查询词典 Macaca rhesus

The RT-PCR product was inserted into pTG19-T vector and transformed into E. coli successfully. By blastn, the sequence results of Kunming mus musculus were in complete accordance with the conservative sequence of Genbank NR_003278 (791bp-1153bp). By Blastn in NCBI, the sequence with little difference among animals was confirmed to be conservative. After Blastn, fourteen complete CDS coding for different animals were chosen. According to VECTOR NIT 9.0 software, the similarities between Kunming mus musculus and bos taurus, homo sapiens, erinaceus europaeus, cricetulus griseus, sus scrofa, dasypus novemcinctus, rattus norvegicus, rabbit, equus caballus, macaca fascicularis, didelphis virginiana, monodelphis domestica and vombatus ursinus was 67%, 100%, 100%, 36%, 100%, 100%, 67%, 100%, 100%, 92%, 99%, 99% and 99%. In the phylogenetic tress constructed with the forteen 18S rRNA by Treeview, the Kunming mus musculus clustered with cricetulus griseus, sus scrofa and rabbit, which was nearer to cricetulus griseus and was most far away from macaca fascicularis.(3) After sencodary structure analyses of 18S rRNA of mus musculus, an oligonucleotide fragment for RNAi was designed and synthesized, which was transformed into plasmid, and restriction enzyme analyses and sequencing results should the expression plasmid pGPH1/ GFP/Neo-mouse-sh 18S rRNA were constructed for RNAi successfully.

结果①通过RT-PCR检测显示18S rRNA基因在小鼠卵巢组织和单个GV期、MⅠ期卵母细胞中均有表达，且在未成熟卵母细胞中，MⅠ期的表达明显强于GV期的表达；②RT-PCR产物克隆测序结果显示：昆明小鼠18S rRNA基因保守区序列与基因库序列[NR_003278保守区部分(791bp～1153bp)]完全一致；Blastn比对结果发现：在不同物种中差异较小，选出14种生物18S rRNA全序列经VECTOR NIT 9.0软件分析，提示昆明小鼠18SrRNA与牛、人类、刺猬、中国仓鼠、猪、犰狳、褐鼠、兔子、马、食蟹猴、负鼠、短尾猊、袋熊的18S rRNA的相似率依次为67%，100%，100%，36%，100%，100%，67%，100%，100%，92%，99%，99%，99%；Clustal 1.81和Treeview构建出的分子进化树表明：在上述14种生物中昆明小鼠与中国仓鼠进化关系最近，与兔子、猪聚成一簇，与食蟹猴进化关系最远；③根据18S rRNA二级结构设计并合成RNA干扰寡核苷酸片段，重组质粒经过限制性内切酶及测序表明成功构建了pGPH1/GFP/Neo-mouse-sh 18S rRNA干扰表达质粒。

The results showed that :(1) sex ratio of Macaca mulatta population in Neilingding Island was 1∶ 0.98 , in which infancy group was 1∶1.07 , juvenescent group was 1∶0.93 , middle age group was 1∶0.6, middle-old age group was 1∶0.57 , old age group was 1∶1.14 ;(2) age composition pattern of Macaca mulatta population in Neilingdi...

结果表明：( 1 )内伶仃岛猕猴种群雌雄性比为 1∶ 0 。98，各年龄组性比幼年组为 1∶ 1 。0 7，青年组为 1∶ 0 。93，中壮年组为 1∶ 0 。6 ，中老年组为 1∶ 0 。5 7，老年组为 1∶ 1 。1 4 ；( 2 )列出了猕猴种群年龄结构图，确定了年增长率为 6 。9%，种群数量的动态公式是 Nt=2 0 0 e0 。0 69t；( 3)预测了未来 1 0 a的猕猴种群的数量和发展，种群数量从 2 0 0 1年到 2 0 1 0年增长到 1 2 0 0头左右；( 4 )内伶仃岛猕猴种群发展的最适容纳量为 90 0～ 1 1 0 0头(平均 1 0 0 0头)。

OBJECTIVE:To study heroin-dependent macaca mulatta model through contrast to morphie-dependent Macaca mulatta.

目的：用吗啡依赖猴作对比，对海洛因依赖猴模型进行研究。

Objective To analyze the genetic polymorphism in Macaca mulatta and Macaca irus, in order to set up a method of genetic analysis between those two monkey species.

目的 分析恒河猴和食蟹猴群体间的遗传多样性，确立一种对恒河猴和食蟹猴种群个体的遗传鉴别方法。

Results It was able to analyze the DNA polymorphism using 15 polymorphic microsatellite loci between Macaca mulatta and Macaca irus. The allele numbers were more than 7, and there were differences in 11 allele numbers between the two groups.

结果 筛选的15个具有显著多态性的微卫星DNA位点对恒河猴和食蟹猴种群可以进行DNA多态性分析，其等位基因数目均在7个以上，且两群体间有11个位点的等位基因数存在一定的差异。

Objective: To study the change of mandibles with age of Taihang Macaca mulatta, providing information for the physical and medical experiment with Macaca mulatta.

目的：探讨太行山猕猴下颌骨随年龄变化的形态学改变，为太行山猕猴的体质学、医学实验、进一步确定其分类地位提供可靠的依据。

The Major histocompatibility complex of Macaca mulatta, also called Mamu antigen, is a gene cluster directly related to immune response. Evolution creates the highly polymorphic nature of the MHC genes, and this leads to changes in the number as well as the function of these genes in individual Macaca mulatto, and even the emergence of species-specific genes.

恒河猴是应用最广泛的非人灵长类实验动物，其MHC基因是一个庞大的与免疫功能密切相关的基因群，在进化过程形成了Mamu基因不同的存在状态，使得不同个体的Mamu基因在数量和功能上有所差异，同时有些个体还产生了特异性的MHC基因，它的多态性和免疫反应的复杂性相对应。

It will be feasible in setting up a genetic analysis method by using PCR-analyzed polymorphic microsatellite DNA loci between Macaca mulatta and Macaca irus.

利用这些多态性微卫星DNA位点建立一种有效鉴别恒河猴和食蟹猴种群遗传背景的方法具有一定的可行性。

Methods The DNA polymorphism was analyzed in 50 individuals of Macaca mulatta and 50 individuals of Macaca irus using 15 PCR-microsatellites DNA loci and the differences of allele numbers between those two species were compared.

利用聚合酶链反应扩增技术采用15个多态性微卫星DNA位点对50只恒河猴和50只食蟹猴个体进行了DNA多态性的分析，对比两群体间等位基因数目差异。

Furthermore, the sensitivity was 100.00% in nine species, including human cytomegalovirus, simian virus 40, mareks disease virus, rhesus lymphocryptovirus, Epstein Barr virus, xenopus laevis, canis familiaris, ovis aries and macaca mulatta.

与MiRscreen和其它六种分类器相比，SVMensembler_50在敏感性和特异性方面均具有最优的性能，对独立测试集的AUC值达到0.935，远高于MiRscreen和其它六种分类器。

The circulation, consumption and reproduction of the trilogy have not only testified to its historical significance and literariness, but also invested it with new meanings.

该三部曲的流通、消费与再生产不仅验证了其历史意义及文学性，而切还赋予它以新的意义。

If he thought that he could buy me off, he would be wrong.

如果他认为他可以收买我，那他就大错特错了。