱朗。