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The greatest efficiency in Agrobacterium-mediated transformation of lettuce cotyledons(2～3 days-old) was obtained by 10-time dilution of Agrobacterium cells, 15-min inoculation time,1-day pre-culture,4-day co-culture and without AS using the MS(Murashige and Skoog\'s,1962) medium supplemented with 0.2 mg/L 6-benzylaminopurine,0.1 mg/Lα-naphthaleneacetic acid,100 mg/L Kan and 500 mg/L Carb,.

在农杆菌介导的皱叶生菜子叶(2～3 day苗龄)转化试验中，采用含BA 0.2mg/L，NAA 0.1 mg/L，Kan 100 mg/L，Carb 500 mg/L的MS培养基，最优的转化条件为农杆菌稀释倍数为10倍，预培养时间为1 day，共培养时间为4 day，浸染时间为15 min，不添加乙酰丁香酮。

The results show that the maximum growth quantity of Nocardia can be achieved after culturing 120 h and obvious selective adsorption of Nocardia on the surface of pyrite and sphalerite can be detected. The maximum adsorption rate on the surface of pyrite is 96.99%, whereas it is less than 20% in the case of sphalerite. Adsorption equilibrium can be attained within 20 min on the surfaces of the two minerals. pH value is the key factor influencing upon selective adsorption, and the difference of adsorption quantity on the surfaces of two minerals is apparent when pH is between 4 and 10. Slurry concentration over 6 g/L is favorable to selective adsorption. Nocardia cell concentration, stirring speed and temperature have no obviously effect on selective adsorption.

结果表明：培养120 h后诺卡氏菌达到最大生长量；诺卡氏菌在黄铁矿和闪锌矿表面发生明显的选择性吸附，在黄铁矿表面的最大吸附率可达到96.99％，而在闪锌矿表面的吸附率大都在20％以下；诺卡氏菌在两种矿物表面于20 min内即可达到吸附平衡； pH值是影响诺卡氏菌在两种矿物表面发生选择性吸附的关键因素， pH在4～10之间时，选择性吸附现象明显；矿浆浓度超过6 g/L时有利于发生选择性吸附；细胞悬浊液浓度、搅拌速度和温度对吸附效果影响不大，均可产生选择性吸附。

The microstructural nomograph of these Al-Si alloys is constructed to provide for the selection of optimum addition of Sr or Sb in production practice Experimental results indicate a threshold of Sr content in Al-Si alloy modification, over it, the structure of Al-Si alloy may be completely modified even at quite slow cooling rate down to 4.5℃/min.

实验结果表明，Al-Si合金加Sr后变质存在一个变质阈值，超过这个阈值时，即便在投缓慢的冷速(4.5℃/min)下，仍能获得完全变质的组织。这个变质阈值随着冷速的减慢而增大。

Its. OH scavenging activity was nonsensitive to high-temperature and sensitive to oxygen. But treatment of 75 ℃ for 30 min could highly decrease its sensitivity to oxygen.

其清除OH的能力耐高温，但对空气敏感，但75℃、30 min处理可以显著降低葡萄伤流液对空气的敏感度。

Nonsynchronous electrical cardioversion together with intravenous loading dose 150mg of amiodarone was administed,and it would be repeated once after 10min when the treatment had no effect,followed by 0.5～1.5mg/min infusion for 24～36 hours,and amidarone was given to patients orally.The curative effect and side effect were observed.

对急性心肌梗死突然发作持续性室速和室颤的9例患者，在非同步电转复的同时静脉注射胺碘酮150mg，无效者10min后再重复一次，继之以0.5～1.5mg/min静点维持24～36h，同时口服胺碘酮，观察其疗效和副作用。

The column of Hypersil-ODS C18:250mm×4.6mm 5μm; the mobile phase: 1.5% orthophosphoric acid and 10% methane alcohol;the detection wavelength: 276nm;velocity of flow: 1. 0 ml/min; temperature: 40℃;the content is measured in the way of external standard method of apex area.

方法］采用Hypersil-ODSC18(250mm×4.6mm5μm)色谱柱；流动相为甲醇—1.5%磷酸10%甲醇溶液(40：60，v/v)；检测波长为276nm；流速为1.0ml/min；柱温：40℃。

Orthostatic test (+75° 20 min)was conducted on each subject pre-and post-HDBR.

三组卧床前、后进行20 min +75°的立位实验。

The results show that the vertical sintering speed changes notably with the proceeding of sintering process. After ignition and thermal retardation the bed permeability deteriorates and the sintering speed decreases notably because of the progress of physical chemistry reaction, formation and broaden of combustion zone, and formation of overwet zone. In about 25 min, the over wet zone vanishes, so the bed permeability meliorates and the speed arises rapidly.

结果表明：垂直烧结速度并非固定不变，而是随着烧结过程的逐步进行发生显著变化；点火保温后，随烧结物理化学反应的进行，燃烧带逐渐形成并变宽且产生过湿现象，导致料层的透气性显著变差，烧结速度显著下降，直至约25 min后过湿层完全消失，透气性变好，烧结速度迅速上升。

Methods Rhizoma Gastrodiae, Pellis Ovis, and Calculus Bovis Sativus in Tianhuang Houzao powder were identified by TLC. The content of Gastrodin in the powder was determined by HPLC. The HPLC condition was as follows: column was Phenomenex C18 (4.60 mm×250 mm, 4 m), the mobile phase consisted of methanol-0.05% phosphoric acid (3:97), the UV detection wavelength was at 220 nm, the flow rate was 1.0 ml/min with column temperature at 25℃.

用薄层色语法鉴别天黄猴枣散中天麻、猴枣、体外培育牛黄；用高效液相色谱法对制剂中天麻素进行定量分析，Phenomenex C18 (4.60 mm×250 mm， 4 m)色谱柱，流动相为甲醇－0.05%磷酸溶液(3：97)；检测波长220 nm，流速1.0 ml/min，柱温25 ℃。

E. irradiation power is 200W, the ratio of phenylamine to benzoic acid is 1:1.5, the irradiation time is 5 min, and the reaction temperature is 180℃.

利用正交实验设计法对影响收率诸因素进行了考察，确定最优工艺条件为：在微波功率200W下苯胺与苯甲酸的摩尔比1：1.5、辐射时间5 min、反应温度180℃。

In the negative and interrogative forms, of course, this is identical to the non-emphatic forms.

。但是，在否定句或疑问句里，这种带有"do"的方法表达的效果却没有什么强调的意思。

Go down on one's knees;kneel down

屈膝跪下。。。下跪祈祷