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Under the optimum concentration of 1.5% glycerol in fermentation medium and feeding of 1% glycerol as precursor after 60 h of incubation, the titer of 2,668 μg/ml was obtained in shake flask culture, an increase of 7.4 times compared with the original strain.

以XC-5-48菌株为试验菌株，当培养基中甘油初始浓度为1.5%，并且在发酵60h时补加1.0%的甘油，摇瓶发酵的效价可达2668μg/ml，较出发菌株(318μg/ml)提高了7.4倍。

Cells at 2～×10〓/ml were reload into 96 culture plate for experiment.

在试验当天、第1、2、3和4天加入浓度分别为0.1ng/ml和1ng/ml的外源性TGF-β1；以未作基因转染和TGF-β1处理的A2780和AD4细胞作为对照。

OBJECTIVE:To establish the RP-HPLC method for determination of methyl hydroxybenzoate and ethyl hydroxybenzoate in oral liquid preparations METHODS:The Nova-Park C18 column(3 9mm×150mm,5μm)was used The mobile phase consisted of acetonitrile-water(25∶75)at a flow rate of 1ml/min,the detection wavelength was set at 254nm and the sensitivity was set at 0 01 AUFS RESULTS:The linear ranges of methyl hydroxybenzoate and ethyl hydroxybenzoate were 0 4 412～13 2 360μg/ml(r=0 9 999) and 0 6 672～20 0 160μg/m...

目的 ：建立内服液体制剂中羟苯甲酯和羟苯乙酯的反相高效液相色谱测定方法。方法：色谱柱：Nova -ParkC18 柱(3 9mm×150mm ，5μm)；流动相：乙腈-水(25∶75)；流速：1ml/min ；检测波长：254nm ；灵敏度：0 01AUFS。结果：羟苯甲酯浓度在0 4412～13 2360μg/ml范围内线性关系良好(r=0 9999)；羟苯乙酯浓度在0 6672～20 0160μg/ml范围内线性关系良好(r=0 9998)。结论：该方法简便、快速、准确，可用于医院制剂中防腐剂的检测。

The solvent was removed under vacuum shortly after the addition was compleated and replaced by 1-Methyl-2-pyrrolidinone (5 mL) followed by t-butylamine (5 mL, 47.5 mmol).

加完后真空除去溶剂，然后加入1-甲基-2-吡咯烷酮(5 mL)作为溶剂，再加入叔丁胺。

ES cells were maintained on gelatin-coated dishes in Iscove's modified Dulbecco medium containing 0.1 mmol/L of nonessential amino acids, 2 mmol/L of L-glutamine, 0.1 mmol/L of monothioglycerol, 15% fetal bovine serum, and 1000 U/mL leukemia inhibitory factor. Embryonic bodies were firstly formed by ES cells with hang drip method in IMDM without LIF. Six-day-old EBs were trypsinized and the cells were resuspended at a concentration of （3-4）×109 L-1 cells per 1 mL of a 1:1 mixture of culture medium and Matrigel?

实验方法：将鼠胚胎干细胞株接种于被覆明胶的培养瓶内，加入含0.1 mmol/L非必需氨基酸、2 mmol/L L-谷酰胺、0.1 mmol/L二羟基丙硫醇、15%胎牛血清、1 000 U/mL白血病抑制因子的IMDM培养液。3 d后采用&悬滴法&使细胞在不含白血病抑制因子的上述IMDM培养液中形成胚胎体，6 d后胰酶消化得到胚胎干细胞，浓度调整为（3~4）×109 L-1，与Matrigel？

The potency of antigens were higher than 6.0 IU/mL. The antigen is suitable immunising horse for producing rabies immunoglobulin.Keywords : Vero cells;Horse serum;Antigen

第5，8天收获病毒滴度可稳定在7.0logLD50/ mL以上，灭活抗原具良好的抗原性，用NIH法测定效价达6.0IU/ mL以上，可用作抗原生产抗狂犬病血清。

A good linear relationship between the carbon content and absorbance was in the concentration range from 5 to 120μg/mL cabon.

碳量在5~120μg/mL范围内与吸光度呈良好的线性关系，方法的检出限为2.9μg/mL。

Shaking culture with the liquid containing 1.5×10^6 connidia per millilitre for 3.5 d at the temperature of (25±1)℃ and 120r/min resulted in max mycelial of 5.1mg/mL and amylase yield of 272.42U/mg. An initial range of pH 4~6 in the liquid culture was the most favorable to amylase production with the yields of 1377.20~1528.99 U/mg. The activity of E.

在孢子浓度为1.5×10^6个／mL的PD培养液中恒温(25±1)℃振荡(120r/min)培养3.5d，菌丝生物量最大达5.1mg/mL，产淀粉酶量达272.42U/mg；培养液初始pH4~6最有利淀粉酶的产生，产酶量为1377.20~1528.99U/mg菌丝。

The objective of this paper was to investigate the effect of different treatments on fermentation quality of Xinjiang small reed and its nutrition constituent at different growth periods (heading period, blooming, fruiting). The five kinds of ensiling treatments (Fresh grass ensilage, fresh grass +10.0 g or 15.0 g molasses/kg, fresh grass +2.0 mL or 3.0 mL formic acid/kg) were carried out in the same growth period and its fermentation qualtiy and nutriton constituent were compared with each other.

以不同生育期（抽穗期、开花期、结实期）的新疆小芦苇为实验材料，同一生育期采用鲜贮、鲜草＋糖蜜10和15 g/kg、鲜草＋甲酸2和3 mL/kg等5种青贮处理，并对其相互间发酵品质和营养成分进行比较。

The amount of bleeding during operation was between 20 to 80ml(mean 50 ml).There was no urethra injury and stomal leak.

手术平均时间210 min（180～240 min），术中平均失血50 ml（20～80 ml），无输尿管损伤，无吻合口漏，住院时间7～18天，随访1年，未见肿瘤复发、种植、转移。

However, as the name（read－only memory）implies, CD disks cannot be written onorchanged in any way.

然而，正如其名字所指出的那样，CD盘不能写，也不能用任何方式改变其内容。

Galvanizes steel pallet is mainly export which suits standard packing of European Union, the North America. galvanizes steel pallet is suitable to heavy rack. Pallet surface can design plate type, corrugated and the gap form, satisfies the different requirements.

镀锌钢托盘多用于出口，替代木托盘，免薰蒸，符合欧盟、北美各国对出口货物包装材料的法令要求；喷涂钢托盘适用于重载上货架之用，托盘表面根据需要制作成平板状、波纹状及间隔形式，满足不同的使用要求。