查询词典 ML

The recombined yeast could grow on the cultures with the cellobiose as a sole carbon source, and the β-glucosidase activity achieved 0.764 U/mL after 48 hours' cultivation.

重组酵母可以在以纤维二糖为唯一碳源的培养基上生长， 48 h时b-葡萄糖苷酶酶活达到0.764 U/mL。

Serum free medium containing 0,16,32,64,128/μg/mL was added 24 hours after culture and cultured for another 24 hours. Cells were collected centrifugally and resuspended in 75% alcohol. Finally, cells were fixed 4℃overnight. Then cells were stained with PI for 30 min under 4℃.. FCM was run with parameter FL2 - A.

流式细胞仪检测细胞凋亡率对数生长期的BIU-87细胞接种于培养瓶内，24 h后更换含有终浓度0、16、32、64、12μg/mL的无血清培养液，处理24 h，离心收集，75%冷乙醇悬浮后4℃固定过夜，PI染液4℃避光染色30min，流式细胞仪于FL2-A参数下检测。

Methods: red sage root,astragalus root,white peony root,corydalis tuber,fevervine herb,dahongpao,chinese gall,cinnamon bark in this preparation were identified by tlc;the content of paeoniflorin in this preparation were determined by hplc, agilent zorbax sb 4.6×150 mm,rp-18e（ 5μm） was used. the mobile phase consisted of acetonitril-water ( 15:85 ), the velocity of flow was 1.00 ml.min-1.the detection wavelength was 230 nm.

采用tlc法鉴别处方中丹参、黄芪、白芍、延胡索、鸡屎藤、大红袍、五倍子、肉桂等药材；采用hplc法测定处方芍药苷含量，agilent zorbax sb 4.6×150 mm，rp-18e（ 5 μm ）色谱柱，流动相为乙腈-水（ 15∶85 ），流速1.00 ml.min-1，检测波长230 nm。

The research on the conditions of chlamydospore production for the liquid fermentation showed that, the better single factors were oat liquid media, 30℃, 120r/min surge culture, sucrose as carbon source,(NH_4)_2SO_4 as nitrogen source and the ratio of 20 to 1 for C/N. Under the better single factors mentioned above, the largest quantity of chlamydospore, i.e.3.05×10~7spores/ml, was obtained on the 10th day after culture.

木霉T-33菌株产生厚垣孢子的发酵条件研究表明，采用燕麦粉培养液、30℃、120r/min振荡培养、250ml三角瓶装入80ml培养液、接种5片活化2d的5mm直径的菌片，碳氮源选择蔗糖和硫酸铵，C/N为20/l，足适合T-33菌株产孢的最佳单因素条件(碳氮源实验基于Czapek培养基)；用这些最佳单因素组合(不包括碳氮源和C/N结果)培养木霉T-33绘制了产孢曲线，发现在培养第10d得到最大的厚垣孢子产量3.05×10~7个/ml。

Result In the first 4 d of experiment, the corrected mortalities of spore suspensions with various concn. of P. cicadae APC20 to P. xylostella were lower than 50%, but on the 7 th day, that of spore suspension with concn. of 3.0×10^8 spores/ml reached 85.11% and the insect corpses was bestrewed in the mycelium and spore powder of P. cicadae, indicating that APC20 showed stronger parasitic lethality to P.

结果］药后第1~4天，蝉拟青霉APC20各个浓度的孢子悬浮液对小菜蛾的校正死亡率均低于50%，而第7天，浓度为3.0×10^8个／ml的孢子悬浮液对小菜蛾的校正死亡率达85.11%，并且虫尸布满蝉拟青霉的菌丝体和孢子粉，说明APC20对小菜蛾呈现较强的寄生致死性。

Results The detecting rates of the displacement of the mitral coaptation point ,the flail partial mitral leaflets,the coarse and erratic flutter of flail leaflets, the flicking mitral chordae tendineae with coarse and erratic flutter and the vegetation by TEE and TTE with 2-D were 100%and 94.3%, 100%and 65.7%, 85.7%and 54.3%, 77.1%and48.6%, 100%and33.3%, respectively.With color Doppler imaging,eccentric wall jets or eccentric-centric jets were all detected by TEE and TTE, respectively. The regurgitant volume based on PISA method by TEE and TTE was(2025±402)and (607±268)ml/min,respectively.

