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Results The CD4+ cell counts increased 22 cell/L (t=2.08, P=0.03) when compared with the baseline. IL-2 and IFN-γ increased 7.29pg/mL and 3.82pg/mL respectively (t=3.46, P=0.00; t=5.94, P=0.00), while IL-4 reduced 3.71pg/mL(t=8.18, P=0.00); HIVRNA deceased 0.40 lg copy/mL (t=2.65, P=0.02); 6 patients gained weight of more than 2 kg, 10 patients gained weight of no more than 2 kg. The results of blood mutine examination, liver and renal function were normal. No obvious adverse events were observed.

结果 治疗24周，CD4+细胞数较基线值(0周)平均上升22/μL(t=2.08，P=0.03)；细胞因子白细胞介素-2和干扰素-γ较基线值分别平均上升7.29pg/mL和3.82pg/mL（分别t=3.46，P=0.00；t=5.94，P=0.00），IL-4下降3.71pg/mL(t=8.18，P=0.00)；HIV RNA较基线值平均下降0.40lg拷贝／mL(t=2.65，P=0.02)；体重上升＞2kg者6例，增减未超过2kg者10例，患者体重评分有效率72.73%；血常规、肝肾功能等结果均在正常范围内；未观察到不良事件。

The irrigates were Chinese nutgall（200 mg/mL, 100 mg/mL, 50 mg/mL, 25 mg/mL, 12.5 mg/mL and 6.25 mg/mL）, 3 mL/L hydrogen peroxide plus physiological saline, 170 g/L EDTA plus 52.5 g/L NaCl0 and distilled water.

72个离体单根牙随机分为9组，冲洗液为五倍子组（分别为200、100、50、25、12.5、6.25mg／mL）、30mL／L过氧化氢液加生理盐水组、170g／LEDTA加52.5g／LNaOCl组、双蒸水组。

Medical ozone in different concentrations (10μg/ml, 30μg/ml, 50μg/ml, 80μg/ml, using or without using disposable gas filter needl) from ozonizer 20cm upper puff towards culture capsules for 20 minutes.

分别在培养皿上方20cm处发出浓度为10μg/ml、30μg/ml、50μg/ml、80μg/ml的医用臭氧，打开盖子20分钟后送培养。

Seedlings under light absorbed more rutin than those in the dark at rutin concentrations of 60-80 μg/mL and absorbed less rurin at concentrations lower than 40 μg/mL or near 100 μg/mL. IAA (＞0.1 μg/mL) treatment promoted rutin accumulation and inhibited elongation of shoot axis and primary roots.

当培养基中的芦丁浓度为60～80 μg/mL时，光照下的幼苗比暗处理的幼苗在胚轴中积累更多的芦丁；而芦丁浓度为40μg/mL以下和接近100μg/mL时幼苗在光照下累积的芦丁较暗处理的幼苗更少。0.1μg/mL以上的IAA促进芦丁的累积而进一步抑制幼苗胚轴和主根的伸长。

Methods: The DNA damage was measured after L-02 cell were incubated with 80μg/ml sodium fluoride, 1.73μg/ml selenium, 80μg/ml sodium fluoride and 1.73μg/ml selenium using Single Cell Electrophosis respectively.

采用80μg/ml氟化钠、1.73μg/ml亚硒酸钠以及80μg/ml氟化钠和1.73μg/ml亚硒酸钠对培养的L-02细胞进行染毒，24小时后用单细胞凝胶电泳技术检测各组L-02细胞DNA损伤情况。

Trophozoite,macrogamout,microgamout, typeⅠmeront,typeⅡmeront and sporozoite in the development of C.andersoni were observed by TEM.The proliferation of C.andersoni was significantly inhibited by 1.25μg/ml NTZ and 0.31μg/ml GAs.Treated with 20μg/ml NTZ and 5μg/ml GAs respectively,the numbers of C.andersoni were only 0.29%and 0.66%compared with the untreated.

