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Rat in Group B was set an air bladder which was empty and not perfused water. Water of 0.6 ml, 0.8 ml, 1.0 ml, 1.2 ml, 1.4 ml and 1.5 ml was perfused respectively into the air bladders which were set in the colon of rats in group C, D, E, F, G and H. Then the changes of ethology of the rats were recorded in interval of an hour.

应用疼痛行为学的观察方法，观察1h各组动物的行为学改变，并采用免疫荧光化学的方法，观察大鼠DCN中神经元被激活的标志c-fos及胶质细胞的胶质原纤维酸性蛋白(glial fibrillary acidic protein， GFAP)和小胶质细胞特异性补体OX42的表达情况，空白对照组是在疼痛高峰点(1.2 ml)处死取材。

Our data revealed glucose and beef extract to be the most influential parameters, and the composition of the optimal fermentation medium (100 mL) was determined to consist of 4.46 g glucose, 2 g soluble starch, 4 g silkworm powder, 2.93 g beef extract, 0.10 g KH2PO4 and 0.15 g MgSO4·7H2O.

最后通过中心组合设计和响应面分析法，确定了主要影响因子的浓度。优化后的培养基组成为：葡萄糖4.46 g/100 mL，可溶性淀粉2 g/100 mL，蚕蛹粉4 g/100 mL，牛肉膏2.93 g/100 mL，KH2PO4 0.1 g/100 mL和MgSO4·7H2O 0.15 g/100 mL。

Result : 1. E xperimental study:(1)After 48 hours culture,the morphology of K562 cells that FFJZ's concentration is 1mg/ml and less than 1mg/ml became fusiform and claviform.The K562 cells that FFJZ's concentration is 2mg/ml has no conspicuous different.The K562 cells that FFJZ's concentration is 4mg/ml and 6mg/ml took on shapes of crenation.

结果：1、体外实验：（1）K562细胞经FFJZ作用48小时后，浓度在 1mg/ml及以下组出现细胞形态呈梭状、棒状，伴有胞浆丝状突起形成伪足，2mg/mlFFJZ组细胞与对照组无明显差别，4mg/ml及6mg/mlFFJZ组，细胞出现皱缩、破碎； 8mg/mlFFJZ及10mg/mlFFJZ组细胞经培养24小时后，即出现细胞明显变小，且均匀分散，以后时间里均呈现此种状况。

The results showed that 1 there were no significant differences in the rates of cytoplast protrusion and enucleation between oocytes that were incubated in colchicine (0.4 μg/mL) for 0.5 h and oocytes that were incubated in colchicine (0.4 μg/mL) for 1 h, and the rate of cytoplast protusion can be 85.4% while the rate of cytoplast enucleation is 100%. 2 There was no significant difference in oocyte enucleation between oocytes treated with medium containing 0.2 μg/mL colchicine for 0.5 h and oocytes treated with medium containing 0.4 μg/mL colchicine for 0.5 h. 3 A maturation time of 18–23 h did not affect the rates of cytoplast protusion and enucleation by chemically assisted enucleation, whereas the rate of enucleation of oocytes by blind enucleation was found to decrease with a prolonged incubation time. 4 The development rates of reconstructed embryos could not be influenced by these two enucleation methods, increased from oocytes matured for 21–23 h.

结果表明： 1 卵母细胞在0.4 mg/mL的秋水仙素溶液中分别孵育0.5 h和1 h，胞质突起率和去核率没有显著的差异，突起率可高达85.4%，去核率达到100%； 2 0.2 mg/mL或0.4 mg/mL秋水仙素溶液将卵母细胞处理0.5 h，对去核效果没有显著影响； 3 对于体外成熟18~23 h的卵母细胞，随着成熟时间的延长，盲吸法的去核率降低，但没有影响秋水仙素诱导胞质突起的比率和去核率； 4 两种去核方法对重构胚的发育没有产生显著影响，但成熟21~23 h卵母细胞重构胚囊胚的发育率显著高于成熟18~20 h卵母细胞重构胚囊胚的发育率。

The high rate of protoplasts formation and fusants regeneration could been obtained for strain F46 lysing 75min with 10 mg/mL snailase and 10 mg/mL lysozyme (1:1) in 35℃water bath, and for strains SC1 and SC11 lysing 60 min and 100min with 10 mg/mL lysozyme and 15 mg/mL lysozyme respectively in 35℃water bath.