结果 TEE和TTE对二尖瓣腱索部分断裂的切面超声征象包括收缩期瓣缘对合不良、局部链枷运动、局部粗大扑动、腱索断端飞鞭运动伴粗大扑动及赘生物的检出率分别为100%和94.3%、 100%和65.7%、 85.7%和54.3%、 77.1%和48.6%及100%和33.3%； TEE和TTE二尖瓣腱索断裂的彩色多普勒表现为偏心附壁返流束或偏心伴向心返流束，其检出率均为100%；根据PISA法由TEE及TTE估测的返流量分别为(2025±402)及(607±268)ml/min。

The optimized velocity of flow in dynamic adsorption is tenth of the volume of the resin columniation per minute and the adsorbed volume of the solution which contain 101μg/ml activity component of 2507 is 65 time of the volume of the resin columniation. The dynamic adsorption capacity of per gram resin is 9494μg/g, and the adsorption rate is almost 100％ because the hydrogen bond may be formed in the adsorption process.

动态吸附最佳的吸附流速为每分钟十分之一个柱体积，在此条件下，可以吸附相当于约65个柱体积的有效组分浓度为101μg／ml的溶液，每克树脂的动态吸附量为9494μg／g，因为可能在吸附过程中有氢键生成，所以吸附率几乎为100％。

Methods Twenty canines were treated by subcutaneous injection of 60% CCl4 colza oil emulsion into the back, at a dose of 1.0-1.3 mL/kg every 10 days for a total of 6-8 times.

犬20只，用60% CCl4菜籽油乳剂于犬背部皮下注射，1.0~1.3 mL/kg，每10天注射1次，共6~8次。

The results showed that:(1) we can mapping quantitative trait locus while estimating the variance component of QTL;(2) granddaughter design is better than daughter design when mapping QTL;(3) it is easy to map a QTL for trait with a high heriability and a large QTL variance contribution;(4) we can estimate the variance component of a QTL by TM-BLUP based on ML method whether the QTL has only 2 alleles or QTL has normal distributed alleles effects;(5) the estimation accuracy of variance component contributed by QTL was improved by using of grand daughter design;(6) the higher the heritability and the QTL variance contribution was, the more accurate estimation of QTL variance component.

结果表明：（1）采用随机QTL效应模型和最大似然法，在估计QTL方差组分的同时，能够定位QTL；（2）孙女设计与女儿设计相比，在其它因素相同时，容易检出QTL；（3）遗传力高，QTL方差贡献较大的性状，QTL检出的效果优于遗传力低，QTL方差贡献较小的性状；（4）无论QTL上有2个等位基因，还是QTL上等位基因的效应服从正态分布，都可将其看作随机效应，采用基于TM-BLUP的ML法，估计其方差组分和定位QTL；（5）QTL方差组分估计的准确性，孙女设计高于女儿设计；（6）遗传力高的性状，QTL方差贡献大的QTL，QTL方差组分估计的准确性高。

Model control and the three test groups were given intravenous injections of Concanavalin A(20 mg/kg)0.5 mL respectively,once one day,for five days in all.

除正常对照组外，其余各组均尾静脉注射20 mg/kg的刀豆蛋白A0.5 mL，1次/d，连续5 d。

Breath, muscle contraction of the buttocks; arch body, as far as possible to hold his head, right leg straight towards the ceiling (peg-leg knee in order to avoid muscle tension).

呼气，收缩臀部肌肉；拱起身体，尽量抬起头来，右腿伸直朝向天花板（膝微屈，以避免肌肉紧张）。

The cost of moving grain food products was unchanged from May, but year over year are up 8%.

粮食产品的运输费用与5月份相比没有变化，但却比去年同期高8％。