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Using 100% ethanol and ice cold acetic acid to make stock solutions recommended by your company doesnot give any problem for the product to be dissolved. However, because limonin slightly dissolves in water, is it possible that there will be crystal of the product when we prepare the working solution from the stock while exceeding the solubility. If there is such effect on the research result, are there any solutions for it? According to research articles, the IC50 of limonin in cell model is around 100ug/ml, the effective concentration in animal model are way higher!

产品用贵公司推荐的100％乙醇或冰醋酸作为融媒配成母液时并不存在溶解的问题，但当母液进一步配制工作液时由于Limonin微溶于水，若超出溶解度范围是否将有结晶析出，如有将会影响研究结果，应该如何解决该问题（注：根据文献报道细胞模型Limonin的IC50约在100ug/ml左右，动物模型有效作用浓度将更高！

The antioxidation of DPPH, IC50 value was 3.78、23.19、24.87、11.77 and 14.62 mg/ml, Rhus javanica Linne showed the most potent effect.

且其清除DPPH自由基之活性，IC50分别为为3.78、23.19、24.87、11.77及14.62 mg/ml，其中以五倍子效果最好。

ResultsThe optimum condition was extracting pueraria lobata three times for three hours with 35 ml 80%ethanol as solvent at 100℃.

结果溶剂为80%乙醇；温度100℃；溶剂体积35 ml；提取时间及次数为3。

Methods The logarithmically growing CEM cells cultured in vitro ( cell density 2 × 105/mL) were exposed to 0.1, 0.5, 1, 5, and 10 μM Dex, then were collected 24, 48 and 72 hrs later. Untreated CEM cells were used as Controls. The cell viability was determined by trypan blue dye exclusion.

方法将在体外培养的对数生长期的CEM细胞浓度调至2×105个/mL，接种于24孔培养板中，用终浓度分别为0.1，0.5，1，5，10 μM地塞米松处理，以不加任何药物的CEM细胞作为对照组，培养后24，48，72 h取样。

The application of low pressure boiling technique could increase wort boiling temperature to 100.5 ℃ and advance protein agglomeration and reduce solidifiable nitrogen content to 0.5 mg/100 mL. Maillard reaction and Brown reaction were strengthened in the boiling, melanoidin and melanoid compound contents increased, wort chromaticity improved, and the oxidation resistance of wort strengthened. Besides, the boiling intensity dropped from 9 %～12 % to 7 %～8 % and boiling time shortened to 10～20 min, which improved production efficiency and saved 20 % energy and improved beer non-biological stability.

采用低压煮沸工艺，可提高煮沸麦汁温度至100.5 ℃，加强蛋白质凝聚，麦汁可凝固氮下降0.5 mg/100 mL；煮沸过程加强了美拉德反应、&棕色反应&，类黑精、类黑素化合物增加，麦汁色度增加，麦汁的抗氧化能力增强；煮沸强度从9 %～12 %降至7 %～8 %，煮沸时间缩短10～20 min，提高生产效率，节约能源20 %；改善啤酒非生物稳定性。

The results show that by providing wastewaters pH of about 3 and reaction time of 2 hours, the quantity of H2O2 of 40 mL/L, the concentration of ferrous iron is 0.4 g/L, the effluent of microelectrolysis goes into Biological contact oxidation process, in which DO, HRT and volume load are 4.5 mg/L, 10 hours and 1.0 kg CODCr/(m3·d) respectively, satisfying the first grade of the national standard.

结果表明，在废水pH值为3左右、反应时间2 h的前提下，双氧水投加量40 mL/L，二价铁离子质量浓度为0.4 g/L，再经过微电解处理后的出水进入接触氧化阶段。在溶解氧为4.5 mg/L左右，水力停留时间为10 h、容积负荷1.0 kg CODCr/（m3·d）左右的条件下，出水CODCr小于100 mg/L，可达到国家对丙烯腈废水处理要求的一级标准。

In vitro this oil showed a better antifungal activities, under the concentration of 2.0 μL/mL in media it could totally inhabit the growth of Crytococcus neoformans, S porothrix schenckii, Microsporum lanosum, M. Gy pseum and Chaetomium globosum.

该挥发油具有一定的体外抗真菌活性，在培养基油浓度小于2.0 μL/mL的情况下，能够完全抑制新型隐球菌、申克氏孢子丝菌、羊毛状小孢子菌、石膏样小孢子菌和球毛壳霉等皮肤真菌的生长繁殖。

PCC6803. In the enclosed photobioreactor, after 58. 5h mixotrophic cultivation, 2. 50g/L cell density, 1. 0g/L. d productivity, 15. 3μg/ml chlorophyll concentration and 25. 3%energy efficiency were achieved, which were respectively 8. 9 times, 11times, 2. 3 times and 4. 9 times as much as those in photoautotrophic culture.

在封闭式光生物反应器中，混合营养培养58.5小时，集胞藻6803藻细胞密度达到2.50g/L，是同期光自养的9倍，藻细胞产率为1.0g／L.d，是光自养培养的11倍，叶绿素浓度为15.3μg／ml，是光自养的2.3倍，能量得率为25.3％，是光自养培养过程的5倍。

First take the administration team group first forIM model making by given ranitidine 0.06g and the 5％hyperhaline typical finemixtured powder with the 50ug/ml MNNG, 0.1％ammonia water,2g sodiumsalicylate added into 100ml 30％alcoholic solution for intragastric administration 6weeks and the nomal control group by given the ordinary feeds and commonwater;After 9 weeks administration,the model groups rats was put to death for modelmaking detection.

从实验第一天起正常对照组给予普通饲料和正常饮水；模型组大鼠则食用含0.06雷尼替丁和5％的高盐的标准粉末状混合饲料，给予含有浓度为50μg／ml的甲基硝基亚硝基胍溶液每日自由饮用，MNNG溶液装入涂有黑漆的避光饮水瓶内，每24 h更换1次药液，每周饮5天，周2和周5则给予浓度0.1％的氨水饮用；同时将2g水杨酸钠放入100ml 30％的酒精溶液中，配置成2％的水杨酸钠和30％的酒精的混合溶液，从第4周起每天早上9点灌胃。

The chromatographic conditions were as followed: C8 column, 0.045 mol/L ammonium phosphate monobasic solution∶acetonitrile (2∶1)(adjusted to pH7.35 with triethylamine) as mobile phase, temperature: 40℃, the flow rate: 1.0 ml/min, detection wavelength: 210nm.

采用C8柱，以0.045mol/L磷酸二氢胺溶液∶乙腈(2∶1)(用三乙胺调pH至7.35)为流动相，流速1ml/min，检测波长210nm，柱温40℃。结果阿奇霉素的线性范围为50.3～1006μg/ml，平均回收率100.1%，r=1.0000，RSD=0.8%(n=9)。

On the other hand, the more important thing is because the urban housing is a kind of heterogeneity products.

另一方面，更重要的是由于城市住房是一种异质性产品。

Climate histogram is the fall that collects place measure calm value, cent serves as cross axle for a few equal interval, the area that the frequency that the value appears according to place is accumulated and becomes will be determined inside each interval, discharge the graph that rise with post, also be called histogram.

气候直方图是将所收集的降水量测定值，分为几个相等的区间作为横轴，并将各区间内所测定值依所出现的次数累积而成的面积，用柱子排起来的图形，也叫做柱状图。