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The chromatographic conditions were as followed: C8 column, 0.045 mol/L ammonium phosphate monobasic solution∶acetonitrile (2∶1)(adjusted to pH7.35 with triethylamine) as mobile phase, temperature: 40℃, the flow rate: 1.0 ml/min, detection wavelength: 210nm.

采用C8柱，以0.045mol/L磷酸二氢胺溶液∶乙腈(2∶1)(用三乙胺调pH至7.35)为流动相，流速1ml/min，检测波长210nm，柱温40℃。

Methods Using Diamonsil ODS1 C18 Column, acetonitrile- 0.033mol/L Potassium Phosphate Monobasic acid(35:65) as the mobile phase,detection wavelength as 265nm and flow rate was 1.0ml/min.

HPLC用外标一点法，Diamonsil ODS1 C18柱，乙腈- 0.033mol/L磷酸二氢钾溶液(35：65)为流动相，流速为1.0ml/min，λ=265nm。

The chromatographic conditions were as followed: C8 column, 0.045 mol/L ammonium phosphate monobasic solution∶acetonitrile (2∶1)(adjusted to pH7.35 with triethylamine) as mobile phase, temperature: 40℃, the flow rate: 1.0 ml/min, detection wavelength: 210nm.

采用C8柱，以0.045mol/L磷酸二氢胺溶液∶乙腈(2∶1)(用三乙胺调pH至7.35)为流动相，流速1ml/min，检测波长210nm，柱温40℃。结果阿奇霉素的线性范围为50.3～1006μg/ml，平均回收率100.1%，r=1.0000，RSD=0.8%(n=9)。

The chromatographic conditions were as followed: C8 column, 0.045 mol/L ammonium phosphate monobasic solution∶acetonitrile (2∶1)(adjusted to pH7.35 with triethylamine) as mobile phase, temperature: 40℃, the flow rate: 1.0 ml/min, detection wavelength: 210nm.

采用C8柱，以0.045mol/L磷酸二氢胺溶液∶乙腈(2∶1)(用三乙胺调pH至7.35)为流动相，流速1ml/min，检测波长210nm，柱温40℃。结果阿奇霉素的线范围为50.3～1006μg/ml，平均回收率100.1%，r=1.0000，RSD=0.8%(n=9)。

The HPLC condition consisted of C18 column(250 mm×4.6 mm,5 μm),mobile phase of 0.1 mol/L monopotassium phosphate-methanol(70 : 30,v/v) at the flow rate of 1.0 mL/min and at detection wavelength 280 nm.

本法采用C18色谱柱，流动相为0.1mol/L磷酸二氢钾溶液(用磷酸调节pH值至2.5)-甲醇的混合溶液(按v∶v=70∶30配制)，检测波长为280nm，流速为1.0mL/min。

Inosine -5'- monophosphate (5'-IMP) and guanosine-5'-monophosphate (5'-GMP) in samples were separated successfully using an Inertsil ODS-3 column (250 mm×4.6 mm, 5 μm) when 0.02 mol/L monopotassium phosphate (pH 4.5) was employed as the mobile phase with a flow rate of 1.0 ml/min at 250 nm and 25℃.

采用Inertsil ODS-3色谱柱(250 mm×4.6 mm， 5μm)；以0.02 moL/L磷酸二氢钾溶液(pH 4.5)为流动相，流速1.0 mL/min，柱温25℃，检测波长250 nm，同时分离了样品中5'-鸟苷酸二钠和5'-肌苷酸二钠。

Melamine in eggs was extracted and the protein in samples was precipitated with acetronitrile, melamine was separated on a Luna SCX column (Luna 5u SCX 250×4.6mm) with monopotassium phosphate(MKP 0.05mol/L, pH=3.00), acetronitrile buffer solution (70:30, by volume) as the mobile phase at flow rate 1.50mL/min and the detection wavelength was 240nm, quantitative with external standard method.

采用乙腈作为样品中蛋白质的沉淀剂和三聚氰胺提取剂，流动相为0.05mol/L(pH=3.00)的磷酸二氢钾乙腈缓冲溶液，检测波长为240nm，流速为1.50mL/min，SCX(Luna 5u SCX250×4.6mm)色谱柱进行分离，HPLC-UV检测，外标法定量。

Results show the optimal conditions for the moringa seed oil extraction are as follows: using petroleum ether as solvent,the ratio of solvent to moringa seeds is 8∶1,extraction time 40 min and extracting twice with the aid of 28 kHz ultrasonic.At the conditions,the ...

结果显示，超声波辅助溶剂萃取辣木籽油的最佳条件为：溶剂石油醚，提取次数2次，提取时间40 min，超声波频率28 kHz，液料比8∶1，在此条件下，辣木籽的平均出油率为35.85%，提取率96%，表明超声波辅助溶剂萃取法是一种可用于萃取辣木籽油的适宜方法。

Methods 100 cases were randomized into treatment group in which 50 cases were treated with Safflower injection via carotid artery infusion, added Safflower injection 20ml into 0.9% sodium chloride injection 60ml with the 4ml/min velocity, once a day; control group in which 50 cases were treated with venous drip of Safflower injection, added Safflower injection 20ml into 0.9% sodium chloride injection 500ml. After therapy, the curative effect、 myodynamia and hemorrheology were compared between the two groups.

将100例脑梗死患者随机分为红花注射液颈动脉灌注组50例和红花注射液静脉滴注组50例，治疗组：于0.9%氯化钠注射液60ml中加入红花注射液20ml，以4ml/min的滴速经颈动脉灌注；对照组：以红花注射液20ml加入0.9%氯化钠注射液500ml中静滴，20天后对两组疗效进行分析并观察治疗前后肢体肌力及血液流变学变化。

Objective To observe the clinical efficacy of treatment in acute cerebral infarction by Safflower injection via carotid artery infusion.Methods 100cases were randomized into treatment group in which50cases were treated with Safflower injection via carotid artery infusion,added Safflower injection20ml into0.9%sodium chloride injection60ml with the4ml/min velocity,once a day;control group in which50cases were treated with venous drip of Safflower injection,added Safflower injection20ml into0.9%sodium chloride injection500ml.After therapy,the curative effect、myodynamia and hemorrheology were compared between the two groups.

目的 探讨颈动脉灌注红花注射液治疗急性脑梗死的临床疗效方法将100例脑梗死患者随机分红花注射液颈动脉灌注组50例和红花注射液静脉滴注组50例，治疗组：于0.9%氯化钠注射液60ml中加入红花注射液20ml，以4ml/min的滴速经颈动脉灌注；对照组：以红花注射液20ml加入0.9%氯化钠注射液500ml中静滴，20天后对两组疗效进行分析并观察治疗后肢体肌力及血液流变学变化。

In the negative and interrogative forms, of course, this is identical to the non-emphatic forms.

。但是，在否定句或疑问句里，这种带有"do"的方法表达的效果却没有什么强调的意思。

Go down on one's knees;kneel down

屈膝跪下。。。下跪祈祷