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Ethanol yield could reach 20.52 g/L (74.5 %of theoretical yield) under the following conditions: fermentation temperature at 28 ℃, initial pH value as 5.0, 150 mL liquid loadage in 250 mL rotating flask, and rotating speed at 160 r /min, and 0.5 % corn steep liquor and 0.024 % urea. Tran. by YUE Yang

在28 ℃，初始pH值5.0，160 r/min，250 mL三角瓶装液量150 mL，木糖浓度60 g/L，玉米浆0.5 %，含尿素0.024 %的条件下，休哈塔假丝酵母发酵木糖能产乙醇20.52 g/L，得率达到理论值的74.5 %。

Under lucifugal condition,the ED50 and ED90 of direct virucidal activity of it to HSV-1F strain are 12.90 μg/ml and 26.20 μg/ml s

在避光条件下，对HSV-1F株直接杀灭的ED50及ED90分别为12.90 μg/ml和26.20 μg/ml；感染阻断的ED50和ED90依次为24.13 μg/ml以及32.06 μg/ml。

In the acute toxicity experiments, the 96h-LC50 were 1.0×105 cells·mL-1 for the intact algal solution and 8.0×104 cells·mL-1 for the lysate. In a 7 day subacute experiment on the larvae, higher concentrations of algal solution inhibited S. macrocephalus larvae. The toxicity of the algal solution was higher than that of the lysate at the same concentration.

在仔鱼96h的急性毒性实验中，微小亚历山大藻对仔鱼的96h理论半致死浓度分别为1.0×105 cell·mL-1、8.0×104 cell·mL-1，而在7d的亚急性实验中，高浓度藻细胞液对仔鱼的生长发育有一定的抑制作用，且相同浓度下，藻细胞液产生的毒性较藻细胞破碎液大。

In the acute toxicity experiments, the algal solution and breakup solution of the concentration of 96h-LC50 were 1.0×105cells/mL and 8.0×104cells/mL, respectively. In the subacute 7 days experiment of the larvae, higher of the algal solution has the inhibition effect on sparus macrocephalus larvae. The toxicity of algal solution was higher than that of breakup solution at the same concentration.

在仔鱼96h的急性毒性实验中，微小亚历山大藻对仔鱼的96h理论半致死密度分别为1.0105 cell·mL-1、8.0104 cell·mL-1，而在7d的亚急性实验中，高浓度藻细胞液对仔鱼的生长发育有一定的抑制作用，且相同浓度下，藻细胞液产生的毒性较藻细胞破碎液大。

The inhibition rate were -10.4%,-12.69%, 20.74%, 63.14% and 65.13% when the drug concentration levels were 0.389, 0.648, 1.08, 1.8 and 3mg/mL, respectively. The half inhibitory concentration of Marsdenia Tenacissima extract A was 1.86 mg/mL using regression method.

乌骨藤提取物B对HepG2细胞的最大无毒浓度大约为0.648mg/mL；药物浓度在0.389、0.648、1.08、1.8、3mg/mL时的抑制率分别为-10.4%、-12.69%、20.74%、63.14%、65.13%；用回归法求出乌骨藤提取物B对HepG2细胞增殖的半数抑制浓度为1.86mg/mL。

Micronucleus test results exhibited that significant differences in micronuclear rates were determined between 0.4 mL bone cement and 0.4 mL microsphere-coated bone cement groups and negative control group, but no significant difference was detected among other groups. This revealed that this microsphere-coated bone cement leaching liquor did not show significant cell heredity toxic effects. Nevertheless, microsphere-coated bone cement in the high concentration and high dosage groups exhibited low toxic effects, mainly showing increased hemolysis rate and micronuclear rate. Thus, it was necessary to control the dosage and concentration of microsphere-coated bone cement.

微核实验结果显示除0.4 mL骨水泥组和0.4 mL微球骨水泥组的微核率与阴性对照组差异有显著性意义，其余各组差异无显著性意义，说明该微球骨水泥的浸提液无明显细胞遗传毒性作用，但是高浓度和高剂量组的微球骨水泥仍有较低的毒性作用，主要表现为溶血率和微核率的提高，因此在应用时要适当控制微球骨水泥的剂量和浓度。

Stock Solution— Dissolve 34 g of monobasic potassium phosphate in about 500 mL of water contained in a 1000-mL volumetric flask.

贮备液：将34g磷酸二氢钾在1000mL容量瓶中溶解于约500mL水中。

Detection the early stage apoptosis cells by flow cytornetry Incubating SCL-1 cells in 6-well culture medium with 1×10~6/well, after 12～48hculture with the concentration of imiquimod 0μg/mL and 150μg/mL, gathering cellsgrowing along the wall and making monocellular suspension, washing in PBS for 2times, and then add 5μL Annexin V/FITC and 10μL PI solutions, reaction for 15min avoiding light, detection the early stage apoptosis cells by flow cytometry.

流式细胞仪测定细胞早期凋亡 SCL-1细胞以1×10~6个／孔接种于6孔培养板中，使用含0μg／mL、150μg／mL咪喹莫特的培养液培养12～48h，收集贴壁生长的细胞制成单细胞悬液，PBS洗两次，分别加入5μL Annexin V／FITC和10μL浓度20μg／mL的PI溶液，混匀后室温避光孵育15min，流式细胞仪检测，结果用Cell Quest软件分析。

When the concentration of L-phenylalanine was 6 g/L,the optimum values of volume ratio of organic phase to aqueous phase,reaction temperature,pH value and liquid volume were 1∶1,30 ℃,4.0 and 20 mL/250 mL respectively. Under above optimum conditions,the content of 2-phenylethylalcohol reached 3.0 g/L 48 h later,which increased by about 50% than that in monophasic aqueous system.

当初始底物浓度为6 g/L、水和油酸的相体积比为1∶1、反应温度30 ℃和pH值4.0、装液量20 mL/250 mL 的条件下，转化48 h，产物浓度达到3.0 g/L，比单水相提高了50%。

METHODS: Fetal liver was obtained sterilely. Monoplast suspension was collected by collagenase digestion and mechanical separation, and then centrifuged using 1.070 g/mL Percoll separating medium and 1.077 g/mL Ficoll separating medium. Cells achieved from the interface of separatory liquids were cultured in DMEM/F12 medium, supplemented with 0.1 volume fraction of fetal bovine serum, 20 μg/L hepatocyte growth factor and 40 μg/L epidermal growth factor, for 9 days.

无菌状态下取出胎儿肝脏，以胶原酶消化法和机械分离法获得胎儿肝脏来源单细胞悬液，分别采用1.070 g/mL Percoll分离液和1.077 g/mL Ficoll分离液进行密度梯度离心，吸取界层细胞，添加含体积分数为0.1 FBS、20 μg/L肝细胞生长因子、40 μg/L表皮生长因子的DMEM/F12新鲜培养基诱导9 d。

As of Tuesday, Google's results were still censored in China.

截至周二，谷歌的搜索结果仍受中国审查。

In order to make the positive action increase and negative one decrease, the sub-forces of the social factors must be adjusted to form a centripetal force.

在这一过程中，人的主体性发挥是社会有机体健康发展的灵魂。