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The pellet was resuspended in ddH2O (10 g pellet l–1) and autoclaved for 30 min at 121 °C. The elicitor preparation was stored at –20 °C until used.

球被溶于蒸( 10克弹丸l - 1 )和蒸30分钟，在121 ° C的诱导准备被储存在-20℃直至使用。

Water with gradient elution,the velocity of flow was 1.0 ml/min,detection wavelength was at 205 nm.

水为流动相，采用梯度洗脱，流速为1.0 ml/min，检测波长205 nm。

The gradient elution of methanol in ammonium acetate buffer allowed elution of all the amines in less than 30 min.

梯度洗脱甲醇在铵醋酸缓冲区允许洗脱所有的胺在更少超过30分钟。

S Grace of the Empyrean Lord recharge decreased from 5 to 3 min.

主神的恩宠CD时间由5分下调到3分。

Cleric□s Grace of the Empyrean Lord recharge decreased from 5 to 3 min.

主神的恩宠CD时间由5分下调到3分。

ResultsThe optimal extraction conditions were as follows:temperature at 50℃, pressure at 35 MPa,velocity of flow 4.5 ml·min-1,100％ ethanol as entrainer.

最佳萃取工艺参数为：萃取压力35 Mpa，萃取温度50℃，乙醇浓度100%，夹带剂流速4.5 ml·min-1，萃取率达到0.425%。

The results showed that the significance of the effects of these factors on the oil extraction efficiency decreaseed in the order:extraction pressure, time, temperature and entrainer dosage, and the maximum extraction efficiency of watermelon seed oil could reach 52.18% under the extraction pressure of 40 MPa, temperature of 45℃, time of 150 min and the ethanol dosage of 7%.

结果表明，影响西瓜籽油萃取率的因素主次顺序为：萃取压力＞萃取时间＞萃取温度＞夹带剂用量；最佳提取条件为萃取压力40 MPa，萃取温度45℃，萃取时间150 min，夹带剂乙醇用量7%。在最佳条件下西瓜籽油萃取率为52.18%。

Results enunciate that the best time is 150 min, the best amount is 6g/L Under the normal temperature.

结果表明：在常温下的最佳吸附时间为150min，最佳投放量为6g/L。

When pH is 5, temperature is 80℃, stir speed is 200 r/min, the dosage of sodium oleate is between 0.4 and 0.5 times of the quantity of ferrous, the effect of enwrap is best and the size of magnetic nanoparticles is small, the magnetism of magnetic nanoparticles is strong.

当PH=5，温度为80℃，搅拌速度为200r/min，油酸钠的用量大约为Fe(上标 2+)的0.4~0.5倍时，包裹效果最好，得到的磁性颗粒小、磁性强。

Result Based on directly regenerated plant of in vitro plant of Xinjiang cotton, the cotton epicotyl treated by pre-culture for 3-5 d was immersed in bacteria for 15 min and co-cultured for 2 d. Cephalothin at 100 mg/L was used to inhibit bacteria after 50 mg/L kan selection. And the combination of cytokinin with relatively high content and auxin with low content was used in its directly budding culture to obtain resistant seedlings.

结果］在新疆棉花离体培养植株直接再生植株基础上，经3~5d预培养处理过的棉花上胚轴，在浸菌15 min共培养2 d后，经50 mg/L kan筛选，用1000 mg/L头孢霉素抑菌，并用相对高含量细胞分裂素和低含量生长素的组合对其进行直接出芽培养，从而获得抗性小苗。

You can do some assertiveness training.

你可以进行一些自信训练。

We were well on the path to making a rear-wheel-drive global platform," says Mays."

我们正致力于建立一个后轮驱动的平台，"Mays这样说道。"