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F. Nees and their metabolites by HPLC, the mobile phase was acetonitrile-water (0.1% methanoic acid) for gradient elution, Column temperature was 25℃, flow rate was 1.0mL/min, the detection wavelength was at 254nm..

采用HPLC对穿心莲及其代谢产物进行研究,以乙腈-0.1%甲酸水进行梯度洗脱,柱温为25℃,流速为1.0mL/min,检测波长为254nm。

Using UPLC system,WATERS ACQUITY UPLC BEH C~(18)(2.1 mm×50 mm,1.7μm) column and the mobile phase of 1% methanoic acid solution-acetonitrile with the flow rate of 0.6 mL·min~(-1),the determination wavele...

使用 UPLC 色谱系统,BEH C~(18)色谱柱(2.1mm×50mm,1.7μm);流动相为1%甲酸溶液—乙腈;流速为0.6mL·min~(-1);检测波长为280nm。

The experimental conditions were as follows: C18 chromatographic column, mobile phase of MeOH-H2O (both include 0.1% methanoic acid), flow rate at 1.0 mL/min, gradient elution, detection wavelength at 290 nm and room temperature.

采用C18色谱柱,甲醇-水(含0.1%的甲酸)梯度洗脱;流速为1.0mL/min,检测波长290nm;柱温为室温。

The optimal condition of the methanolysis transesterification was as follows: 50% enzyme based on oil weight;methanol/oil mole ratio 3∶1;shaking speed 150 r/min;added water content 5%;reacting at 50 ℃ for 12 h.

最佳工艺条件为:在正己烷的反应介质中,酶量50%,醇油摩尔比3∶1,反应温度50℃,转速150r/min,添加质量分数5%的水分,反应12 h后产物脂肪酸甲酯得率可达92.3%。

Results showed that AOT reversed micellar systems provided a suitable micro-environment for biodiesel production. The optimal parameters were: the ratio of water and AOT 11, AOT concentration 50 mmol/L, temperature 40℃, pH 7, molar ratio of methano and oil 3, and rotating speed 180 r/min. The transesterification rate reached 90% when a 3-step methanolysis protocol was used.

研究结果表明: AOT/异辛烷反胶束体系为Candida sp.99-125脂肪酶催化提供了较为合适的微环境,在W0为11,表面活性剂浓度为50 mmol/L,温度为40℃,缓冲液pH值为7的AOT/异辛烷反胶束体系中,醇油摩尔比为3∶1,摇床转速为180 r/min,采用12h3次流加1 mol当量的甲醇,单批最高酯转化率可以达到90%。

The Mannich-type addition of O , O'-dialky phosphite to 2-fluorobenzaldehyde and 4-trifluoro- methylaniline was carried out in one pot by means of adopting three kinds of green synthetic methods, microwave irradiation, ionic liquid and free solvent-catalyst condition. According to the results, the synthetic method was optimized without catalyst and solvent at 100 ℃ and 15 min, and it was economic, convenient, high yields, timesaving and environment friendly.

采用微波辐射﹑离子液体和无溶剂无催化剂三种绿色合成方法,分别进行了以亚磷酸酯、邻氟苯甲醛和对三氟甲基苯胺为原料的类Mannich一锅法合成反应研究,经合成筛选,优选出控制反应温度100 ℃、无溶剂和无催化剂加热反应15 min的合成方法,实验结果表明该方法经济、简便、产率高、反应时间短及对环境友好。

The separation was achieved within 4 min using a BEH Shield RP18 column (100 mm 2.1 mm, 1.7 μm) with 50 mmol/L monoammonium phosphate solution (pH 6.5) and acetonitrile as the mobile phase.

以BEH Shield RP18柱(100 mm 2.1 mm,1.7 μm)为分析柱,以50 mmol/L的磷酸二氢铵(pH 6.5)和乙腈为流动相进行梯度洗脱,3种磷酸腺苷在4 min内实现了较好的分离。

Methods: The HPLC with a C18 column (150mm×4.6mm, 5μm) was used. Methanol-0.05mol/L Potassium phosphate monobasic buffer solution-Acetic acid glacial (40:60:1) was used as mobile phase. The flow rate was 1.0mL/min, and the detection wavelength was at 333nm.

采用高效液相色谱法,用C18色谱柱(150mm×4.6mm, 5μm),以甲醇-0.05mol/L磷酸二氢钾溶液-冰乙酸(40:60:1)为流动相,流速为1.0mL/min,检测波长333nm。

The chromatography conditions were as follows: methanol - 0.1mol/L monobasic potassium phosphate (50∶50) as mobile phase, flow rate being 1.0ml/min and detecting wavelength was 290nm.

方法采用高效液相色谱法,色谱柱为Diamonsiltm C18柱;流动相:甲醇-0.1 mol/L磷酸二氢钾水溶液(50∶50);流速:1.0 ml/min;检测波长:290 nm。

MethodsHigh Performance Liquid Chromatography was performed Diamonsil C18. The chromatography conditions were as follows: methanol - 0.1mol/L monobasic potassium phosphate (50∶50) as mobile phase, flow rate being 1.0ml/min and detecting wavelength was 290nm.

方法采用高效液相色谱法,色谱柱为Diamonsiltm C18柱;流动相:甲醇-0.1 mol/L磷酸二氢钾水溶液(50∶50);流速:1.0 ml/min;检测波长:290 nm。

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