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Porphyridium cruentum were treated with some antibiotics which were used as genetic selected marks in genetic engineering. The results showed that the algae cells could resist high concentration of Penicillin,Streptomycin,Gentamicin and Kanamycin, without any influence at the dose below 100 u/mL of which can kill bacterium adhere to the algae cells . These antibiotics can be used to repress bacterium in purifying algae strain.Porphyridium cruentum is high sensitive to Erythromycin,Terramycin and Ofloxacin....

研究了基因工程中常用抗生素标记对紫球藻的生物学效应，结果表明紫球藻对青霉素、链霉素、庆大霉素以及卡那霉素较不敏感，剂量在100u/mL以下对其生长无显著影响，但能抑制或杀灭伴生的杂菌，因此这些抗生素可用于藻种纯化过程中抑菌；紫球藻对红霉素、氧氟沙星和土霉素非常敏感，低质量浓度就能杀死藻细胞，可用作基因工程或遗传育种的抗药性选择标记；对四环素和氯霉素较为敏感，明显抑制细胞生长，并呈浓度梯度效应。

RESULTS: The best linearity of test was between 0.5-10 ng/mL. The intra-assay coefficient of variation was 8.17% and inter-assay CV, 9.72%.

结果：该方法最佳线性范围：ASP浓度0.5～10ng/mL，平均批内和批间变异系数分别为8.17%和9.72%。

Pf7-14 was chosen to study the spatial-temporal colonization patterns on both rice flag leaves and rice stems under greenhouse conditions by spraying 1×10〓 cfu/ml bacterial suspension of Pf7-14 at late booting stage, periodical sampling, and recovering the bacteria through diluting-plate method as well as graphic test and the Shapiro-Wilk test for normality of the data.

以菌株Pf7-14为代表，在温室条件下，在水稻孕穗后期喷雾浓度为1×10〓cfu/ml的菌悬浮液，然后定期取样，用稀释平板法在含抗生素Nal的培养基上回收细菌，并通过图形测试和Shapiro-Wijk正态测试，测定了该菌株在水稻剑叶和茎基部的时间、空间定殖型。

Methods:SGC7901 cells were cultured with paclitaxe at different concentrations (1, 5, 10, 20, 40μg/ml) and different time (12, 24, 36, 48h). Methyl thiazolyl tetrazolium assay was used to measure the anticancer activity of paclitaxe.

采用MTT法检测不同浓度(1、5、10、20、40μg/ml)，不同时间(12、24、36、48h)下多西紫杉醇的抗癌活性并计算其亚毒性剂量。

The proposed method could determine trace thallium as low as 0.02ng/mL.

本方法可测定水中低达0.02ng/mL的铊。

Results The titer of lentivirus was 6×10^5IU/mL, transduction efficiency ranged from 6~11%.

结果 包装的慢病毒滴度达6×10^5IU/mL，对J558L细胞的感染效率为5%~10%。

The titre of virus was 1.3×1011 pfu/mL.

病毒滴度为1.3×1011pfu/mL。

The titre of purified Adenovirus vector was up to 10〓pfu/ml after packaging and expanding in 293 cells.

重组腺病毒载体在293细胞中包装后可以得到滴度高达10〓pfu／ml的病毒液。

Results The three plasmids were effectively cotransfected and a high titre of lentivi-rus was obtained (2×10 6 IU/ml).

结果共转染293T细胞后获得较高滴度的慢病毒(2×106IU/ml)。

OBJECTIVE: We assessed the pharmacokinetics and tolerability of 5 mg loratadine syrup (1 mg/mL) in children aged 2 to 5 years.

目的：评价5 mg氯雷他定糖浆(1 mg/mL)在2到5岁儿童体内的药代动力学和耐受性。

On the other hand, the more important thing is because the urban housing is a kind of heterogeneity products.

另一方面，更重要的是由于城市住房是一种异质性产品。

Climate histogram is the fall that collects place measure calm value, cent serves as cross axle for a few equal interval, the area that the frequency that the value appears according to place is accumulated and becomes will be determined inside each interval, discharge the graph that rise with post, also be called histogram.

气候直方图是将所收集的降水量测定值，分为几个相等的区间作为横轴，并将各区间内所测定值依所出现的次数累积而成的面积，用柱子排起来的图形，也叫做柱状图。