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Guangdong college of pharmacy,guangzhou,guangdong 510006,china;henan university of traditional chinese medicine,zhengzhou,henan 450008,chinaabstract:objective to establish a method for the determination of the content of total alkaloid in nao mai tong recipe.methods acid dye colorimetry was used in the determination with bromocresol green buffer solution as the color developing agent with absorbance was determined at the detection wavelength of 417 nm.results the calibration curve of the absorbance was linear with the concentration of ligustrazine in the range of 0.031～0.153 mg/ml(r=0.9998).the average recovery of ligustrazine was 100.95％,rsd=1.75%.

作者单位：广东药学院中药化学与分析教研室，广东广州 510006；河南中医学院，河南郑州 450008 目的建立脑脉通有效部位中总生物碱的含量测定方法。方法采用酸性染料比色法，以溴甲酚绿缓冲液作为生物碱的显色剂，测定样品溶液在417 nm波长处的吸收度。结果对照品川芎嗪质量浓度在0.031～0.153 mg/ml的范围内与吸光度呈良好的线性关系，r=0.9998。平均回收率为100.95％，rsd为1.75％。精密度和稳定性良好。

We did bronchovesicular lavage with 10 mL normal saline, then collected all cells in BALF, stained cells by Giemsa's stain and counted the total number of cells and differential cells numbers for observing the inflammatory changes in lungs after RSV infection.

以10 ml生理盐水进行支气管肺泡灌洗，收集肺泡灌洗液中细胞，常规吉姆萨染色，行细胞分类计数，观察RSV感染对肺脏的炎症影响。

A determination method of ursolic acid in Callicarpa macrophylla Vahl leaves by reversed- phase high performance liquid chromtograph was established. The chromatographic column (Symmetry Shield RP18), methanol-water mobile phase(88:12, V/V) with 1.0 ml/min flow rate, the detection wavelength (210nm) and the column temperature (25℃) were adopted.

建立反相高效液相色谱测定其含量的方法，色谱柱为Symmetry Shield RP18柱，流动相为甲醇-水（体积比88∶12），流速1.0 ml/min，检测波长210 nm，柱温25℃。

A determination method of ursolic acid in Callicarpa macrophylla Vahl leaves by reversed- phase high performance liquid chromtograph was established. The chromatographic column (Symmetry Shield RP18), methanol-water mobile phase(88:12, V/V) with 1.0 ml/min flow rate, the detection wavelength (210nm) and the column temperature (25℃) were adopted.

建立反相高效液相色谱测定其含量的方法，色谱柱 Symmetry Shield RP18柱，流动相为甲醇-水（体积比88∶12），流速1.0 ml/min，检测波长210 nm，柱温25℃。

AFGF (5 ng/ml) sustained the growth of calvaria cells in culture during multiple passages (72 days) at 0.2% serum.

aFGF(5 ng/ml)在文化在多样的通过期间支撑了头顶细胞的生长(72天)在0.2%浆液。

Newborn rat calvaria osteoblastic cells were incubated with TNF-α(20、30、40ng/ml) for 24 and 48h.

用TNF-α 20、30、40ng／ml的量刺激成骨细胞，观察24、48小时，测定细胞培养液中亚硝酸根含量。

The carboxyl groups of QDES were used to couple the free amino groups of EAH Sepharose 4B with the carbodiimide coupling method, and immobilized density was 5μmol/ml.

通过碳二亚胺反应将四肽配基QDES固定在商品介质EAH Sepharose 4B上制备出了配基固定化密度为5μmol/ml的亲和吸附介质，并利用亲和色谱柱考察了其吸附蛋白质特性。

The isolate grew well in R2 liquid medium and could pass through 0.22 μm and 0.45 μm filtrate membranes. The colony was grain-like in solid medium. Through electron microscopy, the isolate YY0801 exhibited helicity during their exponential growth phase. The isolate YY0801 was able to ferment glucose and D-fructose and to catabolize arginine, but did not to hydrolyse urea. The isolate was resistant to ampicillin (2000 U/mL).

分离菌株在R2液体培养基中生长良好，能通过孔径为0.22 μm、0.45 μm的微孔滤膜；在R2固体培养基上呈颗粒状菌落；在对数期呈典型的螺旋状；能利用葡萄糖、D-果糖作为碳源；能强烈代谢精氨酸；不能利用尿素，在含氨苄青霉素钠(2000 U/mL)的R2液体培养基中生长良好。

The optimal determining conditions were obtained: adding quantity of a monolayer medium 15ml, the suspension concentration of Micrococcus luteus 109 cfu/ml, the adding quantity of Oxford Plate 100μl, agar concentration 1%, the concentration of Na2HPO4 1％, and the pH of the medium adjusted to 7.0. The medium was optimized by single factor and the orthogonal experiments on the basis of the centrifugate of distiller's grains. The optimal composition was obtained as follows: sucrose 0.6, peptone 0.1, yeast extract 0.2, K2HPO4?

以管碟法为基础，对影响Nisin效价测定的各种主要因素进行了分析，并得出了Nisin效价测定的最佳条件：采用单层培养基（每个平板加量为15ml），检测培养基中Na2HPO4·12H2O浓度改为1％，菌悬液浓度109cfu/ml，不加吐温20，琼脂浓度1％，培养基pH7.0，牛津杯加液量100μl ，30℃培养24小时。

Results: We found that sVCAM-1 (1 to 10 mug/ml) induced eosinophil chemotaxis, rather than chemokinesis, in a concentration-dependent fashion.

结果： sVCAM-1（1-10 mug/ml）能诱导嗜酸性粒细胞的趋化性，而不是化学激活，且这种趋化为浓度依赖性。

On the other hand, the more important thing is because the urban housing is a kind of heterogeneity products.

另一方面，更重要的是由于城市住房是一种异质性产品。

Climate histogram is the fall that collects place measure calm value, cent serves as cross axle for a few equal interval, the area that the frequency that the value appears according to place is accumulated and becomes will be determined inside each interval, discharge the graph that rise with post, also be called histogram.

气候直方图是将所收集的降水量测定值，分为几个相等的区间作为横轴，并将各区间内所测定值依所出现的次数累积而成的面积，用柱子排起来的图形，也叫做柱状图。