查询词典 Macaca

The RT-PCR product was inserted into pTG19-T vector and transformed into E. coli successfully. By blastn, the sequence results of Kunming mus musculus were in complete accordance with the conservative sequence of Genbank NR_003278 (791bp-1153bp). By Blastn in NCBI, the sequence with little difference among animals was confirmed to be conservative. After Blastn, fourteen complete CDS coding for different animals were chosen. According to VECTOR NIT 9.0 software, the similarities between Kunming mus musculus and bos taurus, homo sapiens, erinaceus europaeus, cricetulus griseus, sus scrofa, dasypus novemcinctus, rattus norvegicus, rabbit, equus caballus, macaca fascicularis, didelphis virginiana, monodelphis domestica and vombatus ursinus was 67%, 100%, 100%, 36%, 100%, 100%, 67%, 100%, 100%, 92%, 99%, 99% and 99%. In the phylogenetic tress constructed with the forteen 18S rRNA by Treeview, the Kunming mus musculus clustered with cricetulus griseus, sus scrofa and rabbit, which was nearer to cricetulus griseus and was most far away from macaca fascicularis.(3) After sencodary structure analyses of 18S rRNA of mus musculus, an oligonucleotide fragment for RNAi was designed and synthesized, which was transformed into plasmid, and restriction enzyme analyses and sequencing results should the expression plasmid pGPH1/ GFP/Neo-mouse-sh 18S rRNA were constructed for RNAi successfully.

结果①通过RT-PCR检测显示18S rRNA基因在小鼠卵巢组织和单个GV期、MⅠ期卵母细胞中均有表达，且在未成熟卵母细胞中，MⅠ期的表达明显强于GV期的表达；②RT-PCR产物克隆测序结果显示：昆明小鼠18S rRNA基因保守区序列与基因库序列[NR_003278保守区部分(791bp～1153bp)]完全一致；Blastn比对结果发现：在不同物种中差异较小，选出14种生物18S rRNA全序列经VECTOR NIT 9.0软件分析，提示昆明小鼠18SrRNA与牛、人类、刺猬、中国仓鼠、猪、犰狳、褐鼠、兔子、马、食蟹猴、负鼠、短尾猊、袋熊的18S rRNA的相似率依次为67%，100%，100%，36%，100%，100%，67%，100%，100%，92%，99%，99%，99%；Clustal 1.81和Treeview构建出的分子进化树表明：在上述14种生物中昆明小鼠与中国仓鼠进化关系最近，与兔子、猪聚成一簇，与食蟹猴进化关系最远；③根据18S rRNA二级结构设计并合成RNA干扰寡核苷酸片段，重组质粒经过限制性内切酶及测序表明成功构建了pGPH1/GFP/Neo-mouse-sh 18S rRNA干扰表达质粒。

The results showed that :(1) sex ratio of Macaca mulatta population in Neilingding Island was 1∶ 0.98 , in which infancy group was 1∶1.07 , juvenescent group was 1∶0.93 , middle age group was 1∶0.6, middle-old age group was 1∶0.57 , old age group was 1∶1.14 ;(2) age composition pattern of Macaca mulatta population in Neilingdi...

结果表明：( 1 )内伶仃岛猕猴种群雌雄性比为 1∶ 0 。98，各年龄组性比幼年组为 1∶ 1 。0 7，青年组为 1∶ 0 。93，中壮年组为 1∶ 0 。6 ，中老年组为 1∶ 0 。5 7，老年组为 1∶ 1 。1 4 ；( 2 )列出了猕猴种群年龄结构图，确定了年增长率为 6 。9%，种群数量的动态公式是 Nt=2 0 0 e0 。0 69t；( 3)预测了未来 1 0 a的猕猴种群的数量和发展，种群数量从 2 0 0 1年到 2 0 1 0年增长到 1 2 0 0头左右；( 4 )内伶仃岛猕猴种群发展的最适容纳量为 90 0～ 1 1 0 0头(平均 1 0 0 0头)。

So welcome, let's give a welcome to Macaca ,here.

那么欢迎，让我们在这里欢迎Macaca。

So welcome, let's give a welcome to Macaca, you know.

他说：来，让我们欢迎 Macaca 。

R. Sidarth "macaca," which in some places means "monkey."

Sidarth为&macaca&，这个词在某些地方有&猴子&的意思。

Sidarth "macaca", which in some places, means, m onkey .

Sidarth为&macaca&，这个词在一些地方是猴子的意思。

But despite the notoriety of tactics like the macaca video, he says the Internet is still only one part of a broader communications strategy.

但是他表示尽管出现了象macaca录像这类利用因特网传播丑闻的战略，因特网还只是更广泛的交流策略的一部分。

At a rally Allen called him macaca and added "welcome to America".

在一次集会上， Allen 称他自己为 macaca 并且说，欢迎来到美国。

But despite the notoriety of tactics like the macaca video, he says the Internet is still only one part of a broader communications strategy.

尽管还存在 macaca 视频这样不良例子，但他说互联网仍是更广泛的交流战略的一部分。

Sidarth "macaca," which in some places means "m onkey ."

Sidarth 为&macaca&，而这一词汇在某些地方是&猴子&的意思。

As she looked at Warrington's manly face, and dark, melancholy eyes, she had settled in her mind that he must have been the victim of an unhappy attachment.

每逢看到沃林顿那刚毅的脸，那乌黑、忧郁的眼睛，她便会相信，他一定作过不幸的爱情的受害者。

Maybe they'll disappear into a pothole.

也许他们将在壶穴里消失