对菌株SC1和SC11，分别用10mg/mL和15mg/mL溶菌酶酶解60min和100min较合适；对菌株F46，以蜗牛酶(10mg/mL)和溶菌酶(10mg/mL)体积比为1：1的混合酶液酶解75min为宜。

Methods According to the 4×4 Latin Square design,lytic efficacy was assessed in UK dose[(10 000 U/ml,20 000 U/ml,30 000 U/ml or 40 000 U/ml)]and the timing of Uk infusion(2,4,8,16 h after blood sample taken)and UK lytic time(2,4,8,16 h after UK infusion)in vitro.15 rabbits were subjected to the intracerebral hematoma model for analysing the effect of 2 differents UK dose(10 000 U vs 30 000 U)on brain tissue around hematoma cavity.

目的 探讨尿激酶溶解血凝块的最佳用药剂量、用药时机及药物最佳作用时间，为临床一次性清除血肿提供实验依据。方法采用2个(4×4)拉丁方设计，分析UK剂量[(1万U/ml、2万U/ml、3万U/ml、4万U/ml)]、用药时机(抽血后2、4、8、16 h)和药物作用时间(加UK后2、4、8、16 h)对溶凝效果的影响。

Plasma IL-2 level was (64.3±32.3) pg/ml and (200.0±50.0) pg/ml and sIL-2R (54.9±33.3) U/ml and (167.5±89.0) U/ml in the patients with heat apoplexy and in controls, respectively, with very significant difference.

高温中暑组IL-2为（64.3±32.3） pg/ml，sIL-2R为（54.9±33.3） U/ml；而对照组IL-2为（200.0±50.0） pg/ml，sIL-2R为（167.5±89.0） U/ml，差异均有非常显著性。

Esults LVSV and RVSV determined by RT-3DE were (15.90±10.62) ml and (12.42±4.69) ml respectively, which were shown good correlation with those determined by MRI [(16.56±10.64)ml and (13.96±7.96) ml]. The r value was 0.98, and 0.75, respectively. LVSV and RVEV determined by RT-3DE were (12.67±10.10) ml and (11.83±12.02) ml respectively, which were well correlated with MASE (8.17±1.92)mm and TASE (11.04±1.06) mm determined with ME. The r value was 0.86 and 0.88 respectively.

T-3DE测定左室每搏量(12.67±10.10)ml和ME测定二尖瓣瓣环收缩期平均位移(8.17±1.92)mm之间的相关性显著，相关系数r=0.86(P.001)；RT-3DE测右室每搏量(11.83±12.02)ml和ME测定三尖瓣瓣环收缩期位移(11.04±3.06)mm两者亦存在显著正相关，相关系数r=0.88(P.001)。

It was found that the peak urine flow rate increased from 7.6 ml/s to 20.1 ml/s, the residual urine volume decreased from 126.5 ml to 16.3 ml, and the ipss decreased from 24.1 to 5.6 points in 6 months.conclusion tups as a good treatment for bph is effective, highly safe and causing few complications.

最大尿流率由术前7.6 ml/s升到术后20.1 ml/s(术后6个月)，剩余尿量由术前126.5 ml减至术后16.3 ml，国际前列腺症状评分由术前24.1分降至术后5.6分。结论经尿道前列腺裂开术治疗前列腺增生，具有安全性高、并发症少、疗效好的优点。

Results MIC90 values of secnidazole, Tinidazole and meronidazole to 120 strains of Gram-negative anaerobic bacteria were 1 - 4, 1 - 2 and 4- 8 μg/ml, and were 4 - 8, 4 - 16 and 16 - 32μg/ml to 80 strains of Gram-positive anaerobic bacteria, respectively.

结果塞克硝唑、替硝唑和甲硝唑对120株革兰阴性厌氧菌的MIC90分别为1～4μg/ml、1～2μg/ml和4～8μg/ml，对80株革兰阳性厌氧菌的MIC90分别为4～8μg/ml、4～16μg/ml和16～32μg/ml。

On the other hand, the more important thing is because the urban housing is a kind of heterogeneity products.

另一方面，更重要的是由于城市住房是一种异质性产品。

Climate histogram is the fall that collects place measure calm value, cent serves as cross axle for a few equal interval, the area that the frequency that the value appears according to place is accumulated and becomes will be determined inside each interval, discharge the graph that rise with post, also be called histogram.

气候直方图是将所收集的降水量测定值，分为几个相等的区间作为横轴，并将各区间内所测定值依所出现的次数累积而成的面积，用柱子排起来的图形，也叫做柱状图。