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This paper studied the conditions of protoplast preparation of Oenococcus oeni,including the concentration of preculture and lysozyme,lytic time,hypha culture time,etc.As a result,hypha culture time 20 h,penicillinG Na 0.5 μg/mL,lysozyme 1 mg/mL,lytic time 30 min,SMM as osmotic pressure stabilizer,ATB culture medium of regeneration culture mediu...

结果表明，酒酒球菌SD-2a原生质体制备的最佳条件为：菌龄20 h，青霉素G钠质量浓度0.5μg/mL，酶质量浓度1mg/mL，酶解时间30 min，渗透压稳定剂采用SMM缓冲液，再生培养基采用添加0.7 mol/LKCl的ATB培养基，再生培养时采用双层培养基厌氧培养，可以保证较好的形成率和再生率。

The friable embryogenic callus with small grains and without proembryo formation was screened in the process of continuous subcultures for 4~6 times on the medium with low content of sucrose from embryogenic cultures induced from immature embryos in litchi (Litchi chinensis Soon.). The well-scattered embryogenic cell suspensions could be rapidly established from the friable embryogenic callus in 2 liquid initial MS media respectively supplemented with 2,4-D 2 mg/L or 2,4-D 2 mg/L, KT1 mg/L and AgNO3 5 mg/L shaking at 100~120 r/min within 10-14 days.

荔枝幼胚诱导的胚性培养物在低糖条件下连续继代4~6次左右，可筛选到颗粒细小、不含原胚的松散型胚性愈伤组织；以这种松散的胚性愈伤组织作为起始材料，在附加2，4-D 2mg/L或2，4-D 2mg/L、KT1 mg/L、AgNO3 5mg/L的MS液体启动培养基上振荡培养（100~120 r/min）10~14 d，即可建立起分散性良好的胚性悬浮细胞系。

The S. pullorum on the surface of shell eggs was completely killed and E. coli O157:H7 logarithm of counts decreased to less than 1.0 log10 by NEW containing 12 mg/L available chlorine for 3 min without viable cells in the washing water after treatment.

当中性电解水清洗消毒被人工污染的鸡蛋表面时，有效氯质量浓度为12 mg/L、处理3 min可将鸡蛋表面的鸡白痢沙门氏菌全部杀灭，大肠杆菌O157：H7菌落数对数降低到1.0 log10 以下，且处理废液中没有残存菌，无二次污染问题。

The as synthesized samples were characterized by XRD. The results show that a single phase compound Gd2Zr2O7 with a cubic pyrochlore structure is successfully synthesized at 5.2 GPa pressure and 1 873 K temperature with 30 min holding time.

通过XRD对合成样品进行了结构表征，结果证实，在5.2 GPa和1 873 K条件下，保温保压30 min，成功地合成出单一物相的、具有立方烧绿石结构的Gd2Zr2O7化合物。

DK77 HE Series electric spark digital controlling machine tool High Efficiency and fine quality Multi-technical breaking-through, six paten honored Low price, high quality All-closed loop control, automatic multi-cutting, automatic machinery precision error compensating and highly intellectual Highest precision≤0.008mm Highest smooth finish rate, Ra≤0.58um and reedless Great benefits: at Ra≤1.2um, the process speed could obtain 75mm2/min Highly integrated: clear circuit, strong stability, reliable and easy to fix Unique wire winding system employed, smooth running, noiseless and long useful life Industry controlled computer system, PLC logical control and functional module design materialize the low error rate

DK77□□HE系列高性能电火花数控线切割机床惊人的效率、优良的品质多项技术性的突破、集六项专利于一身慢走丝的效果、快走丝的价格全闭环控制、自动多次切割、自动补偿机械精度误差、智能化程度高最高精度≤0.008mm 最高光洁度Ra≤0.58um，表面无条纹高效益：在Ra≤1.2um时，加工速度75mm2/min 高集成化：电路清晰、稳定性高、安全可靠、维修方便采用独特的运丝系统，运行平稳、无噪声、寿命长采用工业控制计算机系统、PLC逻辑控制、功能模块化设计，使产品故障率大大降低。

80% ethyl alocohol dissolvent and 2.0 ml/min repercolation speed are optimal repercolation conditions, normal pressure aqueous bath condense preparing casting material with the method of thick mastic is fundamentally feasible, the extractive technology of dependable effective component is prepared for advanced development of semen cassiae drop pill.

以80%乙醇为溶剂和2 ml/min渗漉速度为最适宜的重渗漉条件，常压水浴浓缩制备投料用稠膏的方法基本可行，为研制决明子滴丸提供可靠的有效成分提取工艺。

The residua were air-blown in a batch reactor at an air flow of 3 L.kg-1.min-1, 260 and 280℃ for 0.5-11.5 h respectively.

在3 L.kg-1.min-1空气通入量，260和280℃下对所得渣油在实验室釜式氧化装置进行了0.5-11.5 h空气氧化。

By setting the retting duration of 20 hours, the factors affecting the residual gum percentage of the retted kenaf fibers are studied. The experimental results show clearly that the following factors have the significant influence on the residual gum percentage of the retted kenaf fibers and show the obvious variation tendency. The preferred processing parameters are as follows: pH value 7. 0、bath ratio 1: 50、reuse percentage of retting liquid 50%、air flow volume 2. 5L/min、temperature 40℃、nitric additives (ammonia is better than nitrates) percentage 0.45%.By comparison with anaerobic retting, the retting duration using aerobes is much shorter than that using anaerobes.

确定了以好氧微生物脱胶作为本课题的脱胶路线后，在特定的脱胶时间（20小时）条件下，通过分析不同参数条件下的残胶率，研究了好氧微生物脱胶的各种影响因素对红麻脱胶的影响规律，并优选出最佳脱胶液回用比为50％，最佳pH值为7.0，最佳气流量为2.5L/min，最佳浴比为1：50，最佳水浴温度为40℃，最佳含氮添加剂为铵盐类化合物，最佳含氮添加剂用量为0.45％。

When the cooling rate is 1001000℃/min, some primary Si are granular, others are rodlike.

当冷却速度为1001000℃/min时，初晶硅呈颗粒状和棒状的混合态。

When the cooling rate is 3570℃/min, the aspect ratio of rodlike Si increases.

当冷却速度达到3570℃/min时，初晶硅均呈现长棒状。

In the negative and interrogative forms, of course, this is identical to the non-emphatic forms.

。但是，在否定句或疑问句里，这种带有"do"的方法表达的效果却没有什么强调的意思。

Go down on one's knees;kneel down

屈膝跪下。。。下跪祈祷