透射电镜在HCT-8细胞中观察到安氏隐孢子虫的滋养体、雌配子体、雄配子体、Ⅰ型裂殖体、Ⅱ型裂殖体和子孢子阶段。1.25μg/ml NTZ和0.31μg/ml GAs即可显著抑制HCT-8细胞中安氏隐孢子虫的增殖。20μg/ml NTZ和5μg/ml GAs作用后，虫体数量仅分别为未加药的0.29%和0.66%。

The result of uroscopy of the proband was strong positive. There was a novel deletion mutation of c.876-877 del TC in the coding region of exon 6 of IDS gene, which was a hemizygous mutation. However, the mutation of his mother and sister was a heterozygous mutation. Detection of the exon 6 of IDS gene showed that the mutation was not found among normal controls and other patients with MPS I, IV, and VI other than MPS II. Homology comparison of amino acid sequences from different species showed that the phenylalanine glutamine of the mutation site of c.876-877 del TC located in p.292-293 was highly conserved. The activity of IDS enzyme of the proband was only 2.3 nmol/4 h/mL, which was much lower than normal; but the activity of IDS enzyme of his father, mother and sister was 641.9 nmol/4 h/mL, 95.8 nmol/4h/mL and 103.2 nmol/4h/mL, respectively.

结果显示：先证者尿检呈强阳性；其IDS基因exon 6编码区内存在c.876-877 del TC新缺失突变，为半合子突变，而其母、其姐为杂合突变；正常对照和其他非II型MPS患者的IDS基因exon 6的检测结果均未发现该突变；不同物种氨基酸序列的同源性比对显示： c.876-877 del TC突变所在的位置即p.292-293的苯丙氨酸谷氨酰胺高度保守；酶活性测定的结果显示：先证者的IDS酶活性仅为2.3 nmol/4 h/mL，大大低于正常值，而其父的为641.9 nmol/4 h/mL，其母的血浆酶活性为95.8 nmol/ 4h/mL，其姐的为103.2 nmol/4 h/mL。

The results showed that supplying lysine coated with the polyoxylateⅣand hydrogenated vegetable tallow in the rumen could significantly improve the concentration of plasma free lysine in the jugular vein (11.18ug/ml)(P＜0.05), which is about 66.61% of that with duodenal infusions of lysine(16.97ug/ml). The concentrations of free lysine were 7.31ug/ml and 8.00ug/ml with the basal diets and supplementing of Lys in the rumen respectively.

选用4头瘘管肉牛，采用4×4拉丁方设计，结果表明，瘤胃补充用丙烯酸树脂Ⅳ号和氢化植物油保护的赖氨酸26g(15g赖氨酸)，可显著提高肉牛血浆游离赖氨酸浓度(11.18ug／ml)，为十二指肠灌注15g赖氨酸(16.97ug／ml)的66.61％，基础日粮和瘤胃补充15g赖氨酸血浆游离赖氨酸浓度分别为7.31ug／ml，8.00ug／ml。

The results showed that:The best zymolysis conditions for BS and TV protoplasts release and regeneration through orthogonal experiment were as follows: 1mg·mL~(-1) lysozyme treated BS protoplasts for 1.5 hours at 37℃ and 4 mg·mL~(-1) lywallzyme treated TV protoplasts for 2 hours at 30℃. In these conditions, the number of protoplasts release was respectively 8.87×10~8 units/mL and 8.62×10~7units/mL and the regeneration rate was respectively 54.1% and 29.3%.

研究结果表明：通过正交试验确定BS菌和TV菌原生质体制备过程中的最佳酶解条件分别为：BS菌在37℃酶解温度下，1mg/mL溶菌酶酶解1.5h，原生质体制备个数达到8.87×10~8个/mL，再生率为54.1%；TV菌在30℃酶解温度下，4mg/mL破壁酶酶解2h，原生质体制备个数为8.62×10~7个/mL，再生率为29.3%。

Under lucifugal condition,the ED50 and ED90 of direct virucidal activity of it to HSV-1F strain are 12.90 μg/ml and 26.20 μg/ml separately,the ED50 and ED90 of infection block action of it to HSV-1F strain are 24.13 μg/ml and 32.06 μg/ml respectively.

在避光条件下，对HSV-1F株直接杀灭的ED50及ED90分别为12.90 μg/ml和26.20 μg/ml；感染阻断的ED50和ED90依次为24.13 μg/ml以及32.06 μg/ml。

On the other hand, the more important thing is because the urban housing is a kind of heterogeneity products.

另一方面，更重要的是由于城市住房是一种异质性产品。

Climate histogram is the fall that collects place measure calm value, cent serves as cross axle for a few equal interval, the area that the frequency that the value appears according to place is accumulated and becomes will be determined inside each interval, discharge the graph that rise with post, also be called histogram.

气候直方图是将所收集的降水量测定值，分为几个相等的区间作为横轴，并将各区间内所测定值依所出现的次数累积而成的面积，用柱子排起来的图形，也叫做柱状图。