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After superfusing the brain slice with guanylate cyclase inhibitor,MB(3 pmol/L) for 30 min,SDR of 10 units showed significant increase as compared with control. However,MB failed to abolish the effect of L-arg on hippocampal neurons.

3给脑片灌流鸟昔酸环化酶抑制剂亚甲基蓝(3umol/L 30 min后，10个单位的平均放电频率较对照时明显增加，但亚甲基蓝不能消除L-arg对海马神经元的作用。

Superimposing rotating magnetic field could reduce the total oxygen concentration of molten steel, in the situation of 80~120 r/min, the removal efficiency of total oxygen could be reached to 70% for molten carbon steel and 38% for molten stainless steel.

旋转磁场的施加使钢液中全氧含量迅速降低，在80~120r/min的转速下，碳钢可以获得约70％、不锈钢可以获得38％以上的全氧含量去除效率。

Methods The derivation with dansyl chloride was followed by normal-phase chromatography with a mobile phase of acetone/chloroform (2:98) at a flow rate of 1 mL/min and with the detection wavelength set at 425nm.

唾液经纯化、丹酰氯衍生化后用苯提取，以Hypersil BDS CN为正相色谱柱，丙酮－氯仿(2：98)为流动相，流速1 mL/min进行测定，检测波长为425 nm。

Ingersoll-Rand rotary screw air compressor provides tjs drill rig with 22.5m3/min compressed air ('1.4Mpa).

配套的英格索兰空气压缩机，为钻机提供1.4Mpa、22.5m3/min的压缩空气。

Firstly, headspace solid-phase microextraction was established to determine AITC by orthogonal design. Extraction temperature, stir speed, extraction time, and desorption time were set at 40℃, 900r/min, 30min, and 5min, respectively. Under these optimal conditions, volatile flavor compounds in mustard oil, wasabi, and 'Chongcai'leaf mustard product were identified by SPME/GC/MS. In these three samples, 6,12,17 constituents were identified respectively; Allyl isothiocyanate was the main flavor compound, which accounted for 73.45%, 64.25%, 32.74% of total volatile compounds, respectively. Carbon disulfide, a hydrolysis product of AITC, accounted for 14.37% 25.40%, 51.67% of total volatile compounds respectively. There were some other isothiocycannates, such as butane, 1-isothiocyanato-, isobutyl isothiocyanate, 1-benzene, 4-isothiocyanato-, benzene (2-isothiocyanatoethyl)-, etc.

首先采用正交实验设计优化固相微萃取技术的前处理条件，结果表明，以萃取温度40℃、搅拌速度900r／min、萃取时间30min、解吸时间5min为最佳条件；在此最优条件基础上，利用SPME／GC／MS联用技术分析芥末油、青芥辣、冲菜的挥发性风味成分，分别检出6、12、17种化合物，主要风味物质均为AITC，相对丰度分别为73.45％、64.25％、32.74％；AITC的水解产物二硫化碳的相对丰度分别为14.37％、25.40％、51.67％；还检测到了其它几种异硫氰酸酯，如异硫氰酸丁酯、异硫氰酸异丁酯、异硫氰酸丁烯酯、2-苯乙基异硫氰酸酯等。

It comes up to 97% when the aluminum sulphate dose is 10 mg, coagulation time 10 min and pH 5.5-6.0

硫酸铝质量10 mg，絮凝时间10 min，pH在5.5~6.0范围内，去除率可达97%左右

The mobile phase was 0.1 mol/L ammonium oxalate + N,N-dimethylformamide + 0.2 mol/L diammonium phosphate (75+15+10, by vol) adjusted to pH 8 by aqueous ammonia. Column temperature was 30℃, flow rate was 0.8 ml/min, and detection wavelength was 365 nm.

使用C18反相柱和二极管阵列检测器，以0.1mol/L草酸铵溶液+二甲基甲酰胺+0.2mol/L磷酸氢二铵溶液=75+15+10(用氨水调pH值至8.0左右)为流动相，柱温30℃，流速0.8ml/min，检测波长365nm，外标法对水合霉素的有效成分进行定量分析。

The advance speed through the base is the10～15mm/min.

穿越时的推进速度控制在10～15mm/min。

The biogenic amines in test samples were extracted by 10% trichloroacetic acid and then derived with benzoyl chloride. The HPLC analysis for the samples were conducted as the following condition: mobile phase was a gradient elution program of methanol and water, the flow rate was 0.8 mL/min.

用10%的三氯乙酸提取样品中的生物胺，采用苯甲酰氯衍生处理后，以甲醇/水为流动相，进行梯度洗脱，流速0.8 mL/min，紫外检测器波长为254 nm。

Seven biogenic amines were extracted from a sample with 6% perchloric acid, then derivatized with benzoyl chloride. 0.02 mol/L borate buffer (pH 9.2) containing 0.06 mol/L sodium deoxycholate-methanol （95∶5, v/v）was used as a running buffer. The detection wavelength was set at 214 nm. The separation was achieved within 12 min. Good linearities were observed from the calibration plots in the concentration ranges.

样品经6%过氯酸萃取后，由苯甲酰氯衍生化，以含0.06 mol/L脱氧胆酸钠的0.02 mol/L硼酸(pH 9.2)-甲醇(体积比为95∶5)混合液为电泳介质，电泳电压25 kV，温度25 ℃，检测波长214 nm，在12 min内实现了7种生物胺的完全分离。7种生物胺的浓度与其峰面积在一定的范围呈良好的线性关系，检出限除组胺为15 μg/g外，其余均为5 μg/g。

I didn't watch TV last night, because it .

昨晚我没有看电视，因为电视机坏了。

Since this year, in a lot of villages of Beijing, TV of elevator liquid crystal was removed.

今年以来，在北京的很多小区里，电梯液晶电视被撤了下来。