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The results showed that the Saccharopolyspora spinosa SP06081 protoplast yield was the highest under these conditions: the collected mycelium from SP06081 grown in Tryptic Soy Broth medium with 0.2% glycin for 48 h was treated by 0.1 mg/mL lysozyme at 28oC for 20 min, then plated on the R2YE medium with sucrose as osmotic stabilizer, the number of regeneration protoplast was up to 108/mL. The protoplast-regenerated strains exhibited changes in morphology and antibiotic production, 29.3% protoplast-regenerated strains was characterized by loose mycelium and abundant broken branches as did their parent. Among them, 58.2% strains presented the trend to positive variation in spinosad yield, with the highest spinosad yield of up to 582.0 mg/L, 85.6% higher than that of their parent.

结果表明：菌体在添加0.2%的甘氨酸的TSB培养基中培养48 h收集， 0.1 mg/mL 溶菌酶， 28oC作用20 min制备原生质体，将原生质体涂布于以蔗糖为渗透压稳定剂的R2YE培养基中，原生质体再生数目最多，达108个/mL以上；原生质体再生菌株在形态和抗生素产量上产生分化， 29.3%的再生菌株形态上保持与亲本菌株一致，具有菌丝松散，断裂分枝多的特点，其中53.2%的再生菌株多杀菌素产量变异向正方向移动，最高产量达到582.0 mg/L，比亲本菌株提高85.6%。

Results The best experimental conditions for the new method were as follows: hydrochloric acid concentration 5%; sodium borohydride solution, potassium bromate and potassium bromide solution, 2ml each; flow rate 0.6L/min.

结果］本法的最佳实验条件为盐酸溶液浓度5%，硼氢化钠溶液和澳化剂的用量各为2ml，载气流量0.6L/min。

A feasible synthesis process is pH value=9 and precipitant titration rate=5 ml/min.

可行的工艺为：溶液的pH=9、沉淀剂滴入速度为5ml/min。

The female and male pronucleus formed and fused about 45～50 min after insemination.

授精后约45～50 min雌雄原核形成，并相互融合。

At the same time, the introduction of proportional solenoid control valve core part of the location of the pilot, the design specifications for valves used in Path parameter for the 32mm, the largest flow 480L/min, I rated the work of oil into the pressure of 31.5MPa, I rated the oil pressure to 30.5MPa.

同时引入比例电磁铁控制先导部分阀芯位置，本设计所采用的阀门规格为通径参数为32mm，最大流量为480L/min，进油口额定工作压力为31.5MPa，出油口额定工作压力为30.5MPa。

The results showed that the best effect is obtained with 1.6% protease when treating for 40 min.

实验结果表明，蛋白酶用量为1.6%，时间为40 min的处理工艺，对纤维的处理效果最好。

The pugging process experiment of silica brick pug was made in Φ1 600×450 wet pan mill to study the change rule of main component mixing homogeneity and molding performance of the pug. The results showed that the pervasion and mixing concurred when diversified materials acceded to the wet pan mill, prolonging time in dry-mixed and wet-mixed periods had no action for increasing mixing homogeneity with pugging time prolonging, the molding performance was improved and the porosity of silica brick adobe had downtrend, but prolonging time after pugging 14 min the effect was not remarkable.

通过对Φ1 600×450湿碾机混练硅砖泥料过程中泥料主要成分的混合均匀度、泥料成型性能的变化规律研究，发现在各种物料加入湿碾机时，同时存在着扩散混合作用，延长干混、湿混阶段的时间，对于提高混合均匀度实际上没有意义；随捏练时间延长，泥料成型性能改善，砖坯气孔率呈下降趋势，但捏练14 min以后继续延长捏练时间，采用正常压制制度时，砖坯气孔率降低值已经很小。

When the churning speed ranges from 200 to 500r/min, the pulverization of the granulation products will not change apparently.

当搅拌速度在200～500 r/min变化时，造粒产品的粉化率没有明显变化。

Rat experimental ulcers were induced by stress(swimming in cold water for 30 min), administration of acetic acid or indomethacin, and ligation of gastric pylorus.

大鼠溃疡造型用应激法(冷水中游泳30 min)、醋酸法、消炎痛法和幽门结扎法诱导产生。

Agitation system of pyrolusite pulp: a ratio of the agitation diameter to reactor diameter : d/D= 0.35; a ratio of distance of agitation from the bottom of reactor to agitation diameter : y/d=0.5; agitation rate of 500～600r/min.

矿浆搅拌系统：采用上旋式搅拌器；搅拌器的直径d与反应器直径D之比为d/D=0.35；搅拌器下端离反应器底部的距离y与搅拌器直径d之比为y/d=0.5；搅拌转速500-600r/min。

I can not make it blossom and suits me

我不能让树为我开花

When temperatures are above approximately 80 °C discolouration of the raceways or rolling elements is a frequent feature.

当温度高于 80 °C 左右时，滚道或滚动元件褪色是很常见的特征。