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The mutant was cultured on the rotating shaking bed at the speed of 200 r/min under 25℃for 72 hours,with inoculum size 8% and medium volume 40 mL/500 mL.

菌株于25℃在200 r/min的旋转式摇床上振荡培养72 h，接种量为8%，装液量为40 mL/500 mL三角瓶。

MATERIALS: A total of 60 healthy Sprague-Dawley rats were randomly divided into 6 groups, with 10 rats per group: normal control, diabetes mellitus control, exercise intensity 15, 20, 25, and 30 m/min.

材料： 将60只健康SD大鼠随机分为正常对照组、糖尿病模型对照组、糖尿病模型运动强度15，20，25，30 m/min组，每组10只。

The rats in group C were raised without any special interposal as control group. On the 14th day of the experiment, all rats in 3 groups received esophageal acid perfusion with 0.1 mol/L HCl, the drops was kept at 37℃ and 10 mL/h, the total duration lasted 50 min.

实验第14天，对A、B、C组动物分别进行食管酸灌注，使用0.1 mol/L盐酸滴注，滴注液保持37℃，速度10 mL/h，共50 min。

At 5 min after intracerebrally injecting [3H] - Glu , recovery of [3H] - Glu in control rat brain was 23.83%.

测得脑缺血10 min再灌2 h后，[3H]-Glu的回收率13.13％，显著低于对照组(P.05)，表明脑缺血再灌促进Glu的外排。

Cd〓 (1, 10, 100, 1000μM) stimulated directly GtH secretion from pituitary of common carp in vitro, with the highest GtH secretion at 100μM, GtH secretion stimulated with 1000μM Cd〓 was not higher than that stimulated with 100μM Cd〓.The antagonism between Ca〓 and Cd〓 in perfusion of the pituitary fragments was not obvious.After being preperfusion with 100μM Cd〓(with or without Ca〓), the GtH secretion of the pituitary fragment was irresponsive to 2-min pulse stmulation of 10μM sGnRH-A.

鲤鱼脑垂体碎片离体灌流孵育实验结果显示，Cd〓（1，10，100，1000μM）可直接刺激鲤鱼脑垂体分泌GtH，灌流液中Ca〓对Cd〓的拮抗作用不明显，100μM Cd〓刺激脑垂体分泌GtH达到高峰，Cd〓浓度增加到1000μM时，脑垂体分泌GtH并没有进一步增加，甚至有减少的趋势；100μM Cd〓预灌流脑垂体后，可使脑垂体对2-min 10μM sGnRH-A刺激的反应性消失；1.25mM的EDTA可消除100μM Cd〓刺激脑垂体分泌GtH的作用；TFP和Cd〓同时灌流脑垂体一段时间后，撤除TFP可使脑垂体分泌GtH显著增加，即出现"反跳"现象。

Results The tiopronin in situ gel of 0.2 % sodium hyaluronate had proper gelating temperature and certain sustained releasing ability, Papp and retention time of which was 15.43 cm·s-1 and 130 min respectively, irritancy and stability were up to standard of ophthalmic preparation.

结果 含有质量分数0.2 %透明质酸钠的硫普罗宁原位凝胶具有适宜的胶凝温度和一定的缓释作用，其角膜表观透过系数为15.43 cm·s-1，角膜前滞留时间为130 min；刺激性、稳定性符合眼用制剂要求。

He optimal process parameters are isothermal temperature range from 570 ℃ to 600 ℃ and holding time for less than 10 min.

iB2/Al-4Cu复合材料合适的半固态重熔参数为：加热温度570～600 ℃，保温时间小于10 min。

The present paper covers a simple method for preparing urease from jack bean. In terms of the method, the total yield was 37. 1% and the specific activity was 43. 6 μmol ?min-1/ mg protein.

报道了以刀豆为原料制备脲酶的一种简便方法，其总收率为37.1％，比活达43.6μmol·min~(-1)/mg蛋白。

The results show that when the mass ratio of sodium jarosite residue to anthracite is 5∶1, reduction roast lasts at 800℃for 0.5 h, leaching is carried out at 70℃for 40 min by 0. 5 mol/L H2SO4 with liquid-solid ratio of 7∶1, 93% of both iron and nickel can be leached out.

研究结果表明：当黄钠铁矾渣与无烟煤按质量比为5∶1均匀混合，在800℃还原焙烧0.5 h，焙烧渣用0.5 mol/L硫酸溶液按液固比7∶1在70℃浸出40 min时，渣中93%的铁和镍进入浸出液中。

The NNP (10 mg/kg) or solvents was injected 10 min before ischemia via jugular vein.

NNP(10 mg/kg)或溶剂在缺血模型形成之前10 min经颈静脉给药。

The concept of equivalent rotationally rigidity is offered and the formula of rotationally rigidity is obtained.

主要做了如下几个方面的工作：对伸臂位于顶部的单层框架—筒体模型进行分析，提出了等效转动约束的概念和转动约束刚度的表达式。

Male cats normally do not need aftercare with the exception of the night after the anesthetic.

男猫通常不需要善后除了晚上的麻醉。