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Successful separation of Cd-pheophytin a, Cd-pheophytin b, chlorophyll a, chlorophyll b, pheophytin a and pheophytin b in 14min was firstly accomplished by reversed-phase HPLC at a flow rate of 0.8ml/min, with the mobile phase of methanol: acetone (80: 20, v/v).

首次以甲醇：丙酮＝80：20为流动相，流速为0.8ml/min，在C〓柱（5μm，4.0×250mm）上，14min内成功实现了镉叶绿素a和镉叶绿素b配合物与叶绿素a，b和脱镁叶绿素a，b的高效液相色谱分离。

The cotyledon and seed coat were cooked at 120℃ 10~50 min and compare their content of pH and chlorophyll. The result showed pH value of seed coat（6.25~5,46）was lower then cotyledon（6.35~6.06）. The increase of acidity and heat treatment caused content of chlorophyll in the seed coat to decrease（6.57~1.28 mg/100 g）and form pheophytin.

绿豆子叶、种皮於120 ℃烹煮10~50 min时，发现种皮pH值（6.25~5.46）较子叶（6.35~6.06）为低，於酸性环境与热造成种皮中叶绿素含量降低（6.57~1.28 mg/100g），并形成褐黄色的脱镁叶绿素，此现象与种皮b值增加（21.55~34.85）具关联性。

The ultrathin cryosections and ethanolic phospho tungstic acid staining sections of rat atrial cardiocytes were prepared. Quantit ative electron probe Xray microanalysis of Hall continuum method was adopted to measure the alteration of Ca content in ASG at the exact time intervals of 15, 30, 60, 120 min after the adrenaline administration.

冷冻超薄切片技术和磷钨酸-乙醇特殊染色技术制备大鼠心房切片。X射线显微定量分析采用Hall连续谱法，用以测量在肾上腺素处理后15、30、60和120 min时ASG中钙含量的变化。

The pial arteriolar dilator response to hypercapniawas significantly attenuated in the clonidine group (14%±4%, 4%± 4%, 6%± 6%, and 5%± 7% for beforeand 30, 60, and 90 min, respectively).

可乐定组软膜小动脉对高碳酸血症的扩张反应明显减轻（给药前及后30， 60， and 90 min 时分别为14%±4%，4%±4%，6%±6%和5%±7%）。

The liquid absorption value and growth of the bacillus was measured after 24, 48 and 72 hour. It was showed that the most effective level of maltose in the medium is 5%. The media with pimelic acid was centrifugated 30min at 10000r/min, room temperature

本研究还将添加5%的麦芽糖的生物素培养基分组，分别加入0、0.2、0.4、0.6、0.8、1.0、1.2、1.4、1.6、1.8、2.0g/L的庚二酸，接种，在37℃，220r/min条件下进行振荡培养，然后测定24、48和72h时菌液的吸收值、pH值、生物素和庚二酸含量，确定庚二酸添加量为1.0g/L时，培养液中的生物素产量最大，庚二酸利用率最高。

Rats in the model control group were fed with ethanol and pinguid food by intragastric gavage and were put on the static treadmill of 0 slope, with the frequency of 30 min/d.

模型对照组：给予乙醇＋高脂饮食灌胃，放进静止的跑台不运动，0坡度，30 min/d。

By orthogonal experimental design method, the optimized degumming parameters have been confirmed: preacid processing, the concentration of sulfuric acid 2 g/L, treatment time 120 min, treatment temperature 60℃, bath ratio 1:15; alkali treatment, the alkaline concentration of one-pot 7.5 g/L, that of two-pot 9 g/L, treatment time of one-pot 3.5h, that of two-pot 2h, treatment temperature 100℃, bath ratio 1:20, each concentration of the Sodium Silicate, carbamide, and JFC 2%.

预酸处理，硫酸溶液质量浓度2 g/L，处理时间120 min，处理温度60℃，浴比1：15；碱处理，一煮碱溶液质量浓度7.5 g/L，二煮碱溶液质量浓度9 g/L，一煮处理时间3.5 h，二煮处理时间2 h，处理温度100℃，浴比1：20，其中一煮与二煮中添加2%的硅酸钠、尿素和JFC。

The setting of parameters is as follows; sample weight of 0.15g, the maximum work current of 350mA, the flow capacity of carrying gas of 3L/min, the purity of oxygen of 99.99%, the precleaning time of l0s, the predelay time of 10s, the analytical period of 60s, the comparison level of 1.0%. The blank of ceramic crucibles could be reduced to minimum by burning them for 2h at 1100℃.

其最佳测定条件为：样品称量为0.15g左右；最大工作电流350mA，载气流速为3L/min，载气纯度99.99%，陶瓷坩埚需经1100℃灼烧2h后，可使空白降至最小，预清洗时间10s，预延时间10s，破元素最短分析时间为60s，比较水平为1.0%。

In the fermenter,the continuous cultivation was conducted with continuous feeding after the production process of bioflocculant was stabilized with preculture for 9 h at inoculum dosage of 5%. The bioflocculant yield was 2.392 g/L and the maximal flocculation rate reached 98.1% when the feed flow rate was 4 mL/min.

在发酵罐扩大连续培养中以5%的接种量接种后经过9 h预培养达到稳定生产絮凝剂后，以4 mL/min的补料流量进行连续培养，生产的絮凝剂产量达到2.392 g/L，且最大絮凝率为98.1%。

The results Showed that the highest transformation efficiency was obtained through the following transformation procedure: after preculture for 2 d, the explants were in fected 3 min with 50 mg/L acetosyringone and OD600=0.5 Agrobacterium culture liquid, and then co-cultivated for 2 d. Resistant shoots from leaf disks were screened on MS0.4 mg/L NAA + 100 mg/L Cef medium supplemented with 10 mg/L Km.

结果表明：2 d的预培养，OD600值约为0.5的农杆菌菌液添加50 mg/L AS，侵染时间3 min，2 d的共培养，从分化培养基获得的抗性芽在生根培养基MS+0.4 mg/L NAA+10 mg/L Km+100 mg/L Cef上进行延迟筛选，有利于获得较高的转化效率。

In the negative and interrogative forms, of course, this is identical to the non-emphatic forms.

。但是，在否定句或疑问句里，这种带有"do"的方法表达的效果却没有什么强调的意思。

Go down on one's knees;kneel down

屈膝跪下。。。下跪祈祷