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The optimal technical conditions were resulted when lignin 5 g, epichiorohydrin 14 ml, trimethylamine solution 30mL, reaction temperature 60℃ and time 3.5 h.

得到较佳的反应条件为：木质素5g，环氧氯丙烷14 mL，三甲胺溶液（体积分数33%）30 mL，反应温度60℃，反应时间3.5h。

After treated the spinal cord slice at E18 ~ P0 with ChABC at a final concentration of 0.1 U/ml, CGRP-LI fibers in the superficial layers of DH and their branches were seen to extend ventrally instead. When the concentration of ChABC increased to 0.5 U/ml, the above changes were more obvious.

2用0.1 U/ml终浓度的ChABC处理E18 ~ P0的小鼠脊髓片后，原本由DH浅层外侧部向腹内侧延伸的CGRP-LI轴突转为向腹外侧延伸，由DH浅层内侧部向腹外侧延伸的轴突同原本向背侧延伸的分支一起变为向腹侧生长。

The optimal conditions for extraction of saponins PD from Akebia trifoliate were as follows: 80 ml of 70% ethanol, 90 min action duration, then extractived by n-butyl alcohol for 3 times, hydrolyzed with 36% hydrochloric acid for 4 h at 70%, and extractived by chloroform.

结论］三叶木通中常春藤皂苷的最佳提取工艺为：70%甲醇溶液80 ml超声提取90 min，水饱和正丁醇萃取3次，36%盐酸4 ml，70℃水浴回流水解4 h，氯仿萃取2次，合并氯仿层，旋转蒸干后甲醇溶解定容。

METHODS : Twenty－five relapsed APL patients were enrolled (M 15, F 10, 3 4 a±s 12 a), 10 mL of 0.1% arsenous acid solution+5% glucos e 500 mL was injected iv, dr ip, daily for 28－56 d.

复发的急性早幼粒细胞白血病25例(男性15例，女性10例；年龄34a±s 12 a)以亚砷酸10mg/d+5%葡萄糖注射液500 mL静脉滴注，28～56 d为一个疗程。

Was studied by Folin Ciocalteu colorimetry. The results showed that polyphenol content of C. album could be well calculated according to their colorime tric absorption at 765nm by applying Folin Ciocalteu reagent 1.5mL and 10% Na2CO3 6 mL,2 h at 30℃ for colorimetric reaction, and the linear range of standard curve was 7-70mg/L.The method established was very convenient, stable and accurate.Key words: Canarium album Rauesch.;polyphenol;colorimetry

结果表明：橄榄多酚提取液在加入Folin Ciocalteu试剂1.5 mL，10% Na2CO3 6 mL，反应时间2 h，温度30℃的条件下进行显色，并于765 nm处测定其吸光值，多酚质量浓度在7～70mg/L范围内与吸光值有良好的线性关系，根据此线性回归方程进行橄榄多酚的定量测定，该方法简单方便，稳定性好，准确度高。

Bacteria which concentration more than or equal to 10~2/ml in CSF can be enriched effectively by means of centrifugalization when the relative centrifugal force is 5753xg and centrifugalization time is 15min and the rate of positive cultivation of pathogenic bacteria in clinical specimen can be improved by centrifugalization with high speed.

离心法不能使脑脊液中浓度低于10~2/ml的细菌浓集；在离心力5753×g离心时间为15分钟的离心条件下，采用离心法能使脑脊液中浓度大于等于10~2/ml的细菌得到最佳浓集，提高临床标本中细菌培养的阳性率。

METHODS: The Chelerythrine was used as the experimental group with concentrations ranging from 24.4μg/ml to 390.6μg/ml prepared with BHI broth medium with contained 2% glucose, and BHI culture medium was used as the control group.

采用二倍稀释法，用含2%蔗糖的脑心浸萃液肉汤琼脂液体培养基，将白屈菜红碱稀释为从390.6μg/ml到24。

The frequency distributions of EC50 values of the 65 isolates were normally distributed for both conidial germination and mycelial growth. Thus, the mean EC50 values 0.042 6 ± 0.030 4 and 0.025 0±0. 012 9 μg/mL of the 65 isolates could be used as the sensitivity baselines for conidial germination and mycelial growth of P. digitatum to azoxystrobin, respectively.

敏感性频次分析结果表明，该65个菌株孢子萌发和菌丝生长对嘧菌酯的敏感性频率分布均符合正态分布，其EC50平均值0.042 6±0.030 4μg/mL和0.025 0±0.012 9μg/mL可分别作为柑橘绿霉病菌孢子萌发和菌丝生长对嘧菌酯的敏感基线。

The EC_(50) inhibiting mycelia growth was 0.2603±0.0541μg/ml.But the effect of JS399-19 on conidial germination was low.

抑制菌丝生长的EC_(50)平均为0.2603±0.0541μg／ml，而抑制孢子萌芽的EC_(50)达30.9286μg／ml。

As with rhIL-1β, rhTNFα(100ng/ ml) can also provoked ICAM-1 surface expression by HMC. We also found VCAM-1 mRNA expression by HMC in normal culture condition and exposure of mesangial cells to rhTNFαis associated with increased VCAM-1 mRNA lavels rapidly (4 hours).These exploration indicate (1) glomerular and tubular ICAM-1 expression level correlate with inflammatory degree of glomerulus and TNF-αexpression level (2) VCAM-1 may have certain role in patients of lupus nephritis and crescentic nephritis (3) Expression of ICAM-1 and VCAM-1 may be regulated in vivo by cytokines (TNFα and IL-1β).

与轻度系膜增生性肾炎病人血清中的VCAM-1水平比较狼疮肾炎病人血清中VCAM-1的水平显著增加；（3）rhIL-1β（25ng/ml）不但能增加ICAM-1 mRNA的表达，还增加ICAM-1蛋白的表达；rhTNFα（100ng／ml）对ICAM-1蛋白表达和VCAM-1 mRNA基因表达均有明显的上调作用，结果提示：肾小球和肾小管ICAM-1表达水平与肾小球炎症病变程度有关；VCAM-1在狼疮肾炎和新月体肾炎的发生发展中可能有重要作用；体内ICAM-1和VCAM-1的表达可能受TNFα和IL-1β的调节。

Breath, muscle contraction of the buttocks; arch body, as far as possible to hold his head, right leg straight towards the ceiling (peg-leg knee in order to avoid muscle tension).

呼气，收缩臀部肌肉；拱起身体，尽量抬起头来，右腿伸直朝向天花板（膝微屈，以避免肌肉紧张）。

The cost of moving grain food products was unchanged from May, but year over year are up 8%.

粮食产品的运输费用与5月份相比没有变化，但却比去年同期高8％。